An improved anti-C3/IgG ELISA for quantification of soluble immune complexes

Detalhes bibliográficos
Autor(a) principal: Soares, Neci Matos
Data de Publicação: 2001
Outros Autores: Santiago, Mittermayer Barreto, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5536
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Soares, Neci MatosSantiago, Mittermayer BarretoPontes-de-Carvalho, Lain Carlos2012-09-19T21:34:04Z2012-09-19T21:34:04Z2001SOARES, N. M.; SANTIAGO, M. B.; PONTES-DE-CARVALHO, L. C. An improved anti-C3/IgG ELISA for quantification of soluble immune complexes. Journal of Immunological Methods, v. 249, n. 1-2, p. 199-205, Mar. 2001.0022-1759S0022-1759(00)00362-8https://www.arca.fiocruz.br/handle/icict/5536engAn improved anti-C3/IgG ELISA for quantification of soluble immune complexesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilEscola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilA semi-quantitative ELISA for complement-fixing, IgG-containing immune complexes (IC) is described. The assay is based on the insolubilization of IC by polyethyleneglycol, their capture by solid-phase anti-C3 antibodies, reaction with peroxidase-labeled anti-IgG antibodies and incubation with a chromogenic peroxidase substrate. It was markedly improved by the use of a single-step procedure which simultaneously washed and precipitated the insolubilized immune complexes. Intra-assay and inter-assay coefficients of variation were lower than 8.6 and 14.7%, respectively. As expected, higher levels of circulating immune complexes, in relation to healthy individuals, were found in patients with American visceral leishmaniasis (AVL), systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), with prevalences comparable to those described in the literature. The ELISA can be quickly assembled from reagents and plasticware widely available commercially, detects immune complexes fulfilling three different criteria and is more sensitive than a previously published method based on the same principles (detection limit for complement-sensitized aggregated IgG of 2 microg ml(-1) as compared with a detection limit above 16 microg ml(-1)).Immune complexesPolyethylene glycolELISAC3IgGComplexo Antígeno-Anticorpo/análiseComplemento C3/imunologiaEnsaio de Imunoadsorção Enzimática/métodosImunoglobulina G/imunologiaHumanosSensibilidade e Especificidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSoares NM An improved anti-C3....pdfSoares NM An improved anti-C3....pdfapplication/pdf191788https://www.arca.fiocruz.br/bitstream/icict/5536/1/Soares%20NM%20An%20improved%20anti-C3....pdfb78c44651d8f3a884fc8fc69cbb05f66MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5536/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTSoares NM An improved anti-C3....pdf.txtSoares NM An improved anti-C3....pdf.txtExtracted texttext/plain24653https://www.arca.fiocruz.br/bitstream/icict/5536/5/Soares%20NM%20An%20improved%20anti-C3....pdf.txt25a264082f49dce2f9b3b45db1dab2aeMD55THUMBNAILSoares NM An improved anti-C3....pdf.jpgSoares NM An improved anti-C3....pdf.jpgGenerated Thumbnailimage/jpeg2091https://www.arca.fiocruz.br/bitstream/icict/5536/4/Soares%20NM%20An%20improved%20anti-C3....pdf.jpg9448de010a7839abfe776f55b4989356MD54icict/55362023-03-15 14:32:54.994oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
spellingShingle An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
Soares, Neci Matos
Immune complexes
Polyethylene glycol
ELISA
C3
IgG
Complexo Antígeno-Anticorpo/análise
Complemento C3/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Imunoglobulina G/imunologia
Humanos
Sensibilidade e Especificidade
title_short An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title_full An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title_fullStr An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title_full_unstemmed An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title_sort An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
author Soares, Neci Matos
author_facet Soares, Neci Matos
Santiago, Mittermayer Barreto
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Santiago, Mittermayer Barreto
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
dc.contributor.author.fl_str_mv Soares, Neci Matos
Santiago, Mittermayer Barreto
Pontes-de-Carvalho, Lain Carlos
dc.subject.en.pt_BR.fl_str_mv Immune complexes
Polyethylene glycol
ELISA
C3
IgG
topic Immune complexes
Polyethylene glycol
ELISA
C3
IgG
Complexo Antígeno-Anticorpo/análise
Complemento C3/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Imunoglobulina G/imunologia
Humanos
Sensibilidade e Especificidade
dc.subject.decs.pt_BR.fl_str_mv Complexo Antígeno-Anticorpo/análise
Complemento C3/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Imunoglobulina G/imunologia
Humanos
Sensibilidade e Especificidade
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2012-09-19T21:34:04Z
dc.date.available.fl_str_mv 2012-09-19T21:34:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, N. M.; SANTIAGO, M. B.; PONTES-DE-CARVALHO, L. C. An improved anti-C3/IgG ELISA for quantification of soluble immune complexes. Journal of Immunological Methods, v. 249, n. 1-2, p. 199-205, Mar. 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5536
dc.identifier.issn.none.fl_str_mv 0022-1759
dc.identifier.other.none.fl_str_mv S0022-1759(00)00362-8
identifier_str_mv SOARES, N. M.; SANTIAGO, M. B.; PONTES-DE-CARVALHO, L. C. An improved anti-C3/IgG ELISA for quantification of soluble immune complexes. Journal of Immunological Methods, v. 249, n. 1-2, p. 199-205, Mar. 2001.
0022-1759
S0022-1759(00)00362-8
url https://www.arca.fiocruz.br/handle/icict/5536
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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