Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies

Detalhes bibliográficos
Autor(a) principal: Paredes, Bruno Diaz
Data de Publicação: 2019
Outros Autores: Martins, Gabriele Louise Soares, Azevedo, Carine Machado, Sampaio, Gabriela Louise de Almeida, Nonaka, Carolina Kymie Vasques, Silva, Katia Nunes da, Soares, Milena Botelho Pereira, Santos, Ricardo Ribeiro dos, Souza, Bruno Solano de Freitas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34544
Resumo: CNPq, CAPES, FAPESB, Ministry of Health, National Induced Pluripotent Stem Cell Biobank and the National Institute of Science and Technology for Regenerative Medicine.
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spelling Paredes, Bruno DiazMartins, Gabriele Louise SoaresAzevedo, Carine MachadoSampaio, Gabriela Louise de AlmeidaNonaka, Carolina Kymie VasquesSilva, Katia Nunes daSoares, Milena Botelho PereiraSantos, Ricardo Ribeiro dosSouza, Bruno Solano de Freitas2019-07-31T17:21:24Z2019-07-31T17:21:24Z2019PAREDES, Bruno Diaz et al. Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies. Stem Cell Research, v. 38, p. 1-5, 2019.1873-5061https://www.arca.fiocruz.br/handle/icict/3454410.1016/j.scr.2019.101454CNPq, CAPES, FAPESB, Ministry of Health, National Induced Pluripotent Stem Cell Biobank and the National Institute of Science and Technology for Regenerative Medicine.São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil.São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / National Institute of Science and Technology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil.São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. D'Or Institute for Research and Education. Salvador, BA, Brazil.Sickle cell disease (SCD) is one of the most prevalent and severe monogenetic disorders. Previously, we generated iPS cell lines from SCD patients. Here, we generated iPS cell lines from three age-, ethnicity- and gender-matched healthy individuals as control cell lines. Cell reprogramming was performed using erythroblasts expanded from PBMC by a non-integrative method. SCD-iPSC controls expressed pluripotency markers, presented a normal karyotype, were able to differentiate into the three germ layers in embryoid body spontaneous differentiation and confirmed to be integration-free. The cell lines generated here may be used as matched healthy controls for SCD studies.engElsevierDoença FalciformeHemoglobinopatiasCelulas IPSAnemia Sickle CellHemoglobinopathiesInduced Pluripotent Stem CellsGeneration of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34544/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALParedes, B.D. Generation of three... 2019.pdfParedes, B.D. 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dc.title.pt_BR.fl_str_mv Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
title Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
spellingShingle Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
Paredes, Bruno Diaz
Doença Falciforme
Hemoglobinopatias
Celulas IPS
Anemia Sickle Cell
Hemoglobinopathies
Induced Pluripotent Stem Cells
title_short Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
title_full Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
title_fullStr Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
title_full_unstemmed Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
title_sort Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies
author Paredes, Bruno Diaz
author_facet Paredes, Bruno Diaz
Martins, Gabriele Louise Soares
Azevedo, Carine Machado
Sampaio, Gabriela Louise de Almeida
Nonaka, Carolina Kymie Vasques
Silva, Katia Nunes da
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Souza, Bruno Solano de Freitas
author_role author
author2 Martins, Gabriele Louise Soares
Azevedo, Carine Machado
Sampaio, Gabriela Louise de Almeida
Nonaka, Carolina Kymie Vasques
Silva, Katia Nunes da
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Souza, Bruno Solano de Freitas
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paredes, Bruno Diaz
Martins, Gabriele Louise Soares
Azevedo, Carine Machado
Sampaio, Gabriela Louise de Almeida
Nonaka, Carolina Kymie Vasques
Silva, Katia Nunes da
Soares, Milena Botelho Pereira
Santos, Ricardo Ribeiro dos
Souza, Bruno Solano de Freitas
dc.subject.other.pt_BR.fl_str_mv Doença Falciforme
Hemoglobinopatias
Celulas IPS
topic Doença Falciforme
Hemoglobinopatias
Celulas IPS
Anemia Sickle Cell
Hemoglobinopathies
Induced Pluripotent Stem Cells
dc.subject.en.pt_BR.fl_str_mv Anemia Sickle Cell
Hemoglobinopathies
Induced Pluripotent Stem Cells
description CNPq, CAPES, FAPESB, Ministry of Health, National Induced Pluripotent Stem Cell Biobank and the National Institute of Science and Technology for Regenerative Medicine.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-07-31T17:21:24Z
dc.date.available.fl_str_mv 2019-07-31T17:21:24Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAREDES, Bruno Diaz et al. Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies. Stem Cell Research, v. 38, p. 1-5, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34544
dc.identifier.issn.pt_BR.fl_str_mv 1873-5061
dc.identifier.doi.none.fl_str_mv 10.1016/j.scr.2019.101454
identifier_str_mv PAREDES, Bruno Diaz et al. Generation of three control iPS cell lines for sickle cell disease studies by reprogramming erythroblasts from individuals without hemoglobinopathies. Stem Cell Research, v. 38, p. 1-5, 2019.
1873-5061
10.1016/j.scr.2019.101454
url https://www.arca.fiocruz.br/handle/icict/34544
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/34544/1/license.txt
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