Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30367 |
Resumo: | Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunofarmacologia. Belo Horizonte, MG, Brazil |
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Garcia, Cristiana C.Weston-Davies, WynneRusso, Remo de CastroTavares, Luciana P.Rachid, Milene A.Alves Filho, Jose C.Machado, Alexandre de Magalhães VieiraNunn, Miles A.Teixeira, Mauro Martins2018-12-03T16:51:21Z2018-12-03T16:51:21Z2013GARCIA, Cristiana C. et al. Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury. PLoS One, v. 8, n. 5, p. e64443, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/3036710.1371/journal.pone.0064443engPublic Library of Sciencevirus da gripeInfluenza virusComplement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injuryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunofarmacologia. Belo Horizonte, MG, BrazilVarleigh Ltd. London. United KingdomUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunofarmacologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Departamento de Fisiologia e Biofısica. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunofarmacologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunofarmacologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Patologia Geral. Belo Horizonte, MG, BrazilUniversidade de Sao Paulo. Faculdade de Medicina. Departamento de Farmacologia. Ribeirao Preto, SP, BrazilFundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brazil, Bernhard RyffelCentre for Ecology and Hydrology. Wallingford, United KingdomUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunofarmacologia. Belo Horizonte, MG, BrazilInfluenza virus A (IAV) causes annual epidemics and intermittent pandemics that affect millions of people worldwide. Potent inflammatory responses are commonly associated with severe cases of IAV infection. The complement system, an important mechanism of innate and humoral immune responses to infections, is activated during primary IAV infection and mediates, in association with natural IgM, viral neutralization by virion aggregation and coating of viral hemmagglutinin. Increased levels of the anaphylatoxin C5a were found in patients fatally infected with the most recent H1N1 pandemic virus. In this study, our aim was to evaluate whether targeting C5 activation alters inflammatory lung injury and viral load in a murine model of IAV infection. To address this question C57Bl/6j mice were infected intranasally with 104 PFU of the mouse adapted Influenza A virus A/WSN/33 (H1N1) or inoculated with PBS (Mock). We demonstrated that C5a is increased in bronchoalveolar lavage fluid (BALF) upon experimental IAV infection. To evaluate the role of C5, we used OmCI, a potent arthropod-derived inhibitor of C5 activation that binds to C5 and prevents release of C5a by complement. OmCI was given daily by intraperitoneal injection from the day of IAV infection until day 5. Treatment with OmCI only partially reduced C5a levels in BALF. However, there was significant inhibition of neutrophil and macrophage infiltration in the airways, Neutrophil Extracellular Traps (NETs) formation, death of leukocytes, lung epithelial injury and overall lung damage induced by the infection. There was no effect on viral load. Taken together, these data suggest that targeting C5 activation with OmCI during IAV infection could be a promising approach to reduce excessive inflammatory reactions associated with the severe forms of IAV infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30367/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALComplement C5 Activation during Influenza A Infection.pdfComplement C5 Activation during Influenza A Infection.pdfapplication/pdf1359813https://www.arca.fiocruz.br/bitstream/icict/30367/2/Complement%20C5%20Activation%20during%20Influenza%20A%20Infection.pdf558337567527bd863d018edefc590f4cMD52TEXTComplement C5 Activation during Influenza A Infection.pdf.txtComplement C5 Activation during Influenza A Infection.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury |
| title |
Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury |
| spellingShingle |
Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury Garcia, Cristiana C. virus da gripe Influenza virus |
| title_short |
Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury |
| title_full |
Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury |
| title_fullStr |
Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury |
| title_full_unstemmed |
Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury |
| title_sort |
Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury |
| author |
Garcia, Cristiana C. |
| author_facet |
Garcia, Cristiana C. Weston-Davies, Wynne Russo, Remo de Castro Tavares, Luciana P. Rachid, Milene A. Alves Filho, Jose C. Machado, Alexandre de Magalhães Vieira Nunn, Miles A. Teixeira, Mauro Martins |
| author_role |
author |
| author2 |
Weston-Davies, Wynne Russo, Remo de Castro Tavares, Luciana P. Rachid, Milene A. Alves Filho, Jose C. Machado, Alexandre de Magalhães Vieira Nunn, Miles A. Teixeira, Mauro Martins |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Garcia, Cristiana C. Weston-Davies, Wynne Russo, Remo de Castro Tavares, Luciana P. Rachid, Milene A. Alves Filho, Jose C. Machado, Alexandre de Magalhães Vieira Nunn, Miles A. Teixeira, Mauro Martins |
| dc.subject.other.pt_BR.fl_str_mv |
virus da gripe |
| topic |
virus da gripe Influenza virus |
| dc.subject.en.pt_BR.fl_str_mv |
Influenza virus |
| description |
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Bioquımica e Imunologia. Laboratorio de Imunofarmacologia. Belo Horizonte, MG, Brazil |
| publishDate |
2013 |
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2013 |
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2018-12-03T16:51:21Z |
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2018-12-03T16:51:21Z |
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GARCIA, Cristiana C. et al. Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury. PLoS One, v. 8, n. 5, p. e64443, 2013. |
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https://www.arca.fiocruz.br/handle/icict/30367 |
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1932-6203 |
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10.1371/journal.pone.0064443 |
| identifier_str_mv |
GARCIA, Cristiana C. et al. Complement C5 Activation during Influenza A Infection in Mice Contributes to Neutrophil Recruitment and Lung Injury. PLoS One, v. 8, n. 5, p. e64443, 2013. 1932-6203 10.1371/journal.pone.0064443 |
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