Amoebiasis distribution in the past: first steps using a immunoassay technique

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Marcelo Luiz Carvalho
Data de Publicação: 2004
Outros Autores: Silva, Valmir Laurentino, Andrade, Carlos Mauricio de, Reinhard, Karl Jan, Rocha, Gino Chaves da, Bailly, Matthieu Le, Bouchet, Françoise, Silva, Luiz Fernando Rocha Ferreira da, Araújo, Adauto José Goncalves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31540
Resumo: Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
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spelling Gonçalves, Marcelo Luiz CarvalhoSilva, Valmir LaurentinoAndrade, Carlos Mauricio deReinhard, Karl JanRocha, Gino Chaves daBailly, Matthieu LeBouchet, FrançoiseSilva, Luiz Fernando Rocha Ferreira daAraújo, Adauto José Goncalves de2019-02-11T13:58:15Z2019-02-11T13:58:15Z2004GONÇALVES, Marcelo Luiz Carvalho et al. Amoebiasis distribution in the past: first steps using an immunoassay technique. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 98, n. 2, p. 1-4, 2004.0035-9203https://www.arca.fiocruz.br/handle/icict/315401878-3503engTransactions of The Royal Society of Tropical Medicine and HygieneAmoebiasis distribution in the past: first steps using a immunoassay techniqueinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.University of Nebraska. School of Natural Resource Sciences. Lincoln, NE, USA.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil / Universite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, FranceUniversite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France.Universite de Reims. U. F. R. de Pharmacie. Laboratoire de Paleoparasitologie. Reims, France.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.The identification of parasites in ancient human faeces is compromised by differential preservation of identifiable parasite structures. However, protein molecules can survive the damage of the environment and can be detected even after centuries. In this paper it is shown that is possible to detect copro-antigen of Entamoeba histolytica in historic and prehistoric human faecal remains, using a commercially available enzyme immunoassay (ELISA) kit. The kit uses monoclonal antibody-peroxidase conjugate specific for E. histolytica adhesin. A total of 90 specimens of desiccated faeces found in mummies and ancient organic sediment from South America, North America, Africa, and Europe were examined. The ELISA detected 20 positive samples, dated to about 5300 years before present to the 19th Century ad. The positive samples are from archaeological sites in Argentina, USA, France, Belgium, and Switzerland. The detection of protozoan antigen using immunoassays is a reliable tool for the studies of intestinal parasites in the past.PaleoparasitologyCoprolitesAncient FaecesAmebiasisELISAAmebiasisEnzyme-Linked Immunosorbent Assayinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/31540/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALAmoebiasis.pdfapplication/pdf87301https://www.arca.fiocruz.br/bitstream/icict/31540/2/Amoebiasis.pdfb7ef476072b55356d681cba66adfccf2MD52TEXTAmoebiasis.pdf.txtAmoebiasis.pdf.txtExtracted texttext/plain16082https://www.arca.fiocruz.br/bitstream/icict/31540/3/Amoebiasis.pdf.txtddd8a0795b748121f9bbd1e20aa7bad6MD53icict/315402023-01-12 16:17:29.458oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-01-12T19:17:29Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Amoebiasis distribution in the past: first steps using a immunoassay technique
title Amoebiasis distribution in the past: first steps using a immunoassay technique
spellingShingle Amoebiasis distribution in the past: first steps using a immunoassay technique
Gonçalves, Marcelo Luiz Carvalho
Paleoparasitology
Coprolites
Ancient Faeces
Amebiasis
ELISA
Amebiasis
Enzyme-Linked Immunosorbent Assay
title_short Amoebiasis distribution in the past: first steps using a immunoassay technique
title_full Amoebiasis distribution in the past: first steps using a immunoassay technique
title_fullStr Amoebiasis distribution in the past: first steps using a immunoassay technique
title_full_unstemmed Amoebiasis distribution in the past: first steps using a immunoassay technique
title_sort Amoebiasis distribution in the past: first steps using a immunoassay technique
author Gonçalves, Marcelo Luiz Carvalho
author_facet Gonçalves, Marcelo Luiz Carvalho
Silva, Valmir Laurentino
Andrade, Carlos Mauricio de
Reinhard, Karl Jan
Rocha, Gino Chaves da
Bailly, Matthieu Le
Bouchet, Françoise
Silva, Luiz Fernando Rocha Ferreira da
Araújo, Adauto José Goncalves de
author_role author
author2 Silva, Valmir Laurentino
Andrade, Carlos Mauricio de
Reinhard, Karl Jan
Rocha, Gino Chaves da
Bailly, Matthieu Le
Bouchet, Françoise
Silva, Luiz Fernando Rocha Ferreira da
Araújo, Adauto José Goncalves de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gonçalves, Marcelo Luiz Carvalho
Silva, Valmir Laurentino
Andrade, Carlos Mauricio de
Reinhard, Karl Jan
Rocha, Gino Chaves da
Bailly, Matthieu Le
Bouchet, Françoise
Silva, Luiz Fernando Rocha Ferreira da
Araújo, Adauto José Goncalves de
dc.subject.en.pt_BR.fl_str_mv Paleoparasitology
Coprolites
Ancient Faeces
Amebiasis
ELISA
topic Paleoparasitology
Coprolites
Ancient Faeces
Amebiasis
ELISA
Amebiasis
Enzyme-Linked Immunosorbent Assay
dc.subject.decs.en.fl_str_mv Amebiasis
Enzyme-Linked Immunosorbent Assay
description Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Rio de Janeiro, RJ, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2019-02-11T13:58:15Z
dc.date.available.fl_str_mv 2019-02-11T13:58:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GONÇALVES, Marcelo Luiz Carvalho et al. Amoebiasis distribution in the past: first steps using an immunoassay technique. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 98, n. 2, p. 1-4, 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31540
dc.identifier.issn.pt_BR.fl_str_mv 0035-9203
dc.identifier.eissn.none.fl_str_mv 1878-3503
identifier_str_mv GONÇALVES, Marcelo Luiz Carvalho et al. Amoebiasis distribution in the past: first steps using an immunoassay technique. Transactions of The Royal Society of Tropical Medicine and Hygiene, v. 98, n. 2, p. 1-4, 2004.
0035-9203
1878-3503
url https://www.arca.fiocruz.br/handle/icict/31540
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dc.publisher.none.fl_str_mv Transactions of The Royal Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv Transactions of The Royal Society of Tropical Medicine and Hygiene
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