Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17924 |
Resumo: | CAPES (Zika FastTrack), FINEP, FAPERJ and FAPESB. |
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Souza, Bruno Solano de FreitasSampaio, Gabriela Louise de AlmeidaPereira, Ciro Silveira eCampos, Gubio SoaresSardi, Silvia IFreitas, Luiz Antonio Rodrigues deFigueira, Claudio PereiraParedes, Bruno DNonaka, Carolina K VAzevedo, Carine MachadoRocha, Vinicius Pinto CostaBandeira, Antonio Carlos de AlbuquerqueOtero, Rosalia MendezSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2017-03-03T17:45:57Z2017-03-03T17:45:57Z2016SOUZA, B. S. F. et al. Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells. Scientific Reports, v. 6, p. 39775, 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/1792410.1038/srep39775CAPES (Zika FastTrack), FINEP, FAPERJ and FAPESB.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, BrasilSão Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Laboratory of Virology, Salvador, BA, BrasilFederal University of Bahia. Laboratory of Virology, Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilSão Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, BrasilHospital Couto Maia. Salvador, BA, BrasilFederal University of Rio de Janeiro. Institute of Biophysics Carlos Chagas Filho. Rio de Janeiro, RJ, BrasilSão Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / São Rafael Hospital. Center for Biotechnology and Cell Therapy. Salvador, BA, BrasilZika virus (ZIKV) infection has been associated with severe complications both in the developing and adult nervous system. To investigate the deleterious effects of ZIKV infection, we used human neural progenitor cells (NPC), derived from induced pluripotent stem cells (iPSC). We found that NPC are highly susceptible to ZIKV and the infection results in cell death. ZIKV infection led to a marked reduction in cell proliferation, ultrastructural alterations and induction of autophagy. Induction of apoptosis of Sox2(+) cells was demonstrated by activation of caspases 3/7, 8 and 9, and by ultrastructural and flow cytometry analyses. ZIKV-induced death of Sox2(+) cells was prevented by incubation with the pan-caspase inhibitor, Z-VAD-FMK. By confocal microscopy analysis we found an increased number of cells with supernumerary centrosomes. Live imaging showed a significant increase in mitosis abnormalities, including multipolar spindle, chromosome laggards, micronuclei and death of progeny after cell division. FISH analysis for chromosomes 12 and 17 showed increased frequency of aneuploidy, such as monosomy, trisomy and polyploidy. Our study reinforces the link between ZIKV and abnormalities in the developing human brain, including microcephaly.engNature Publishing GroupZika virusInfecçãoCérebroAnormalidades do Sistema NervosoHumanosMicrocefaliaZika VirusInfectionBrainAbnormalities nervous systemHumansMicrocephalyZika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17924/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSouza BSF Zika virus infection....pdfSouza BSF Zika virus infection....pdfapplication/pdf2171006https://www.arca.fiocruz.br/bitstream/icict/17924/2/Souza%20BSF%20Zika%20virus%20infection....pdf48c728c951ec8375bde42f1653ec7131MD52TEXTSouza BSF Zika virus infection....pdf.txtSouza BSF Zika virus infection....pdf.txtExtracted texttext/plain43956https://www.arca.fiocruz.br/bitstream/icict/17924/3/Souza%20BSF%20Zika%20virus%20infection....pdf.txt03ef84ba3b027adae89905b6c82bb088MD53icict/179242023-03-15 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dc.title.pt_BR.fl_str_mv |
Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells |
title |
Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells |
spellingShingle |
Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells Souza, Bruno Solano de Freitas Zika virus Infecção Cérebro Anormalidades do Sistema Nervoso Humanos Microcefalia Zika Virus Infection Brain Abnormalities nervous system Humans Microcephaly |
title_short |
Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells |
title_full |
Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells |
title_fullStr |
Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells |
title_full_unstemmed |
Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells |
title_sort |
Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells |
author |
Souza, Bruno Solano de Freitas |
author_facet |
Souza, Bruno Solano de Freitas Sampaio, Gabriela Louise de Almeida Pereira, Ciro Silveira e Campos, Gubio Soares Sardi, Silvia I Freitas, Luiz Antonio Rodrigues de Figueira, Claudio Pereira Paredes, Bruno D Nonaka, Carolina K V Azevedo, Carine Machado Rocha, Vinicius Pinto Costa Bandeira, Antonio Carlos de Albuquerque Otero, Rosalia Mendez Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
author_role |
author |
author2 |
Sampaio, Gabriela Louise de Almeida Pereira, Ciro Silveira e Campos, Gubio Soares Sardi, Silvia I Freitas, Luiz Antonio Rodrigues de Figueira, Claudio Pereira Paredes, Bruno D Nonaka, Carolina K V Azevedo, Carine Machado Rocha, Vinicius Pinto Costa Bandeira, Antonio Carlos de Albuquerque Otero, Rosalia Mendez Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Bruno Solano de Freitas Sampaio, Gabriela Louise de Almeida Pereira, Ciro Silveira e Campos, Gubio Soares Sardi, Silvia I Freitas, Luiz Antonio Rodrigues de Figueira, Claudio Pereira Paredes, Bruno D Nonaka, Carolina K V Azevedo, Carine Machado Rocha, Vinicius Pinto Costa Bandeira, Antonio Carlos de Albuquerque Otero, Rosalia Mendez Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
dc.subject.other.pt_BR.fl_str_mv |
Zika virus Infecção Cérebro Anormalidades do Sistema Nervoso Humanos Microcefalia |
topic |
Zika virus Infecção Cérebro Anormalidades do Sistema Nervoso Humanos Microcefalia Zika Virus Infection Brain Abnormalities nervous system Humans Microcephaly |
dc.subject.en.pt_BR.fl_str_mv |
Zika Virus Infection Brain Abnormalities nervous system Humans Microcephaly |
description |
CAPES (Zika FastTrack), FINEP, FAPERJ and FAPESB. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-03-03T17:45:57Z |
dc.date.available.fl_str_mv |
2017-03-03T17:45:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SOUZA, B. S. F. et al. Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells. Scientific Reports, v. 6, p. 39775, 2016. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/17924 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.doi.none.fl_str_mv |
10.1038/srep39775 |
identifier_str_mv |
SOUZA, B. S. F. et al. Zika virus infection induces mitosis abnormalities and apoptotic cell death of human neural progenitor cells. Scientific Reports, v. 6, p. 39775, 2016. 2045-2322 10.1038/srep39775 |
url |
https://www.arca.fiocruz.br/handle/icict/17924 |
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eng |
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info:eu-repo/semantics/openAccess |
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Nature Publishing Group |
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Nature Publishing Group |
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