Detalhes bibliográficos
Autor(a) principal: |
Zhan,J. F. |
Data de Publicação: |
2013 |
Outros Autores: |
Jiang,S. T.,
Pan,L. J. |
Tipo de documento: |
Artigo
|
Idioma: |
eng |
Título da fonte: |
Brazilian Journal of Chemical Engineering |
Texto Completo: |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400004
|
Resumo: |
The paper presents the synthesis and performance of an immobilized phospholipase A1 with practical application for oil degumming. The polyvinyl alcohol (PVA) had a number of properties indicating this polymer as a good enzyme carrier. The combination with alginate made a macro-porous structure, evidenced by SEM analyses. When the process time in boric acid solution was 30 minutes, the results revealed that beads prepared with 10% (w/v) PVA and 2% (w/v) sodium alginate in 4% (w/v) boric acid and 2% (w/v) calcium chloride solution exhibited high immobilized enzyme activity, immobilization yield and stability. The pH and temperature optimum for the PVA-alginate immobilized phospholipase A1were 5.6 and 58 °C, respectively. The enzyme immobilized in the beads retained 50.37% of the initial activity in the eighth cycle. The enzyme biocatalyst immobilized in the beads retained 78.58% of the initial activity after storing 6 weeks at 4 °C. |