What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis

Detalhes bibliográficos
Autor(a) principal: Sampaio, Vanderson de Souza
Data de Publicação: 2017
Outros Autores: Rivas, Gustavo Bueno da Silva, Kobylinski, Kevin, Pinilla, Yudi Tatiana, Pimenta, Paulo Filemon Paolucci, Lima, José Bento Pereira, Bruno, Rafaela Vieira, Lacerda, Marcus Vinícius Guimarães, Monteiro, Wuelton Marcelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25068
Resumo: Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Escola de Ciências da Saúde. Manaus, AM, Brazil/Fundação de Vigilância em Saúde do Amazonas. Sala de Análise de Situação em Saúde. Manaus, AM, Brazil
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spelling Sampaio, Vanderson de SouzaRivas, Gustavo Bueno da SilvaKobylinski, KevinPinilla, Yudi TatianaPimenta, Paulo Filemon PaolucciLima, José Bento PereiraBruno, Rafaela VieiraLacerda, Marcus Vinícius GuimarãesMonteiro, Wuelton Marcelo2018-03-06T12:18:16Z2018-03-06T12:18:16Z2017SAMPAIO, Vanderson de Souza et al. What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis. Parasit Vectors, v. 10, n. 1, p. 623, 20171756-3305https://www.arca.fiocruz.br/handle/icict/2506810.1186/s13071-017-2563-0engBioMed Centralerradicação da malariacontrole de vetoresIvermectinAnopheles aquasalisMalaria eliminationVector controlIvermectinAnopheles aquasalisLocomotor activityWhat does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Escola de Ciências da Saúde. Manaus, AM, Brazil/Fundação de Vigilância em Saúde do Amazonas. Sala de Análise de Situação em Saúde. Manaus, AM, BrazilCitrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.Armed Forces Research Institute of Medical Sciences. Department of Entomology and Nematology. Bangkok, ThailandFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Escola de Ciências da Saúde. Manaus, AM, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Escola de Ciências da Saúde. Manaus, AM, Brazil/Fundação Oswaldo Cruz. Instituto de Pesquisa Leônidas & Maria Deane. Manaus, AM, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Escola de Ciências da Saúde. Manaus, AM, BrazilBackground: Malaria remains a major public health concern. Vector control measures based solely on insecticide treated nets (ITNs) and indoor residual spraying (IRS) have demonstrated not to be feasible for malaria elimination. It has been shown that ivermectin affects several aspects of Anopheles species biology. Along the Latin American seacoast, Anopheles aquasalis Curry plays an important role in malaria transmission. The observation of mosquitoes locomotor activity under laboratory conditions can reveal details of their daily activity rhythms, which is controlled by an endogenous circadian clock that seems to be influenced by external signals, such as light and temperature. In this study, we assessed basal locomotor activity and the effects of ivermectin on locomotor activity of the American malaria vector, An. aquasalis. Methods: Adult females of Anopheles aquasalis used in experiments were three to five days post-emergence. Blood from one single subject was used to provide mosquito meals by membrane feeding assays. Powdered ivermectin compound was used to achieve different concentrations of drug as previously described. Fully engorged mosquitoes were individually placed into glass tubes and provided with 10% sucrose. Each tube was placed into a Locomotor Activity Monitor (LAM). The LAMs were kept inside an incubator under a constant temperature and a 12:12 h light:dark cycle. The average locomotor activity was calculated as the mean number of movements performed per mosquito in the period considered. Intervals of time assessed were adapted from a previous study. One-way ANOVA tests were performed in order to compare means between groups. Additionally, Dunnett’s method was used for post-hoc pairwise means comparisons between each group and control. Stata software version 13 was used for the analysis. Results: Anopheles aquasalis showed a nocturnal and bimodal pattern for mosquitoes fed both control blood meals and sub-lethal concentrations of ivermectin. In this species, activity peaks occurred at the beginning of the photophase and scotophase in the control group. The nocturnal activity is evident and higher just after the evening peak and maintains basal levels of locomotion throughout the scotophase. In the entire group analysis, locomotor activity means of experimental sets were significantly lower than control for each period of time evaluated. In the survival group, the locomotor activity means of all treatment sets were lower than control mosquitoes for all intervals of time when both the whole period and scotophase were assessed. When