Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000400005 |
Resumo: | Cryopreservation is a promising technique for long term conservation of sugarcane's genetic resources which could be used in breeding programs. Preculture is a key step for success this technique. The purpose of this study was to evaluate the effect of sucrose concentrations and preculture period on dessication tolerance of sugarcane tissue dehydrated to 30, 20 and 10 % moisture. Sugarcane shoot tips were encapsulated in sodium alginate and precultured in a liquid culture medium with 0.3, 0.5, and 0.75 M sucrose. Preculturing in 0.3 M sucrose was ideal to induce desiccation tolerance in tissue. This sucrose concentration reduced the percentage of electrolytic leakage. Shoot tips were sensitive to a sucrose concentration of 0.75 M, resulting in low survival rates after desiccation. Preculturing in 0.5 M sucrose resulted in a higher survival rate after drying for 5.7 and 7.45 h. The percentage of electrolytic leakage was high when shoot tips were desiccated after preculturing in 0.5 and 0.75 M sucrose. |
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Crop Breeding and Applied Biotechnology |
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Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation toleranceelectrolytic leakageshoot tipstissue cultureCryopreservation is a promising technique for long term conservation of sugarcane's genetic resources which could be used in breeding programs. Preculture is a key step for success this technique. The purpose of this study was to evaluate the effect of sucrose concentrations and preculture period on dessication tolerance of sugarcane tissue dehydrated to 30, 20 and 10 % moisture. Sugarcane shoot tips were encapsulated in sodium alginate and precultured in a liquid culture medium with 0.3, 0.5, and 0.75 M sucrose. Preculturing in 0.3 M sucrose was ideal to induce desiccation tolerance in tissue. This sucrose concentration reduced the percentage of electrolytic leakage. Shoot tips were sensitive to a sucrose concentration of 0.75 M, resulting in low survival rates after desiccation. Preculturing in 0.5 M sucrose resulted in a higher survival rate after drying for 5.7 and 7.45 h. The percentage of electrolytic leakage was high when shoot tips were desiccated after preculturing in 0.5 and 0.75 M sucrose.Crop Breeding and Applied Biotechnology2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000400005Crop Breeding and Applied Biotechnology v.11 n.4 2011reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/S1984-70332011000400005info:eu-repo/semantics/openAccessMelo,Cristiane GamaranoBarbosa,Márcio Henrique PereiraMotoike,Sérgio YoshimitsuSabino,Marcone VieiraVentrella,Marília ContinPeternelli,Luiz AlexandreOliveira,Márcio Antônio Rochaeng2012-01-06T00:00:00Zoai:scielo:S1984-70332011000400005Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2012-01-06T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance |
title |
Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance |
spellingShingle |
Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance Melo,Cristiane Gamarano electrolytic leakage shoot tips tissue culture |
title_short |
Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance |
title_full |
Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance |
title_fullStr |
Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance |
title_full_unstemmed |
Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance |
title_sort |
Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance |
author |
Melo,Cristiane Gamarano |
author_facet |
Melo,Cristiane Gamarano Barbosa,Márcio Henrique Pereira Motoike,Sérgio Yoshimitsu Sabino,Marcone Vieira Ventrella,Marília Contin Peternelli,Luiz Alexandre Oliveira,Márcio Antônio Rocha |
author_role |
author |
author2 |
Barbosa,Márcio Henrique Pereira Motoike,Sérgio Yoshimitsu Sabino,Marcone Vieira Ventrella,Marília Contin Peternelli,Luiz Alexandre Oliveira,Márcio Antônio Rocha |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Melo,Cristiane Gamarano Barbosa,Márcio Henrique Pereira Motoike,Sérgio Yoshimitsu Sabino,Marcone Vieira Ventrella,Marília Contin Peternelli,Luiz Alexandre Oliveira,Márcio Antônio Rocha |
dc.subject.por.fl_str_mv |
electrolytic leakage shoot tips tissue culture |
topic |
electrolytic leakage shoot tips tissue culture |
description |
Cryopreservation is a promising technique for long term conservation of sugarcane's genetic resources which could be used in breeding programs. Preculture is a key step for success this technique. The purpose of this study was to evaluate the effect of sucrose concentrations and preculture period on dessication tolerance of sugarcane tissue dehydrated to 30, 20 and 10 % moisture. Sugarcane shoot tips were encapsulated in sodium alginate and precultured in a liquid culture medium with 0.3, 0.5, and 0.75 M sucrose. Preculturing in 0.3 M sucrose was ideal to induce desiccation tolerance in tissue. This sucrose concentration reduced the percentage of electrolytic leakage. Shoot tips were sensitive to a sucrose concentration of 0.75 M, resulting in low survival rates after desiccation. Preculturing in 0.5 M sucrose resulted in a higher survival rate after drying for 5.7 and 7.45 h. The percentage of electrolytic leakage was high when shoot tips were desiccated after preculturing in 0.5 and 0.75 M sucrose. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332011000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-70332011000400005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.11 n.4 2011 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209185935917056 |