Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Lima, Loyze P. de
Data de Publicação: 2019
Outros Autores: Calderano, Simone G., Silva, Marcelo S. da, Araujo, Christiane Bezerra, Vasconcelos, Elton J. R., Iwai, Leo K., Pereira, Claudio A., Fragoso, Stenio Perdigão, Elias, Maria Carolina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34301
Resumo: Instituto Butantan. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brasil / Instituto Butantan. Centro de Pesquisa em Toxinas, Resposta Imune e Sinalização Celular. São Paulo, SP, Brasil.
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spelling Lima, Loyze P. deCalderano, Simone G.Silva, Marcelo S. daAraujo, Christiane BezerraVasconcelos, Elton J. R.Iwai, Leo K.Pereira, Claudio A.Fragoso, Stenio PerdigãoElias, Maria Carolina2019-07-19T18:54:09Z2019-07-19T18:54:09Z2019LIMA, Loyse P. de et al. Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi. Scientific Reports, v. 9, n. 2888, p. 1-16, 2019.2045-2322https://www.arca.fiocruz.br/handle/icict/3430110.1038/s41598-019-39348-22045-2322porNature ResearchTrypanosoma cruziDNA-Directed DNA PolymeraseDNA HelicasesDNA ReplicationADN Polimerasa Dirigida por ADNADN HelicasasReplicación del ADNDNA Polimerase Dirigida por DNADNA HelicasesReplicação do DNAOrtholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brasil / Instituto Butantan. Centro de Pesquisa em Toxinas, Resposta Imune e Sinalização Celular. São Paulo, SP, Brasil.Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil.Instituto Butantan. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brasil / Instituto Butantan. Centro de Pesquisa em Toxinas, Resposta Imune e Sinalização Celular. São Paulo, SP, Brasil.Instituto Buntantan. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brasil / Instituto Buntantan. Centro de Pesquisa em Toxinas, Resposta Imune e Sinalização Celular. São Paulo, SP, Brasil.Western University of Health Sciences. College of Veterinary Medicine. Pomona, CA, USA.Instituto Buntantan. Laboratório Especial de Toxinologia Aplicada. São Paulo, SP, Brasil.Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científcas y Técnicas. Instituto de Investigaciones Médicas A. Lanari. Laboratorio de Parasitología Molecular. Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Buntantan. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brasil / Instituto Buntantan. Centro de Pesquisa em Toxinas, Resposta Imune e Sinalização Celular. São Paulo, SP, Brasil.DNA polymerase theta (Polθ), a member of the DNA polymerase family A, exhibits a polymerase C-terminal domain, a central domain, and an N-terminal helicase domain. Polθ plays important roles in DNA repair via its polymerase domain, regulating genome integrity. In addition, in mammals, Polθ modulates origin firing timing and MCM helicase recruitment to chromatin. In contrast, as a model eukaryote, Trypanosoma cruzi exhibits two individual putative orthologs of Polθ in different genomic loci; one ortholog is homologous to the Polθ C-terminal polymerase domain, and the other is homologous to the Polθ helicase domain, called Polθ-polymerase and Polθ-helicase, respectively. A pull-down assay using the T. cruzi component of the prereplication complex Orc1/Cdc6 as bait captured Polθ-helicase from the nuclear extract. Orc1/Cdc6 and Polθ-helicase directly interacted, and Polθ-helicase presented DNA unwinding and ATPase activities. A T. cruzi strain overexpressing the Polθ-helicase domain exhibited a significantly decreased amount of DNA-bound MCM7 and impaired replication origin firing. Taken together, these data suggest that Polθ-helicase modulates DNA replication by directly interacting with Orc1/Cdc6, which reduces the binding of MCM7 to DNA and thereby impairs the firing of replication origins.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/34301/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs41598-019-393.pdfs41598-019-393.pdfapplication/pdf3531818https://www.arca.fiocruz.br/bitstream/icict/34301/2/s41598-019-393.pdf54fcef068a6037b8b99186517071d83bMD52TEXTs41598-019-393.pdf.txts41598-019-393.pdf.txtExtracted texttext/plain75270https://www.arca.fiocruz.br/bitstream/icict/34301/3/s41598-019-393.pdf.txt86514f5d362517cd523c1939e3ed3a57MD53icict/343012021-12-23 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dc.title.pt_BR.fl_str_mv Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
title Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
spellingShingle Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
Lima, Loyze P. de
Trypanosoma cruzi
DNA-Directed DNA Polymerase
DNA Helicases
DNA Replication
ADN Polimerasa Dirigida por ADN
ADN Helicasas
Replicación del ADN
DNA Polimerase Dirigida por DNA
DNA Helicases
Replicação do DNA
title_short Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
title_full Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
title_fullStr Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
title_full_unstemmed Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
title_sort Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi
author Lima, Loyze P. de
author_facet Lima, Loyze P. de
Calderano, Simone G.
Silva, Marcelo S. da
Araujo, Christiane Bezerra
Vasconcelos, Elton J. R.
Iwai, Leo K.
Pereira, Claudio A.
Fragoso, Stenio Perdigão
Elias, Maria Carolina
author_role author
author2 Calderano, Simone G.
Silva, Marcelo S. da
Araujo, Christiane Bezerra
Vasconcelos, Elton J. R.
Iwai, Leo K.
Pereira, Claudio A.
Fragoso, Stenio Perdigão
Elias, Maria Carolina
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Loyze P. de
Calderano, Simone G.
Silva, Marcelo S. da
Araujo, Christiane Bezerra
Vasconcelos, Elton J. R.
Iwai, Leo K.
Pereira, Claudio A.
Fragoso, Stenio Perdigão
Elias, Maria Carolina
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
DNA-Directed DNA Polymerase
DNA Helicases
DNA Replication
ADN Polimerasa Dirigida por ADN
ADN Helicasas
Replicación del ADN
DNA Polimerase Dirigida por DNA
DNA Helicases
Replicação do DNA
dc.subject.en.pt_BR.fl_str_mv DNA-Directed DNA Polymerase
DNA Helicases
DNA Replication
dc.subject.es.pt_BR.fl_str_mv ADN Polimerasa Dirigida por ADN
ADN Helicasas
Replicación del ADN
dc.subject.decs.pt_BR.fl_str_mv DNA Polimerase Dirigida por DNA
DNA Helicases
Replicação do DNA
description Instituto Butantan. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brasil / Instituto Butantan. Centro de Pesquisa em Toxinas, Resposta Imune e Sinalização Celular. São Paulo, SP, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-07-19T18:54:09Z
dc.date.available.fl_str_mv 2019-07-19T18:54:09Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Loyse P. de et al. Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi. Scientific Reports, v. 9, n. 2888, p. 1-16, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34301
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-019-39348-2
dc.identifier.eissn.none.fl_str_mv 2045-2322
identifier_str_mv LIMA, Loyse P. de et al. Ortholog of the polymerase theta helicase domain modulates DNA replication in Trypanosoma cruzi. Scientific Reports, v. 9, n. 2888, p. 1-16, 2019.
2045-2322
10.1038/s41598-019-39348-2
url https://www.arca.fiocruz.br/handle/icict/34301
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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