Molecular characterization of breast cancer in young Brazilian women

Detalhes bibliográficos
Autor(a) principal: Carvalho,Leda Viegas de
Data de Publicação: 2010
Outros Autores: Pereira,Emílio Marcelo, Frappart,Lucien, Boniol,Mathieu, Bernardo,Wanderley Marques, Tarricone,Vicente, Tavtigian,Sean, Southey,Melissa Caroline
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Associação Médica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302010000300010
Resumo: OBJECTIVE: To evaluate the distribution of molecular subtypes of breast tumors diagnosed in young Brazilian women and to analyze the frequency of loss of heterozygocity (LOH) in BRCA1 among different molecular subtypes of early-onset breast cancer. METHODS: Samples from 72 cases of invasive breast carcinoma diagnosed in women aged between 19 and 40 years were evaluated using an immunohistochemical panel of biomarkers. Three intragenic BRCA1 locus microsatellites, D17S1322, D17S1323, and D17S855, were PCR amplified from matched normal (lymphocyte) and tumor DNAs for (LOH) analysis. RESULTS: We found 13 cases (18%) that had an immunohistochemical profile consistent with being basal-like. Forty cases (55%) were luminal A type; 11% (8 cases) were luminal B type, 13% (9 cases) were HER2-overexpressing tumors and two cases were ER-/HER2- carcinomas lacking basal marker expression. Four of the 16 informative cases at D17S1322, one of the four informative cases at D17S855, and none of the five informative cases at D17S1323 displayed LOH (four basal-like and one Luminal A). Microsatellite instability (MSI) at D17S855 and D17S1322 was found in two cases (one a basal-like and one Luminal A). CONCLUSION: In our study, basal-like tumor was the second most frequent molecular type among young Brazilian women and was only observed in women diagnosed under the age of 35 years. There was no significant difference of LOH at BRCA1 locus rates between basal-like breast tumors and not-basal-like breast tumors (p=0.62). LOH in BRCA1 and MSI in these breast cancers were not frequent but may indicate a small group of breast cancers with a specific molecular makeup.
id AMB-1_4b3d98f76b3d8feeb352c18a08ba8cb3
oai_identifier_str oai:scielo:S0104-42302010000300010
network_acronym_str AMB-1
network_name_str Revista da Associação Médica Brasileira (Online)
repository_id_str
spelling Molecular characterization of breast cancer in young Brazilian womenBreast neoplasmsImmunohistochemistryGenes, BRCA1Loss of heterozygosityMicrosatellite instabilityOBJECTIVE: To evaluate the distribution of molecular subtypes of breast tumors diagnosed in young Brazilian women and to analyze the frequency of loss of heterozygocity (LOH) in BRCA1 among different molecular subtypes of early-onset breast cancer. METHODS: Samples from 72 cases of invasive breast carcinoma diagnosed in women aged between 19 and 40 years were evaluated using an immunohistochemical panel of biomarkers. Three intragenic BRCA1 locus microsatellites, D17S1322, D17S1323, and D17S855, were PCR amplified from matched normal (lymphocyte) and tumor DNAs for (LOH) analysis. RESULTS: We found 13 cases (18%) that had an immunohistochemical profile consistent with being basal-like. Forty cases (55%) were luminal A type; 11% (8 cases) were luminal B type, 13% (9 cases) were HER2-overexpressing tumors and two cases were ER-/HER2- carcinomas lacking basal marker expression. Four of the 16 informative cases at D17S1322, one of the four informative cases at D17S855, and none of the five informative cases at D17S1323 displayed LOH (four basal-like and one Luminal A). Microsatellite instability (MSI) at D17S855 and D17S1322 was found in two cases (one a basal-like and one Luminal A). CONCLUSION: In our study, basal-like tumor was the second most frequent molecular type among young Brazilian women and was only observed in women diagnosed under the age of 35 years. There was no significant difference of LOH at BRCA1 locus rates between basal-like breast tumors and not-basal-like breast tumors (p=0.62). LOH in BRCA1 and MSI in these breast cancers were not frequent but may indicate a small group of breast cancers with a specific molecular makeup.Associação Médica Brasileira2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302010000300010Revista da Associação Médica Brasileira v.56 n.3 2010reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/S0104-42302010000300010info:eu-repo/semantics/openAccessCarvalho,Leda Viegas dePereira,Emílio MarceloFrappart,LucienBoniol,MathieuBernardo,Wanderley MarquesTarricone,VicenteTavtigian,SeanSouthey,Melissa Carolineeng2010-08-05T00:00:00Zoai:scielo:S0104-42302010000300010Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2010-08-05T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false
