A novel A2 allele found in Leishmania (Leishmania) infantum chagasi
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100009 |
Resumo: | Visceral leishmaniasis (VL) is a widely spread zoonotic disease. In Brazil the disease is caused by Leishmania (Leishmania) infantum chagasi. Peridomestic sandflies acquire the etiological agent by feeding on blood of infected reservoir animals, such as dogs or wildlife. The disease is endemic in Brazil and epidemic foci have been reported in densely populated cities all over the country. Many clinical features of Leishmania infection are related to the host-parasite relationship, and many candidate virulence factors in parasites that cause VL have been studied such as A2 genes. The A2 gene was first isolated in 1994 and then in 2005 three new alleles were described in Leishmania (Leishmania) infantum. In the present study we amplified by polymerase chain reaction (PCR) and sequenced the A2 gene from the genome of a clonal population of L. (L.) infantum chagasi VL parasites. The L. (L.) infantum chagasi A2 gene was amplified, cloned, and sequenced in. The amplified fragment showed approximately 90% similarity with another A2 allele amplified in Leishmania (Leishmania) donovani and in L.(L.) infantum described in literature. However, nucleotide translation shows differences in protein amino acid sequence, which may be essential to determine the variability of A2 genes in the species of the L. (L.) donovani complex and represents an additional tool to help understanding the role this gene family may have in establishing virulence and immunity in visceral leishmaniasis. This knowledge is important for the development of more accurate diagnostic tests and effective tools for disease control. |
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A novel A2 allele found in Leishmania (Leishmania) infantum chagasiLeishmania (Leishmania) infantum chagasiA2 geneallelesequencingVisceral leishmaniasis (VL) is a widely spread zoonotic disease. In Brazil the disease is caused by Leishmania (Leishmania) infantum chagasi. Peridomestic sandflies acquire the etiological agent by feeding on blood of infected reservoir animals, such as dogs or wildlife. The disease is endemic in Brazil and epidemic foci have been reported in densely populated cities all over the country. Many clinical features of Leishmania infection are related to the host-parasite relationship, and many candidate virulence factors in parasites that cause VL have been studied such as A2 genes. The A2 gene was first isolated in 1994 and then in 2005 three new alleles were described in Leishmania (Leishmania) infantum. In the present study we amplified by polymerase chain reaction (PCR) and sequenced the A2 gene from the genome of a clonal population of L. (L.) infantum chagasi VL parasites. The L. (L.) infantum chagasi A2 gene was amplified, cloned, and sequenced in. The amplified fragment showed approximately 90% similarity with another A2 allele amplified in Leishmania (Leishmania) donovani and in L.(L.) infantum described in literature. However, nucleotide translation shows differences in protein amino acid sequence, which may be essential to determine the variability of A2 genes in the species of the L. (L.) donovani complex and represents an additional tool to help understanding the role this gene family may have in establishing virulence and immunity in visceral leishmaniasis. This knowledge is important for the development of more accurate diagnostic tests and effective tools for disease control.Colégio Brasileiro de Parasitologia Veterinária2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100009Revista Brasileira de Parasitologia Veterinária v.20 n.1 2011reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612011000100009info:eu-repo/semantics/openAccessOliveira,Trícia Maria Ferreira de SousaVasconcelos,Elton José Rosas deNakaghi,Andréa Cristina HigaDefina,Tânia Paula AquinoJusi,Márcia Mariza GomesBaldani,Cristiane DivanCruz,Ângela KayselMachado,Rosangela Zacariaseng2011-04-20T00:00:00Zoai:scielo:S1984-29612011000100009Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2011-04-20T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
A novel A2 allele found in Leishmania (Leishmania) infantum chagasi |
title |
A novel A2 allele found in Leishmania (Leishmania) infantum chagasi |
spellingShingle |
A novel A2 allele found in Leishmania (Leishmania) infantum chagasi Oliveira,Trícia Maria Ferreira de Sousa Leishmania (Leishmania) infantum chagasi A2 gene allele sequencing |
title_short |
A novel A2 allele found in Leishmania (Leishmania) infantum chagasi |
title_full |
A novel A2 allele found in Leishmania (Leishmania) infantum chagasi |
title_fullStr |
A novel A2 allele found in Leishmania (Leishmania) infantum chagasi |
title_full_unstemmed |
A novel A2 allele found in Leishmania (Leishmania) infantum chagasi |
title_sort |
A novel A2 allele found in Leishmania (Leishmania) infantum chagasi |
author |
Oliveira,Trícia Maria Ferreira de Sousa |
author_facet |
Oliveira,Trícia Maria Ferreira de Sousa Vasconcelos,Elton José Rosas de Nakaghi,Andréa Cristina Higa Defina,Tânia Paula Aquino Jusi,Márcia Mariza Gomes Baldani,Cristiane Divan Cruz,Ângela Kaysel Machado,Rosangela Zacarias |
author_role |
author |
author2 |
Vasconcelos,Elton José Rosas de Nakaghi,Andréa Cristina Higa Defina,Tânia Paula Aquino Jusi,Márcia Mariza Gomes Baldani,Cristiane Divan Cruz,Ângela Kaysel Machado,Rosangela Zacarias |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Trícia Maria Ferreira de Sousa Vasconcelos,Elton José Rosas de Nakaghi,Andréa Cristina Higa Defina,Tânia Paula Aquino Jusi,Márcia Mariza Gomes Baldani,Cristiane Divan Cruz,Ângela Kaysel Machado,Rosangela Zacarias |
dc.subject.por.fl_str_mv |
Leishmania (Leishmania) infantum chagasi A2 gene allele sequencing |
topic |
Leishmania (Leishmania) infantum chagasi A2 gene allele sequencing |
description |
Visceral leishmaniasis (VL) is a widely spread zoonotic disease. In Brazil the disease is caused by Leishmania (Leishmania) infantum chagasi. Peridomestic sandflies acquire the etiological agent by feeding on blood of infected reservoir animals, such as dogs or wildlife. The disease is endemic in Brazil and epidemic foci have been reported in densely populated cities all over the country. Many clinical features of Leishmania infection are related to the host-parasite relationship, and many candidate virulence factors in parasites that cause VL have been studied such as A2 genes. The A2 gene was first isolated in 1994 and then in 2005 three new alleles were described in Leishmania (Leishmania) infantum. In the present study we amplified by polymerase chain reaction (PCR) and sequenced the A2 gene from the genome of a clonal population of L. (L.) infantum chagasi VL parasites. The L. (L.) infantum chagasi A2 gene was amplified, cloned, and sequenced in. The amplified fragment showed approximately 90% similarity with another A2 allele amplified in Leishmania (Leishmania) donovani and in L.(L.) infantum described in literature. However, nucleotide translation shows differences in protein amino acid sequence, which may be essential to determine the variability of A2 genes in the species of the L. (L.) donovani complex and represents an additional tool to help understanding the role this gene family may have in establishing virulence and immunity in visceral leishmaniasis. This knowledge is important for the development of more accurate diagnostic tests and effective tools for disease control. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612011000100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612011000100009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.20 n.1 2011 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
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||zacariascbpv@fcav.unesp.br |
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1754208913963614208 |