Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/13067 |
Resumo: | Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil. |
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Moreira, Marcela de LimaMoreira, Marcela de LimaDorneles, Elaine Maria SelesSoares, Rodrigo Pedro PintoMagalhães, Camila PaulaPereira, Christiane CostaLage, Andrey PereiraCarvalho, Andréa Teixeira deMartins Filho, Olindo AssisAraújo, Márcio Sobreira Silva2016-03-10T11:56:54Z2016-03-10T11:56:54Z2015MOREIRA, Marcela de Lima et al. Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining. Acta Veterinaria Scandinavica, v. 57, n. 1, p. 1-12, 2015.0044-605Xhttps://www.arca.fiocruz.br/handle/icict/1306710.1186/s13028-015-0142-yengBioMed CentralCross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staininginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil.BACKGROUND: The process for obtaining monoclonal antibodies against a specific antigen is very laborious, involves sophisticated technologies and it is not available in most research laboratories. Considering that most cytokines remain partially conserved among species during evolution, the search for antibody cross-reactivity is an important strategy for immunological studies in veterinary medicine. In this context, the amino acid sequence from human and canine cytokines have demonstrated 49-96 % homology, suggesting high probability of cross-reactivity amongst monoclonal antibodies. For this, 17 commercially available anti-human monoclonal antibodies [IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8 (#1, #2), IL-10, IL-12, IL-13, IL-17A, IFN-γ (#1, #2), TNF-α (#1, #2) and TGF-β], were evaluated in vitro for intracellular cytokine detection in a stimulated canine blood culture by flow cytometry and confocal microscopy. Lymphocytes from peripheral blood of healthy and two unhealthy dogs were analyzed. RESULTS: Eleven anti-human mAbs [IL-1α, IL-4, IL-5, IL-6, IL-8 (#1, #2), IL-12, IL-17A, TNF-α (#1, #2) and TGF-β] cross-reacted against canine intracellular cytokines. The specificity of the assays was not affected after Fc-blocking. Three anti-human cytokine mAbs [IL-4, IL-8 (#2) and TGF-β] when evaluated by confocal microscopy also cross-reacted with intracellular canine cytokines. The identification of human mAbs that cross-reacted with canine cytokines may support their use as immunological biomarkers in veterinary medicine studies. CONCLUSION: The identification of these 11 anti-human cytokine mAbs that cross-reacted with canine cytokines will be useful immunological biomarkers for pathological conditions by flow cytometry and fluorescence microscopy in dogs.CytokinesCanineCross reactivityFlow cytometryinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13067/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCross-reactivity of commercially (...)cytokines.pdfCross-reactivity of commercially (...)cytokines.pdfapplication/pdf5594254https://www.arca.fiocruz.br/bitstream/icict/13067/2/Cross-reactivity%20of%20commercially%20%28...%29cytokines.pdf95fd73371deb18ac4aebe2713c6fe691MD52TEXTCross-reactivity of commercially (...)cytokines.pdf.txtCross-reactivity of commercially (...)cytokines.pdf.txtExtracted 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Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining |
| title |
Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining |
| spellingShingle |
Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining Moreira, Marcela de Lima Cytokines Canine Cross reactivity Flow cytometry |
| title_short |
Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining |
| title_full |
Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining |
| title_fullStr |
Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining |
| title_full_unstemmed |
Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining |
| title_sort |
Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining |
| author |
Moreira, Marcela de Lima |
| author_facet |
Moreira, Marcela de Lima Dorneles, Elaine Maria Seles Soares, Rodrigo Pedro Pinto Magalhães, Camila Paula Pereira, Christiane Costa Lage, Andrey Pereira Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Araújo, Márcio Sobreira Silva |
| author_role |
author |
| author2 |
Dorneles, Elaine Maria Seles Soares, Rodrigo Pedro Pinto Magalhães, Camila Paula Pereira, Christiane Costa Lage, Andrey Pereira Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Araújo, Márcio Sobreira Silva |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Moreira, Marcela de Lima Moreira, Marcela de Lima Dorneles, Elaine Maria Seles Soares, Rodrigo Pedro Pinto Magalhães, Camila Paula Pereira, Christiane Costa Lage, Andrey Pereira Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Araújo, Márcio Sobreira Silva |
| dc.subject.en.pt_BR.fl_str_mv |
Cytokines Canine Cross reactivity Flow cytometry |
| topic |
Cytokines Canine Cross reactivity Flow cytometry |
| description |
Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratório de Bacteriologia Aplicada. Belo Horizonte, MG, Brasil. |
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2015 |
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2015 |
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2016-03-10T11:56:54Z |
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2016-03-10T11:56:54Z |
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MOREIRA, Marcela de Lima et al. Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining. Acta Veterinaria Scandinavica, v. 57, n. 1, p. 1-12, 2015. |
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https://www.arca.fiocruz.br/handle/icict/13067 |
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0044-605X |
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10.1186/s13028-015-0142-y |
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MOREIRA, Marcela de Lima et al. Cross-reactivity of commercially available anti-human monoclonal antibodies with canine cytokines: Establishment of a reliable panel to detect the functional profile of peripheral blood lymphocytes by intracytoplasmic staining. Acta Veterinaria Scandinavica, v. 57, n. 1, p. 1-12, 2015. 0044-605X 10.1186/s13028-015-0142-y |
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BioMed Central |
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