Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28850 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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Almeida, Glaucia SouzaD'Avila, HeloisaAlmeida, Patricia E.Gomes, Tatiana LunaLiechocki, SallyWalzog, BarbaraHepper, IngridFaria Neto, Hugo Caire CastroBozza, Patrícia T.Melo, Christianne BandeiraMonteiro, Clarissa M. Maya2018-09-18T12:54:51Z2018-09-18T12:54:51Z2018ALMEIDA, Glaucia Souza et al. Leptin mediates In vivo neutrophil migration: involvement of tumor necrosis factor-Alpha and CXCL1. Frontiers in Immunology, v. 9, p. 1-10, Feb. 2018.1664-3224https://www.arca.fiocruz.br/handle/icict/2885010.3389/fimmu.2018.00111engFrontiers MediaLeptinaNeutrófilosInflamaçãoleptinneutrophilinflammationtumor necrosis factor-alphaCXCL1leukotriene B4Fator de Necrose Tumoral alfaLeucotrieno B4Quimiocina CXCL1Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Juiz de Fora, MG, Brasil / Universidade do Estado do Rio de Janeiro. Instituto de Aplicação Fernando Rodrigues da Silveira. Departamento de Ciências da Natureza. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Ludwig-Maximilians-Universität München. Biomedical Center. Walter Brendel Centre of Experimental Medicine. Department of Cardiovascular Physiology and Pathophysiology. Munich, Germany,Ludwig-Maximilians-Universität München. Biomedical Center. Walter Brendel Centre of Experimental Medicine. Department of Cardiovascular Physiology and Pathophysiology. Munich, Germany,Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Leptin directly activates macrophages and lymphocytes, but the role of leptin in neutrophil activation and migration is still controversial. Here, we investigate the in vivo mechanisms of neutrophil migration induced by leptin. The intraperitoneal injection of leptin (1 mg/kg) induces a time- and concentration-dependent neutrophil influx. We did not observe the enhancement of lipid bodies/droplets in neutrophils, after leptin treatment, as we had observed previously in peritoneal macrophages. The participation of leukotriene B4 (LTB4) in neutrophil recruitment triggered by leptin was investigated using different strategies. Leptin-induced neutrophil recruitment occurs both in the absence of 5-lipoxygenase activity in 5-lipoxygenase (5-LO)-/- mice and after the administration of either 5-LO inhibitor (Zileuton) or the LTB4 receptor antagonist (U-75302). Moreover, no direct induction of LTB4 by leptin could be observed. Neutrophil influx could not be prevented by the mammalian target of rapamycin (mTOR) inhibitor, rapamycin, contrasting with the leptin-induced signaling for lipid body formation in macrophage that is mTOR-dependent. Leptin administration led to tumor necrosis factor-alpha (TNFα) production by the peritoneal cells both in vivo and in vitro. In addition, neutrophil recruitment was inhibited in tumor necrosis factor receptor 1 (TNFR1-/-) mice, indicating a role for TNF in leptin-induced neutrophil recruitment to the peritoneal cavity. Leptin-induced neutrophil influx was PI3Kγ-dependent, as it was absent in PI3Kγ-/- mice. Accordingly, leptin induced the peritoneal cells to produce CXCL1, both in vivo and in vitro, and the neutrophil influx was ablated after using an antibody against CXCL1. Our results establish TNFα/TNFR1- and CXCL1-dependent signaling as important pathways for leptin-induced neutrophil migration in vivo.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28850/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALglaucia_almeida_etal_IOC_2018.pdfglaucia_almeida_etal_IOC_2018.pdfapplication/pdf1184484https://www.arca.fiocruz.br/bitstream/icict/28850/2/glaucia_almeida_etal_IOC_2018.pdfa54c837c09572e08d4b8efc6cee3d882MD52TEXTglaucia_almeida_etal_IOC_2018.pdf.txtglaucia_almeida_etal_IOC_2018.pdf.txtExtracted texttext/plain55538https://www.arca.fiocruz.br/bitstream/icict/28850/3/glaucia_almeida_etal_IOC_2018.pdf.txt07bd07c240a9f1cb8337fd3e34e00872MD53icict/288502023-09-05 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| dc.title.pt_BR.fl_str_mv |
Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1 |
| title |
Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1 |
| spellingShingle |
Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1 Almeida, Glaucia Souza Leptina Neutrófilos Inflamação leptin neutrophil inflammation tumor necrosis factor-alpha CXCL1 leukotriene B4 Fator de Necrose Tumoral alfa Leucotrieno B4 Quimiocina CXCL1 |
| title_short |
Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1 |
| title_full |
Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1 |
| title_fullStr |
Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1 |
| title_full_unstemmed |
Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1 |
| title_sort |
Leptin Mediates In Vivo Neutrophil Migration: Involvement of Tumor Necrosis Factor-Alpha and CXCL1 |
| author |
Almeida, Glaucia Souza |
| author_facet |
Almeida, Glaucia Souza D'Avila, Heloisa Almeida, Patricia E. Gomes, Tatiana Luna Liechocki, Sally Walzog, Barbara Hepper, Ingrid Faria Neto, Hugo Caire Castro Bozza, Patrícia T. Melo, Christianne Bandeira Monteiro, Clarissa M. Maya |
| author_role |
author |
| author2 |
D'Avila, Heloisa Almeida, Patricia E. Gomes, Tatiana Luna Liechocki, Sally Walzog, Barbara Hepper, Ingrid Faria Neto, Hugo Caire Castro Bozza, Patrícia T. Melo, Christianne Bandeira Monteiro, Clarissa M. Maya |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Almeida, Glaucia Souza D'Avila, Heloisa Almeida, Patricia E. Gomes, Tatiana Luna Liechocki, Sally Walzog, Barbara Hepper, Ingrid Faria Neto, Hugo Caire Castro Bozza, Patrícia T. Melo, Christianne Bandeira Monteiro, Clarissa M. Maya |
| dc.subject.other.pt_BR.fl_str_mv |
Leptina Neutrófilos Inflamação |
| topic |
Leptina Neutrófilos Inflamação leptin neutrophil inflammation tumor necrosis factor-alpha CXCL1 leukotriene B4 Fator de Necrose Tumoral alfa Leucotrieno B4 Quimiocina CXCL1 |
| dc.subject.en.pt_BR.fl_str_mv |
leptin neutrophil inflammation tumor necrosis factor-alpha CXCL1 leukotriene B4 |
| dc.subject.decs.pt_BR.fl_str_mv |
Fator de Necrose Tumoral alfa Leucotrieno B4 Quimiocina CXCL1 |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. |
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2018 |
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2018-09-18T12:54:51Z |
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2018-09-18T12:54:51Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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ALMEIDA, Glaucia Souza et al. Leptin mediates In vivo neutrophil migration: involvement of tumor necrosis factor-Alpha and CXCL1. Frontiers in Immunology, v. 9, p. 1-10, Feb. 2018. |
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https://www.arca.fiocruz.br/handle/icict/28850 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2018.00111 |
| identifier_str_mv |
ALMEIDA, Glaucia Souza et al. Leptin mediates In vivo neutrophil migration: involvement of tumor necrosis factor-Alpha and CXCL1. Frontiers in Immunology, v. 9, p. 1-10, Feb. 2018. 1664-3224 10.3389/fimmu.2018.00111 |
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