CD144, CD146 and VEGFR-2 properly identify circulating endothelial cell

• Autor(a) principal: Flores-Nascimento,Mariane Cristina
• Data de Publicação: 2015
• Outros Autores: Alessio,Aline Morandi, Orsi,Fernanda Loureiro de Andrade, Annichino-Bizzacchi,Joyce Maria
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista brasileira de hematologia e hemoterapia (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000200098
• topic: E; n; d; o; t; h; e; l; i; a; l; c; e; l; l; s; C; D; 1; 4; 6; a; n; t; i; g; e; n; s

Studies evaluating circulating endothelial cells by flow cytometry are faced by a lack of con- sensus about the best combination of monoclonal antibodies to be used. The rarity of these cells in peripheral blood, which represent 0.01% of mononuclear cells, drastically increases this challenge. Objective: The aim of this study is to suggest some combinations of markers that would safely and properly identify these cells. Methods: Flow cytometry analysis of circulating endothelial cells was performed applying three different panels composed of different combinations of the CD144, CD146, CD31, CD133, CD45 and anti-Vascular endothelial growth factor receptor-2 antibodies. Results: In spite of the rarity of the events, they were detectable and presented similar interperson numbers of circulating endothelial cells. Conclusion: The combination of markers successfully identified the circulating endothelial cells in healthy individuals, with the use of three different panels confirming the obtained data as reliable.