Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract

Detalhes bibliográficos
Autor(a) principal: SPERA,KAMILLE D.
Data de Publicação: 2019
Outros Autores: FIGUEIREDO,PATRÍCIA A., SANTOS,PAMELA C.E, BARBOSA,FERNANDO C., ALVES,CAIO P., DOKKEDAL,ANNE LÍGIA, SALDANHA,LUIZ LEONARDO, SILVA,LUCIANA P., FIGUEIREDO,CARLOS ROGÉRIO, FERREIRA,PAULO CESAR, SILVA,REGILDO M.G. DA
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais da Academia Brasileira de Ciências (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617
Resumo: Abstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03 mg RE/g dry extract. HPLC-PAD chromatogram revealed presence of flavones as majority compound in evaluated extract. The results allowed us to also infer that the hydroethanolic extract from seeds shows cytotoxic activity against B16F10 melanoma cells line and it has dose-and-time-dependency.
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spelling Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extractB16F10 melanoma cellsmicronucleus testchromosomal aberrationDPPH radical scavengingHPLC-DADAbstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03 mg RE/g dry extract. HPLC-PAD chromatogram revealed presence of flavones as majority compound in evaluated extract. The results allowed us to also infer that the hydroethanolic extract from seeds shows cytotoxic activity against B16F10 melanoma cells line and it has dose-and-time-dependency.Academia Brasileira de Ciências2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617Anais da Academia Brasileira de Ciências v.91 n.4 2019reponame:Anais da Academia Brasileira de Ciências (Online)instname:Academia Brasileira de Ciências (ABC)instacron:ABC10.1590/0001-3765201920180446info:eu-repo/semantics/openAccessSPERA,KAMILLE D.FIGUEIREDO,PATRÍCIA A.SANTOS,PAMELA C.EBARBOSA,FERNANDO C.ALVES,CAIO P.DOKKEDAL,ANNE LÍGIASALDANHA,LUIZ LEONARDOSILVA,LUCIANA P.FIGUEIREDO,CARLOS ROGÉRIOFERREIRA,PAULO CESARSILVA,REGILDO M.G. DAeng2019-12-10T00:00:00Zoai:scielo:S0001-37652019000700617Revistahttp://www.scielo.br/aabchttps://old.scielo.br/oai/scielo-oai.php||aabc@abc.org.br1678-26900001-3765opendoar:2019-12-10T00:00Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)false
dc.title.none.fl_str_mv Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
title Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
spellingShingle Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
SPERA,KAMILLE D.
B16F10 melanoma cells
micronucleus test
chromosomal aberration
DPPH radical scavenging
HPLC-DAD
title_short Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
title_full Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
title_fullStr Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
title_full_unstemmed Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
title_sort Genotoxicity, anti-melanoma and antioxidant activities of Hymenaea courbaril L. seed extract
author SPERA,KAMILLE D.
author_facet SPERA,KAMILLE D.
FIGUEIREDO,PATRÍCIA A.
SANTOS,PAMELA C.E
BARBOSA,FERNANDO C.
ALVES,CAIO P.
DOKKEDAL,ANNE LÍGIA
SALDANHA,LUIZ LEONARDO
SILVA,LUCIANA P.
FIGUEIREDO,CARLOS ROGÉRIO
FERREIRA,PAULO CESAR
SILVA,REGILDO M.G. DA
author_role author
author2 FIGUEIREDO,PATRÍCIA A.
SANTOS,PAMELA C.E
BARBOSA,FERNANDO C.
ALVES,CAIO P.
DOKKEDAL,ANNE LÍGIA
SALDANHA,LUIZ LEONARDO
SILVA,LUCIANA P.
FIGUEIREDO,CARLOS ROGÉRIO
FERREIRA,PAULO CESAR
SILVA,REGILDO M.G. DA
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv SPERA,KAMILLE D.
FIGUEIREDO,PATRÍCIA A.
SANTOS,PAMELA C.E
BARBOSA,FERNANDO C.
ALVES,CAIO P.
DOKKEDAL,ANNE LÍGIA
SALDANHA,LUIZ LEONARDO
SILVA,LUCIANA P.
FIGUEIREDO,CARLOS ROGÉRIO
FERREIRA,PAULO CESAR
SILVA,REGILDO M.G. DA
dc.subject.por.fl_str_mv B16F10 melanoma cells
micronucleus test
chromosomal aberration
DPPH radical scavenging
HPLC-DAD
topic B16F10 melanoma cells
micronucleus test
chromosomal aberration
DPPH radical scavenging
HPLC-DAD
description Abstract: Hymenaea courbaril has been used to treat different diseases, although its properties are yet to be scientifically validated. The objective of this study was to determine the cytotoxicity, genotoxicity, antigenotoxicity and antioxidant potentials of hydroethanolic extract from H. courbaril seeds. Therefore, for the cytotoxicity test an anti-melanoma assay was performed in B16F10 strain cells. The genotoxicity and antigenotoxicity was evaluated in bone marrow cells (Permit number: 002/2010) of mice, the antioxidant activity was determined by the DPPH test and the total flavonoid content was also determined. The hydroethanolic extract showed antigenotoxic effect and antioxidant activity. It was verified that total flavonoid content was 442.25±18.03 mg RE/g dry extract. HPLC-PAD chromatogram revealed presence of flavones as majority compound in evaluated extract. The results allowed us to also infer that the hydroethanolic extract from seeds shows cytotoxic activity against B16F10 melanoma cells line and it has dose-and-time-dependency.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700617
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0001-3765201920180446
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências v.91 n.4 2019
reponame:Anais da Academia Brasileira de Ciências (Online)
instname:Academia Brasileira de Ciências (ABC)
instacron:ABC
instname_str Academia Brasileira de Ciências (ABC)
instacron_str ABC
institution ABC
reponame_str Anais da Academia Brasileira de Ciências (Online)
collection Anais da Academia Brasileira de Ciências (Online)
repository.name.fl_str_mv Anais da Academia Brasileira de Ciências (Online) - Academia Brasileira de Ciências (ABC)
repository.mail.fl_str_mv ||aabc@abc.org.br
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