Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Anais da Academia Brasileira de Ciências (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602793 |
Resumo: | ABSTRACT The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A fragment of approximately 200bp of the SRY gene was amplified in seven females with normal external genitalia. The percentage of 32% of chimeric individuals detected in the present study is similar to that observed for pure specimens of Callithrix. These data suggests that hybridization probably does not interfere with the occurrence of twin gestation, nor of chimerism. Although cytogenetics is the main tool to identify the two cell lineages present in cases of chimerism, the amplification of the SRY gene by PCR has proved to be more efficient to identify the Y chromosome in cases of chimeric female marmoset. |
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Anais da Academia Brasileira de Ciências (Online) |
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Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates)karyotypeSRYchimaerashybridizationABSTRACT The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A fragment of approximately 200bp of the SRY gene was amplified in seven females with normal external genitalia. The percentage of 32% of chimeric individuals detected in the present study is similar to that observed for pure specimens of Callithrix. These data suggests that hybridization probably does not interfere with the occurrence of twin gestation, nor of chimerism. Although cytogenetics is the main tool to identify the two cell lineages present in cases of chimerism, the amplification of the SRY gene by PCR has proved to be more efficient to identify the Y chromosome in cases of chimeric female marmoset.Academia Brasileira de Ciências2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602793Anais da Academia Brasileira de Ciências v.89 n.4 2017reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201720170484info:eu-repo/semantics/openAccessSILVA,MONIQUE O.M.ARMADA,JORGE LUÍS A. DEVERONA,CARLOS EDUARDO S.HELIODORO,GABRIELANOGUEIRA,DENISE M.eng2017-12-13T00:00:00Zoai:scielo:S0001-37652017000602793Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2017-12-13T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false |
dc.title.none.fl_str_mv |
Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) |
title |
Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) |
spellingShingle |
Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) SILVA,MONIQUE O.M. karyotype SRY chimaeras hybridization |
title_short |
Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) |
title_full |
Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) |
title_fullStr |
Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) |
title_full_unstemmed |
Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) |
title_sort |
Cytogenetics and Molecular Genetic Analysis of Chimerism in Marmosets (Callithrix: Primates) |
author |
SILVA,MONIQUE O.M. |
author_facet |
SILVA,MONIQUE O.M. ARMADA,JORGE LUÍS A. DE VERONA,CARLOS EDUARDO S. HELIODORO,GABRIELA NOGUEIRA,DENISE M. |
author_role |
author |
author2 |
ARMADA,JORGE LUÍS A. DE VERONA,CARLOS EDUARDO S. HELIODORO,GABRIELA NOGUEIRA,DENISE M. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
SILVA,MONIQUE O.M. ARMADA,JORGE LUÍS A. DE VERONA,CARLOS EDUARDO S. HELIODORO,GABRIELA NOGUEIRA,DENISE M. |
dc.subject.por.fl_str_mv |
karyotype SRY chimaeras hybridization |
topic |
karyotype SRY chimaeras hybridization |
description |
ABSTRACT The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A fragment of approximately 200bp of the SRY gene was amplified in seven females with normal external genitalia. The percentage of 32% of chimeric individuals detected in the present study is similar to that observed for pure specimens of Callithrix. These data suggests that hybridization probably does not interfere with the occurrence of twin gestation, nor of chimerism. Although cytogenetics is the main tool to identify the two cell lineages present in cases of chimerism, the amplification of the SRY gene by PCR has proved to be more efficient to identify the Y chromosome in cases of chimeric female marmoset. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602793 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602793 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0001-3765201720170484 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
publisher.none.fl_str_mv |
Academia Brasileira de Ciências |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciências v.89 n.4 2017 reponame:Anais da Academia Brasileira de Ciências (Online) instname:Academia Brasileira de Ciências (ABC) instacron:ABC |
instname_str |
Academia Brasileira de Ciências (ABC) |
instacron_str |
ABC |
institution |
ABC |
reponame_str |
Anais da Academia Brasileira de Ciências (Online) |
collection |
Anais da Academia Brasileira de Ciências (Online) |
repository.name.fl_str_mv |
Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC) |
repository.mail.fl_str_mv |
||aabc@abc.org.br |
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1754302864083124224 |