HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC)
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Materials research (São Carlos. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392005000100005 |
Resumo: | HEMA/MMA/EDMA (2-hydroxyethyl methacrylate/methyl methacrylate/ethyleneglycol methacrylate) copolymer beads have been synthesized for use in aqueous SEC. This porous chromatographic support consisted of a large average particle size (250 µm) and contained macropores with a median pore size of approximately 880 Å. The material was packed in glass and stainless columns to assess their chromatographic performance. The packed columns were calibrated using narrow molecular weight (MW) distribution standards (dextran) and exhibited a range of molecular weight separation between 40,000 and 2,000,000 daltons. The packing material showed the ability to separate large molecules through the size exclusion mechanism. |
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Materials research (São Carlos. Online) |
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HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC)hydrophilic acrylate packing materialssize exclusion chromatographymacromolecules separationHEMA/MMA/EDMA (2-hydroxyethyl methacrylate/methyl methacrylate/ethyleneglycol methacrylate) copolymer beads have been synthesized for use in aqueous SEC. This porous chromatographic support consisted of a large average particle size (250 µm) and contained macropores with a median pore size of approximately 880 Å. The material was packed in glass and stainless columns to assess their chromatographic performance. The packed columns were calibrated using narrow molecular weight (MW) distribution standards (dextran) and exhibited a range of molecular weight separation between 40,000 and 2,000,000 daltons. The packing material showed the ability to separate large molecules through the size exclusion mechanism.ABM, ABC, ABPol2005-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392005000100005Materials Research v.8 n.1 2005reponame:Materials research (São Carlos. Online)instname:Universidade Federal de São Carlos (UFSCAR)instacron:ABM ABC ABPOL10.1590/S1516-14392005000100005info:eu-repo/semantics/openAccessVianna-Soares,Cristina DuarteCherng-Ju,KimBorenstein,Michael Roberteng2005-03-24T00:00:00Zoai:scielo:S1516-14392005000100005Revistahttp://www.scielo.br/mrPUBhttps://old.scielo.br/oai/scielo-oai.phpdedz@power.ufscar.br1980-53731516-1439opendoar:2005-03-24T00:00Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR)false |
dc.title.none.fl_str_mv |
HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC) |
title |
HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC) |
spellingShingle |
HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC) Vianna-Soares,Cristina Duarte hydrophilic acrylate packing materials size exclusion chromatography macromolecules separation |
title_short |
HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC) |
title_full |
HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC) |
title_fullStr |
HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC) |
title_full_unstemmed |
HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC) |
title_sort |
HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC) |
author |
Vianna-Soares,Cristina Duarte |
author_facet |
Vianna-Soares,Cristina Duarte Cherng-Ju,Kim Borenstein,Michael Robert |
author_role |
author |
author2 |
Cherng-Ju,Kim Borenstein,Michael Robert |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Vianna-Soares,Cristina Duarte Cherng-Ju,Kim Borenstein,Michael Robert |
dc.subject.por.fl_str_mv |
hydrophilic acrylate packing materials size exclusion chromatography macromolecules separation |
topic |
hydrophilic acrylate packing materials size exclusion chromatography macromolecules separation |
description |
HEMA/MMA/EDMA (2-hydroxyethyl methacrylate/methyl methacrylate/ethyleneglycol methacrylate) copolymer beads have been synthesized for use in aqueous SEC. This porous chromatographic support consisted of a large average particle size (250 µm) and contained macropores with a median pore size of approximately 880 Å. The material was packed in glass and stainless columns to assess their chromatographic performance. The packed columns were calibrated using narrow molecular weight (MW) distribution standards (dextran) and exhibited a range of molecular weight separation between 40,000 and 2,000,000 daltons. The packing material showed the ability to separate large molecules through the size exclusion mechanism. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392005000100005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392005000100005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-14392005000100005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABM, ABC, ABPol |
publisher.none.fl_str_mv |
ABM, ABC, ABPol |
dc.source.none.fl_str_mv |
Materials Research v.8 n.1 2005 reponame:Materials research (São Carlos. Online) instname:Universidade Federal de São Carlos (UFSCAR) instacron:ABM ABC ABPOL |
instname_str |
Universidade Federal de São Carlos (UFSCAR) |
instacron_str |
ABM ABC ABPOL |
institution |
ABM ABC ABPOL |
reponame_str |
Materials research (São Carlos. Online) |
collection |
Materials research (São Carlos. Online) |
repository.name.fl_str_mv |
Materials research (São Carlos. Online) - Universidade Federal de São Carlos (UFSCAR) |
repository.mail.fl_str_mv |
dedz@power.ufscar.br |
_version_ |
1754212657789927424 |