dc.title.none.fl_str_mv Molecular characterization of breast cancer in young Brazilian women
title Molecular characterization of breast cancer in young Brazilian women
spellingShingle Molecular characterization of breast cancer in young Brazilian women
Carvalho,Leda Viegas de
Breast neoplasms
Immunohistochemistry
Genes, BRCA1
Loss of heterozygosity
Microsatellite instability
title_short Molecular characterization of breast cancer in young Brazilian women
title_full Molecular characterization of breast cancer in young Brazilian women
title_fullStr Molecular characterization of breast cancer in young Brazilian women
title_full_unstemmed Molecular characterization of breast cancer in young Brazilian women
title_sort Molecular characterization of breast cancer in young Brazilian women
author Carvalho,Leda Viegas de
author_facet Carvalho,Leda Viegas de
Pereira,Emílio Marcelo
Frappart,Lucien
Boniol,Mathieu
Bernardo,Wanderley Marques
Tarricone,Vicente
Tavtigian,Sean
Southey,Melissa Caroline
author_role author
author2 Pereira,Emílio Marcelo
Frappart,Lucien
Boniol,Mathieu
Bernardo,Wanderley Marques
Tarricone,Vicente
Tavtigian,Sean
Southey,Melissa Caroline
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho,Leda Viegas de
Pereira,Emílio Marcelo
Frappart,Lucien
Boniol,Mathieu
Bernardo,Wanderley Marques
Tarricone,Vicente
Tavtigian,Sean
Southey,Melissa Caroline
dc.subject.por.fl_str_mv Breast neoplasms
Immunohistochemistry
Genes, BRCA1
Loss of heterozygosity
Microsatellite instability
topic Breast neoplasms
Immunohistochemistry
Genes, BRCA1
Loss of heterozygosity
Microsatellite instability
description OBJECTIVE: To evaluate the distribution of molecular subtypes of breast tumors diagnosed in young Brazilian women and to analyze the frequency of loss of heterozygocity (LOH) in BRCA1 among different molecular subtypes of early-onset breast cancer. METHODS: Samples from 72 cases of invasive breast carcinoma diagnosed in women aged between 19 and 40 years were evaluated using an immunohistochemical panel of biomarkers. Three intragenic BRCA1 locus microsatellites, D17S1322, D17S1323, and D17S855, were PCR amplified from matched normal (lymphocyte) and tumor DNAs for (LOH) analysis. RESULTS: We found 13 cases (18%) that had an immunohistochemical profile consistent with being basal-like. Forty cases (55%) were luminal A type; 11% (8 cases) were luminal B type, 13% (9 cases) were HER2-overexpressing tumors and two cases were ER-/HER2- carcinomas lacking basal marker expression. Four of the 16 informative cases at D17S1322, one of the four informative cases at D17S855, and none of the five informative cases at D17S1323 displayed LOH (four basal-like and one Luminal A). Microsatellite instability (MSI) at D17S855 and D17S1322 was found in two cases (one a basal-like and one Luminal A). CONCLUSION: In our study, basal-like tumor was the second most frequent molecular type among young Brazilian women and was only observed in women diagnosed under the age of 35 years. There was no significant difference of LOH at BRCA1 locus rates between basal-like breast tumors and not-basal-like breast tumors (p=0.62). LOH in BRCA1 and MSI in these breast cancers were not frequent but may indicate a small group of breast cancers with a specific molecular makeup.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302010000300010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302010000300010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-42302010000300010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Médica Brasileira
publisher.none.fl_str_mv Associação Médica Brasileira
dc.source.none.fl_str_mv Revista da Associação Médica Brasileira v.56 n.3 2010
reponame:Revista da Associação Médica Brasileira (Online)
instname:Associação Médica Brasileira (AMB)
instacron:AMB
instname_str Associação Médica Brasileira (AMB)
instacron_str AMB
institution AMB
reponame_str Revista da Associação Médica Brasileira (Online)
collection Revista da Associação Médica Brasileira (Online)
repository.name.fl_str_mv Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)
repository.mail.fl_str_mv ||ramb@amb.org.br
_version_ 1754212829244686336