Methodological adjustments to the tetrazolium test in rice seeds
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Seed Science |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372017000100041 |
Resumo: | Abstract: Reducing the execution time of the tetrazolium test is important because it is used for making decisions during the preharvest and this test takes approximately 24 h. Thus, the goal of this research was to study preconditioning and staining periods and concentration of tetrazolium salt, in order to reduce the evaluation time of rice seed viability by tetrazolium test. Three independent experiments were conducted. In the first and second experiments, six rice seed lots from the BR Irga 424 cultivar were used. In the first experiment, different concentrations of tetrazolium salt (0.1, 0.25, 0.5 and 1 %) and staining times (0.5, 1.0 and 2.0 h) were evaluated. In the second one, different hydration periods (0.5, 1.0, 2.0 and 4.0 h) at two temperatures (35 and 40 °C) were tested. In the third one, eight seed lots from the same cultivar were used, and the effectiveness of the modified tetrazolium test in the evaluation of rice seed viability was assesed. It is possible to carried out the tetrazolium test on rice using the hydration of peeled seeds for 1 h at 40 °C and staining for 1 h with a 0.25% salt concentration. |
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Methodological adjustments to the tetrazolium test in rice seedsphysiological qualityseed viabilityOryza sativa LAbstract: Reducing the execution time of the tetrazolium test is important because it is used for making decisions during the preharvest and this test takes approximately 24 h. Thus, the goal of this research was to study preconditioning and staining periods and concentration of tetrazolium salt, in order to reduce the evaluation time of rice seed viability by tetrazolium test. Three independent experiments were conducted. In the first and second experiments, six rice seed lots from the BR Irga 424 cultivar were used. In the first experiment, different concentrations of tetrazolium salt (0.1, 0.25, 0.5 and 1 %) and staining times (0.5, 1.0 and 2.0 h) were evaluated. In the second one, different hydration periods (0.5, 1.0, 2.0 and 4.0 h) at two temperatures (35 and 40 °C) were tested. In the third one, eight seed lots from the same cultivar were used, and the effectiveness of the modified tetrazolium test in the evaluation of rice seed viability was assesed. It is possible to carried out the tetrazolium test on rice using the hydration of peeled seeds for 1 h at 40 °C and staining for 1 h with a 0.25% salt concentration.ABRATES - Associação Brasileira de Tecnologia de Sementes2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372017000100041Journal of Seed Science v.39 n.1 2017reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/2317-1545v39n1169643info:eu-repo/semantics/openAccessCarvalho,Ireni LeitzkeMeneghello,Géri EduardoTunes,Lilian Madruga deJácome,Caroline CostaSoares,Vanessa Nogueiraeng2017-04-06T00:00:00Zoai:scielo:S2317-15372017000100041Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2017-04-06T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Methodological adjustments to the tetrazolium test in rice seeds |
title |
Methodological adjustments to the tetrazolium test in rice seeds |
spellingShingle |
Methodological adjustments to the tetrazolium test in rice seeds Carvalho,Ireni Leitzke physiological quality seed viability Oryza sativa L |
title_short |
Methodological adjustments to the tetrazolium test in rice seeds |
title_full |
Methodological adjustments to the tetrazolium test in rice seeds |
title_fullStr |
Methodological adjustments to the tetrazolium test in rice seeds |
title_full_unstemmed |
Methodological adjustments to the tetrazolium test in rice seeds |
title_sort |
Methodological adjustments to the tetrazolium test in rice seeds |
author |
Carvalho,Ireni Leitzke |
author_facet |
Carvalho,Ireni Leitzke Meneghello,Géri Eduardo Tunes,Lilian Madruga de Jácome,Caroline Costa Soares,Vanessa Nogueira |
author_role |
author |
author2 |
Meneghello,Géri Eduardo Tunes,Lilian Madruga de Jácome,Caroline Costa Soares,Vanessa Nogueira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Carvalho,Ireni Leitzke Meneghello,Géri Eduardo Tunes,Lilian Madruga de Jácome,Caroline Costa Soares,Vanessa Nogueira |
dc.subject.por.fl_str_mv |
physiological quality seed viability Oryza sativa L |
topic |
physiological quality seed viability Oryza sativa L |
description |
Abstract: Reducing the execution time of the tetrazolium test is important because it is used for making decisions during the preharvest and this test takes approximately 24 h. Thus, the goal of this research was to study preconditioning and staining periods and concentration of tetrazolium salt, in order to reduce the evaluation time of rice seed viability by tetrazolium test. Three independent experiments were conducted. In the first and second experiments, six rice seed lots from the BR Irga 424 cultivar were used. In the first experiment, different concentrations of tetrazolium salt (0.1, 0.25, 0.5 and 1 %) and staining times (0.5, 1.0 and 2.0 h) were evaluated. In the second one, different hydration periods (0.5, 1.0, 2.0 and 4.0 h) at two temperatures (35 and 40 °C) were tested. In the third one, eight seed lots from the same cultivar were used, and the effectiveness of the modified tetrazolium test in the evaluation of rice seed viability was assesed. It is possible to carried out the tetrazolium test on rice using the hydration of peeled seeds for 1 h at 40 °C and staining for 1 h with a 0.25% salt concentration. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372017000100041 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372017000100041 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2317-1545v39n1169643 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Journal of Seed Science v.39 n.1 2017 reponame:Journal of Seed Science instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABRATES |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABRATES |
institution |
ABRATES |
reponame_str |
Journal of Seed Science |
collection |
Journal of Seed Science |
repository.name.fl_str_mv |
Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
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1754212982445834240 |