Preparation and characterization of liposomes entrapping allergenic proteins
Autor(a) principal: | |
---|---|
Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322004000200002 |
Resumo: | This work presents results of the preparation and characterization of small unilamellar liposomes for entrapping allergenic proteins extracted from the biomass of Dreschlera (Helminthosporium) monoceras cultivated by solid fermentation. Protein was entrapped by the dehydration-rehydration method, using lyophilization of preformed liposomes in order to prevent their degradation The reconstitution of lyophilized liposomes by hydration, their capacity for entrapping allergenic proteins and their stability in plasma were analyzed. Liposomes were reconstituted in size by including trehalose sugar in the formulation. The protection of the liposomal membrane by trehalose was characterized by differential scanning calorimetry and X-ray diffraction. The reconstituted membrane had an osmotic behavior similar to that of nondehydrated ones. Allergenic proteins ranging in molecular weight from 14 to 170 kDa were entrapped in the lipid matrix with an efficiency of approximately 80%. These results are promising for producing liposomes by entrapping allergenic proteins from mold extracts, which can be useful for allergy therapy. |
id |
ABEQ-1_b011da591996a03453f1ba392fce4ff6 |
---|---|
oai_identifier_str |
oai:scielo:S0104-66322004000200002 |
network_acronym_str |
ABEQ-1 |
network_name_str |
Brazilian Journal of Chemical Engineering |
repository_id_str |
|
spelling |
Preparation and characterization of liposomes entrapping allergenic proteinsliposomestrehaloseallergenic proteinsallergyThis work presents results of the preparation and characterization of small unilamellar liposomes for entrapping allergenic proteins extracted from the biomass of Dreschlera (Helminthosporium) monoceras cultivated by solid fermentation. Protein was entrapped by the dehydration-rehydration method, using lyophilization of preformed liposomes in order to prevent their degradation The reconstitution of lyophilized liposomes by hydration, their capacity for entrapping allergenic proteins and their stability in plasma were analyzed. Liposomes were reconstituted in size by including trehalose sugar in the formulation. The protection of the liposomal membrane by trehalose was characterized by differential scanning calorimetry and X-ray diffraction. The reconstituted membrane had an osmotic behavior similar to that of nondehydrated ones. Allergenic proteins ranging in molecular weight from 14 to 170 kDa were entrapped in the lipid matrix with an efficiency of approximately 80%. These results are promising for producing liposomes by entrapping allergenic proteins from mold extracts, which can be useful for allergy therapy.Brazilian Society of Chemical Engineering2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322004000200002Brazilian Journal of Chemical Engineering v.21 n.2 2004reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322004000200002info:eu-repo/semantics/openAccessCabral,E. C. M.Zollner,R. L.Santana,M. H. A.eng2004-05-26T00:00:00Zoai:scielo:S0104-66322004000200002Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2004-05-26T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Preparation and characterization of liposomes entrapping allergenic proteins |
title |
Preparation and characterization of liposomes entrapping allergenic proteins |
spellingShingle |
Preparation and characterization of liposomes entrapping allergenic proteins Cabral,E. C. M. liposomes trehalose allergenic proteins allergy |
title_short |
Preparation and characterization of liposomes entrapping allergenic proteins |
title_full |
Preparation and characterization of liposomes entrapping allergenic proteins |
title_fullStr |
Preparation and characterization of liposomes entrapping allergenic proteins |
title_full_unstemmed |
Preparation and characterization of liposomes entrapping allergenic proteins |
title_sort |
Preparation and characterization of liposomes entrapping allergenic proteins |
author |
Cabral,E. C. M. |
author_facet |
Cabral,E. C. M. Zollner,R. L. Santana,M. H. A. |
author_role |
author |
author2 |
Zollner,R. L. Santana,M. H. A. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Cabral,E. C. M. Zollner,R. L. Santana,M. H. A. |
dc.subject.por.fl_str_mv |
liposomes trehalose allergenic proteins allergy |
topic |
liposomes trehalose allergenic proteins allergy |
description |
This work presents results of the preparation and characterization of small unilamellar liposomes for entrapping allergenic proteins extracted from the biomass of Dreschlera (Helminthosporium) monoceras cultivated by solid fermentation. Protein was entrapped by the dehydration-rehydration method, using lyophilization of preformed liposomes in order to prevent their degradation The reconstitution of lyophilized liposomes by hydration, their capacity for entrapping allergenic proteins and their stability in plasma were analyzed. Liposomes were reconstituted in size by including trehalose sugar in the formulation. The protection of the liposomal membrane by trehalose was characterized by differential scanning calorimetry and X-ray diffraction. The reconstituted membrane had an osmotic behavior similar to that of nondehydrated ones. Allergenic proteins ranging in molecular weight from 14 to 170 kDa were entrapped in the lipid matrix with an efficiency of approximately 80%. These results are promising for producing liposomes by entrapping allergenic proteins from mold extracts, which can be useful for allergy therapy. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322004000200002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322004000200002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322004000200002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.21 n.2 2004 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213171531350016 |