Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.

Detalhes bibliográficos
Autor(a) principal: Soares, Milena Botelho Pereira
Data de Publicação: 2003
Outros Autores: Bellintani, Moema Cortizo, Ribeiro, Ivone Maria, Tomassini, Therezinha Coelho Barbosa, Santos, Ricardo Ribeiro dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/4190
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
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spelling Soares, Milena Botelho PereiraBellintani, Moema CortizoRibeiro, Ivone MariaTomassini, Therezinha Coelho BarbosaSantos, Ricardo Ribeiro dos2012-07-18T18:18:01Z2012-07-18T18:18:01Z2003SOARES, M .B. et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 20030014-2999S0014-2999(02)02829-7https://www.arca.fiocruz.br/handle/icict/4190engInhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2003Centro de Pesquisas Gonçalo MonizFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo MonizSalvadorFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Physalis angulata L. is an annual herb widely used in popular medicine for the treatment of a variety of pathologies. Here, we tested immunomodulatory activities of physalins, seco-steroids purified from P. angulata extracts. Addition of physalins B, F or G, but not D, caused a reduction in nitric oxide production by macrophages stimulated with lipopolysaccaride and interferon-g. In the presence of physalin B, macrophages stimulated with lipopolysaccaride, alone or in combination with interferon-g, produced lower levels of tumour necrosis factor (TNF)-a, interleukin-6 and interleukin-12. The inhibitory activity of physalin B, unlike that of dexamethasone, was not reversed by RU486 [(4-dimethylamino) phenyl-17h-hydroxy-17-(1-propynyl)estra-4,9-dien-3-one], an antiglucocorticoid. Physalin B-treated mice had lower levels of serum TNF-a than control mice after lipopolysaccaride challenge. More importantly, mice injected with physalins B, F or G survived after a lethal lipopolysaccaride challenge. These results demonstrate that seco-steroids from P. angulata are potent immunomodulatory substances and act through a mechanism distinct from that of dexamethasone.SteroidImmunomodulation(Physalis angulata L.)Endotoxic shockLipopolissacarídeos/farmacologiaAtivação de Macrófagos/efeitos de drogasPhysalis/químicaFitosteróis/farmacologiaSobrevivência Celular/efeitos de drogasCélulas CultivadasCitocinas/sangueCitocinas/metabolismoRelação Dose-Resposta a DrogaAntagonistas de Hormônios/farmacologiaLactonas/farmacologiaMacrófagos Peritoneais/citologiaCamundongos Endogâmicos BALB CMifepristona/farmacologiaOxido Nítrico/sangueExtratos Vegetais/químicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSoares MB Inhibition of macrophage activation....pdfSoares MB Inhibition of macrophage activation....pdfapplication/pdf194748https://www.arca.fiocruz.br/bitstream/icict/4190/1/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf42b363a4b5df1780f7b7e551ff9992e6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81990https://www.arca.fiocruz.br/bitstream/icict/4190/2/license.txtb6ead4607e393dee1190c21ba08397beMD52TEXTSoares MB Inhibition of macrophage activation....pdf.txtSoares MB Inhibition of macrophage activation....pdf.txtExtracted texttext/plain25878https://www.arca.fiocruz.br/bitstream/icict/4190/5/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf.txt759ae2b03d483b345d99a5670d74c7d6MD55THUMBNAILSoares MB Inhibition of macrophage activation....pdf.jpgSoares MB Inhibition of macrophage activation....pdf.jpgGenerated Thumbnailimage/jpeg2291https://www.arca.fiocruz.br/bitstream/icict/4190/4/Soares%20MB%20Inhibition%20of%20macrophage%20activation....pdf.jpg1c71651d4321b266c787fff168a54784MD54icict/41902023-03-15 14:32:57.203oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
dc.title.original.pt_BR.fl_str_mv Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
title Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
spellingShingle Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
Soares, Milena Botelho Pereira
Steroid
Immunomodulation
(Physalis angulata L.)
Endotoxic shock
Lipopolissacarídeos/farmacologia
Ativação de Macrófagos/efeitos de drogas
Physalis/química
Fitosteróis/farmacologia
Sobrevivência Celular/efeitos de drogas
Células Cultivadas
Citocinas/sangue
Citocinas/metabolismo
Relação Dose-Resposta a Droga
Antagonistas de Hormônios/farmacologia
Lactonas/farmacologia
Macrófagos Peritoneais/citologia
Camundongos Endogâmicos BALB C
Mifepristona/farmacologia
Oxido Nítrico/sangue
Extratos Vegetais/química
title_short Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
title_full Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
title_fullStr Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
title_full_unstemmed Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
title_sort Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L.
author Soares, Milena Botelho Pereira
author_facet Soares, Milena Botelho Pereira
Bellintani, Moema Cortizo
Ribeiro, Ivone Maria
Tomassini, Therezinha Coelho Barbosa
Santos, Ricardo Ribeiro dos
author_role author
author2 Bellintani, Moema Cortizo
Ribeiro, Ivone Maria
Tomassini, Therezinha Coelho Barbosa
Santos, Ricardo Ribeiro dos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Soares, Milena Botelho Pereira
Bellintani, Moema Cortizo
Ribeiro, Ivone Maria
Tomassini, Therezinha Coelho Barbosa
Santos, Ricardo Ribeiro dos
dc.subject.en.pt_BR.fl_str_mv Steroid
Immunomodulation
(Physalis angulata L.)
Endotoxic shock
topic Steroid
Immunomodulation
(Physalis angulata L.)
Endotoxic shock
Lipopolissacarídeos/farmacologia
Ativação de Macrófagos/efeitos de drogas
Physalis/química
Fitosteróis/farmacologia
Sobrevivência Celular/efeitos de drogas
Células Cultivadas
Citocinas/sangue
Citocinas/metabolismo
Relação Dose-Resposta a Droga
Antagonistas de Hormônios/farmacologia
Lactonas/farmacologia
Macrófagos Peritoneais/citologia
Camundongos Endogâmicos BALB C
Mifepristona/farmacologia
Oxido Nítrico/sangue
Extratos Vegetais/química
dc.subject.decs.pt_BR.fl_str_mv Lipopolissacarídeos/farmacologia
Ativação de Macrófagos/efeitos de drogas
Physalis/química
Fitosteróis/farmacologia
Sobrevivência Celular/efeitos de drogas
Células Cultivadas
Citocinas/sangue
Citocinas/metabolismo
Relação Dose-Resposta a Droga
Antagonistas de Hormônios/farmacologia
Lactonas/farmacologia
Macrófagos Peritoneais/citologia
Camundongos Endogâmicos BALB C
Mifepristona/farmacologia
Oxido Nítrico/sangue
Extratos Vegetais/química
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2012-07-18T18:18:01Z
dc.date.available.fl_str_mv 2012-07-18T18:18:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, M .B. et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 2003
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/4190
dc.identifier.issn.none.fl_str_mv 0014-2999
dc.identifier.other.none.fl_str_mv S0014-2999(02)02829-7
identifier_str_mv SOARES, M .B. et al. Inhibition of macrophage activation and lipopolysaccaride-induced death by seco-steroids purified from Physalis angulata L. European Journal of Pharmacology, v. 459, n. 1, p. 107-112, jan. 2003
0014-2999
S0014-2999(02)02829-7
url https://www.arca.fiocruz.br/handle/icict/4190
dc.language.iso.fl_str_mv eng
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institution FIOCRUZ
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