the middle of scotophase was evaluated, means were significantly lower for LC15 and LC25, but not LC5. For the beginning of photophase period, significant differences were detected only between control and LC5. When both the photophase and scotophase were assessed alone, no significant differences were found. Mean locomotor activity was significantly lower for dead group when compared to survival group for all experimental sets when whole period, photophase, and scotophase were assessed. Conclusions: Ivermectin seems to decrease locomotor activity of An. aquasalis at sub-lethal concentrations. The effects on locomotor activity increase according at higher ivermectin concentrations and are most evident during the whole scotophase as well as in the beginning and in the end of this phase, and sub-lethal effects may still be observed in the photophase. Findings presented in this study demonstrate that sub-lethal ivermectin effects reduce mosquito locomotor activity, which could diminish vectorial capacity and therefore the malaria transmission.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/25068/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALSampaio VS What does not kill it makes it weaker.pdfSampaio VS What does not kill it makes it weaker.pdfapplication/pdf494373https://www.arca.fiocruz.br/bitstream/icict/25068/2/Sampaio%20VS%20What%20does%20not%20kill%20it%20makes%20it%20weaker.pdfec8bd9f145be19f9f344e5c4756f1470MD52TEXTSampaio VS What does not kill it makes it weaker.pdf.txtSampaio VS What does not kill it makes it weaker.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis
title What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis
spellingShingle What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis
Sampaio, Vanderson de Souza
erradicação da malaria
controle de vetores
Ivermectin
Anopheles aquasalis
Malaria elimination
Vector control
Ivermectin
Anopheles aquasalis
Locomotor activity
title_short What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis
title_full What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis
title_fullStr What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis
title_full_unstemmed What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis
title_sort What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis
author Sampaio, Vanderson de Souza
author_facet Sampaio, Vanderson de Souza
Rivas, Gustavo Bueno da Silva
Kobylinski, Kevin
Pinilla, Yudi Tatiana
Pimenta, Paulo Filemon Paolucci
Lima, José Bento Pereira
Bruno, Rafaela Vieira
Lacerda, Marcus Vinícius Guimarães
Monteiro, Wuelton Marcelo
author_role author
author2 Rivas, Gustavo Bueno da Silva
Kobylinski, Kevin
Pinilla, Yudi Tatiana
Pimenta, Paulo Filemon Paolucci
Lima, José Bento Pereira
Bruno, Rafaela Vieira
Lacerda, Marcus Vinícius Guimarães
Monteiro, Wuelton Marcelo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sampaio, Vanderson de Souza
Rivas, Gustavo Bueno da Silva
Kobylinski, Kevin
Pinilla, Yudi Tatiana
Pimenta, Paulo Filemon Paolucci
Lima, José Bento Pereira
Bruno, Rafaela Vieira
Lacerda, Marcus Vinícius Guimarães
Monteiro, Wuelton Marcelo
dc.subject.other.pt_BR.fl_str_mv erradicação da malaria
controle de vetores
Ivermectin
Anopheles aquasalis
topic erradicação da malaria
controle de vetores
Ivermectin
Anopheles aquasalis
Malaria elimination
Vector control
Ivermectin
Anopheles aquasalis
Locomotor activity
dc.subject.en.pt_BR.fl_str_mv Malaria elimination
Vector control
Ivermectin
Anopheles aquasalis
Locomotor activity
description Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Departamento de Ensino e Pesquisa. Manaus, AM, Brazil/Universidade do Estado do Amazonas. Escola de Ciências da Saúde. Manaus, AM, Brazil/Fundação de Vigilância em Saúde do Amazonas. Sala de Análise de Situação em Saúde. Manaus, AM, Brazil
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-03-06T12:18:16Z
dc.date.available.fl_str_mv 2018-03-06T12:18:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SAMPAIO, Vanderson de Souza et al. What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis. Parasit Vectors, v. 10, n. 1, p. 623, 2017
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25068
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-017-2563-0
identifier_str_mv SAMPAIO, Vanderson de Souza et al. What does not kill it makes it weaker: effects of sub-lethal concentrations of ivermectin on the locomotor activity of Anopheles aquasalis. Parasit Vectors, v. 10, n. 1, p. 623, 2017
1756-3305
10.1186/s13071-017-2563-0
url https://www.arca.fiocruz.br/handle/icict/25068
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dc.publisher.none.fl_str_mv BioMed Central
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