Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation

Detalhes bibliográficos
Autor(a) principal: Puscas,I.
Data de Publicação: 2001
Outros Autores: Coltau,M., Gilau,L., Pasca,R., Domuta,G., Baican,M., Hecht,A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300007
Resumo: We studied the relationship between alpha- and beta-adrenergic agonists and the activity of carbonic anhydrase I and II in erythrocyte, clinical and vessel studies. Kinetic studies were performed. Adrenergic agonists increased erythrocyte carbonic anhydrase as follows: adrenaline by 75%, noradrenaline by 68%, isoprenaline by 55%, and orciprenaline by 62%. The kinetic data indicated a non-competitive mechanism of action. In clinical studies carbonic anhydrase I from erythrocytes increased by 87% after noradrenaline administration, by 71% after orciprenaline and by 82% after isoprenaline. The increase in carbonic anhydrase I paralleled the increase in blood pressure. Similar results were obtained in vessel studies on piglet vascular smooth muscle. We believe that adrenergic agonists may have a dual mechanism of action: the first one consists of a catecholamine action on its receptor with the formation of a stimulus-receptor complex. The second mechanism proposed completes the first one. By this second component of the mechanism, the same stimulus directly acts on the carbonic anhydrase I isozyme (that might be functionally coupled with adrenergic receptors), so that its activation ensures an adequate pH for stimulus-receptor coupling for signal transduction into the cell, resulting in vasoconstriction.
id ABDC-1_cb96fdc5d6004f5b92b45a0197cef0d9
oai_identifier_str oai:scielo:S0100-879X2001000300007
network_acronym_str ABDC-1
network_name_str Brazilian Journal of Medical and Biological Research
repository_id_str
spelling Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activationadrenergic agonistscarbonic anhydrasearterial blood pressureWe studied the relationship between alpha- and beta-adrenergic agonists and the activity of carbonic anhydrase I and II in erythrocyte, clinical and vessel studies. Kinetic studies were performed. Adrenergic agonists increased erythrocyte carbonic anhydrase as follows: adrenaline by 75%, noradrenaline by 68%, isoprenaline by 55%, and orciprenaline by 62%. The kinetic data indicated a non-competitive mechanism of action. In clinical studies carbonic anhydrase I from erythrocytes increased by 87% after noradrenaline administration, by 71% after orciprenaline and by 82% after isoprenaline. The increase in carbonic anhydrase I paralleled the increase in blood pressure. Similar results were obtained in vessel studies on piglet vascular smooth muscle. We believe that adrenergic agonists may have a dual mechanism of action: the first one consists of a catecholamine action on its receptor with the formation of a stimulus-receptor complex. The second mechanism proposed completes the first one. By this second component of the mechanism, the same stimulus directly acts on the carbonic anhydrase I isozyme (that might be functionally coupled with adrenergic receptors), so that its activation ensures an adequate pH for stimulus-receptor coupling for signal transduction into the cell, resulting in vasoconstriction.Associação Brasileira de Divulgação Científica2001-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300007Brazilian Journal of Medical and Biological Research v.34 n.3 2001reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2001000300007info:eu-repo/semantics/openAccessPuscas,I.Coltau,M.Gilau,L.Pasca,R.Domuta,G.Baican,M.Hecht,A.eng2001-03-16T00:00:00Zoai:scielo:S0100-879X2001000300007Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2001-03-16T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
title Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
spellingShingle Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
Puscas,I.
adrenergic agonists
carbonic anhydrase
arterial blood pressure
title_short Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
title_full Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
title_fullStr Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
title_full_unstemmed Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
title_sort Catecholamine-induced vasoconstriction is sensitive to carbonic anhydrase I activation
author Puscas,I.
author_facet Puscas,I.
Coltau,M.
Gilau,L.
Pasca,R.
Domuta,G.
Baican,M.
Hecht,A.
author_role author
author2 Coltau,M.
Gilau,L.
Pasca,R.
Domuta,G.
Baican,M.
Hecht,A.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Puscas,I.
Coltau,M.
Gilau,L.
Pasca,R.
Domuta,G.
Baican,M.
Hecht,A.
dc.subject.por.fl_str_mv adrenergic agonists
carbonic anhydrase
arterial blood pressure
topic adrenergic agonists
carbonic anhydrase
arterial blood pressure
description We studied the relationship between alpha- and beta-adrenergic agonists and the activity of carbonic anhydrase I and II in erythrocyte, clinical and vessel studies. Kinetic studies were performed. Adrenergic agonists increased erythrocyte carbonic anhydrase as follows: adrenaline by 75%, noradrenaline by 68%, isoprenaline by 55%, and orciprenaline by 62%. The kinetic data indicated a non-competitive mechanism of action. In clinical studies carbonic anhydrase I from erythrocytes increased by 87% after noradrenaline administration, by 71% after orciprenaline and by 82% after isoprenaline. The increase in carbonic anhydrase I paralleled the increase in blood pressure. Similar results were obtained in vessel studies on piglet vascular smooth muscle. We believe that adrenergic agonists may have a dual mechanism of action: the first one consists of a catecholamine action on its receptor with the formation of a stimulus-receptor complex. The second mechanism proposed completes the first one. By this second component of the mechanism, the same stimulus directly acts on the carbonic anhydrase I isozyme (that might be functionally coupled with adrenergic receptors), so that its activation ensures an adequate pH for stimulus-receptor coupling for signal transduction into the cell, resulting in vasoconstriction.
publishDate 2001
dc.date.none.fl_str_mv 2001-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2001000300007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.34 n.3 2001
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
_version_ 1754302930928795648