Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100136 |
Resumo: | Horn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ≤ 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity. |
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Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, BrazilHorn flyinsecticide resistancemetabolic resistancekdrHorn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ≤ 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity.Colégio Brasileiro de Parasitologia Veterinária2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100136Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/S1984-29612013005000016info:eu-repo/semantics/openAccessBarros,Antonio Thadeu MedeirosSchumaker,Teresinha Tizu SatoKoller,Wilson WernerKlafke,Guilherme MarcondesAlbuquerque,Thais Aguiar deGonzalez,Rodrigoeng2015-11-24T00:00:00Zoai:scielo:S1984-29612013000100136Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2015-11-24T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil |
title |
Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil |
spellingShingle |
Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil Barros,Antonio Thadeu Medeiros Horn fly insecticide resistance metabolic resistance kdr |
title_short |
Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil |
title_full |
Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil |
title_fullStr |
Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil |
title_full_unstemmed |
Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil |
title_sort |
Mechanisms of pyrethroid resistance inHaematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil |
author |
Barros,Antonio Thadeu Medeiros |
author_facet |
Barros,Antonio Thadeu Medeiros Schumaker,Teresinha Tizu Sato Koller,Wilson Werner Klafke,Guilherme Marcondes Albuquerque,Thais Aguiar de Gonzalez,Rodrigo |
author_role |
author |
author2 |
Schumaker,Teresinha Tizu Sato Koller,Wilson Werner Klafke,Guilherme Marcondes Albuquerque,Thais Aguiar de Gonzalez,Rodrigo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Barros,Antonio Thadeu Medeiros Schumaker,Teresinha Tizu Sato Koller,Wilson Werner Klafke,Guilherme Marcondes Albuquerque,Thais Aguiar de Gonzalez,Rodrigo |
dc.subject.por.fl_str_mv |
Horn fly insecticide resistance metabolic resistance kdr |
topic |
Horn fly insecticide resistance metabolic resistance kdr |
description |
Horn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ≤ 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100136 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612013000100136 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1984-29612013005000016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
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1754208914498387968 |