Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.

Silva, Suellen Leila Rocha; Santos, Luciano de Souza; Araujo Neto, João Honorato de; Dias, Rosane Borges; Sales, Caroline Brandi Schlaepfer; Rocha, Clarissa Araújo Gurgel; Soares, Milena Botelho Pereira; Batista, Alzir Azevedo; Bezerra, Daniel Pereira

Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.

Detalhes bibliográficos
Autor(a) principal:	Silva, Suellen Leila Rocha
Data de Publicação:	2019
Outros Autores:	Santos, Luciano de Souza, Araujo Neto, João Honorato de, Dias, Rosane Borges, Sales, Caroline Brandi Schlaepfer, Rocha, Clarissa Araújo Gurgel, Soares, Milena Botelho Pereira, Batista, Alzir Azevedo, Bezerra, Daniel Pereira
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/34698
Resumo:	Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).

Metadados do item

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network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Silva, Suellen Leila RochaSantos, Luciano de SouzaAraujo Neto, João Honorato deDias, Rosane BorgesSales, Caroline Brandi SchlaepferRocha, Clarissa Araújo GurgelSoares, Milena Botelho PereiraBatista, Alzir AzevedoBezerra, Daniel Pereira2019-08-09T18:02:53Z2019-08-09T18:02:53Z2019BALIZA, Ingrid Rayssa Souza et al. Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model. Frontiers in Oncology, v. 9, p. 1-11, 2019.2234-943Xhttps://www.arca.fiocruz.br/handle/icict/3469810.3389/fonc.2019.00582Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of São Carlos. Department of Chemistry. São Carlos, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Bahia. Institute of Health Sciences. Department of Biomorphology. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of São Carlos. Department of Chemistry. São Carlos, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Ruthenium complexes with piplartine, [Ru(piplartine)(dppf)(bipy)](PF6)2 (1) and [Ru(piplartine)(dppb)(bipy)](PF6)2 (2) (dppf = 1,1-bis(diphenylphosphino) ferrocene; dppb = 1,4-bis(diphenylphosphino)butane and bipy = 2,2'-bipyridine), were recently synthesized and displayed more potent cytotoxicity than piplartine in different cancer cells, regulated RNA transcripts of several apoptosis-related genes, and induced reactive oxygen species (ROS)-mediated apoptosis in human colon carcinoma HCT116 cells. The present work aimed to explore the underlying mechanisms through which these ruthenium complexes induce cell death in HCT116 cells in vitro, as well as their in vivo action in a xenograft model. Both complexes significantly increased the percentage of apoptotic HCT116 cells, and co-treatment with inhibitors of JNK/SAPK, p38 MAPK, and MEK, which inhibits the activation of ERK1/2, significantly reduced the apoptosis rate induced by these complexes. Moreover, significant increase in phospho-JNK2 (T183/Y185), phospho-p38α (T180/Y182), and phospho-ERK1 (T202/Y204) expressions were observed in cells treated with these complexes, indicating MAPK-mediated apoptosis. In addition, co-treatment with a p53 inhibitor (cyclic pifithrin-α) and the ruthenium complexes significantly reduced the apoptosis rate in HCT116 cells, and increased phospho-p53 (S15) and phospho-histone H2AX (S139) expressions, indicating induction of DNA damage and p53-dependent apoptosis. Both complexes also reduced HCT116 cell growth in a xenograft model. Tumor mass inhibition rates were 35.06, 29.71, and 32.03% for the complex 1 (15 μmol/kg/day), complex 2 (15 μmol/kg/day), and piplartine (60 μmol/kg/day), respectively. These data indicate these ruthenium complexes as new anti-colon cancer drugs candidates.engFrontiers MediaComplexos de rutênioPiplartinaPiperlonguminaApoptoseMAPKp53Ruthenium complexesPiplartinePiperlongumineApoptosisMAPKp53Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34698/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBaliza, I.R.S Ruthenium Complexs.pdfBaliza, I.R.S Ruthenium Complexs.pdfapplication/pdf4271774https://www.arca.fiocruz.br/bitstream/icict/34698/2/Baliza%2c%20I.R.S%20Ruthenium%20Complexs.pdf93dbef98611c8192c3a46387270a3f3dMD52TEXTBaliza, I.R.S Ruthenium Complexs.pdf.txtBaliza, I.R.S Ruthenium Complexs.pdf.txtExtracted texttext/plain45159https://www.arca.fiocruz.br/bitstream/icict/34698/3/Baliza%2c%20I.R.S%20Ruthenium%20Complexs.pdf.txtc7c284b6e6fd72b96fb8bf1858802e84MD53icict/346982023-03-15 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dc.title.pt_BR.fl_str_mv	Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.
title	Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.
spellingShingle	Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model. Silva, Suellen Leila Rocha Complexos de rutênio Piplartina Piperlongumina Apoptose MAPK p53 Ruthenium complexes Piplartine Piperlongumine Apoptosis MAPK p53
title_short	Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.
title_full	Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.
title_fullStr	Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.
title_full_unstemmed	Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.
title_sort	Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.
author	Silva, Suellen Leila Rocha
author_facet	Silva, Suellen Leila Rocha Santos, Luciano de Souza Araujo Neto, João Honorato de Dias, Rosane Borges Sales, Caroline Brandi Schlaepfer Rocha, Clarissa Araújo Gurgel Soares, Milena Botelho Pereira Batista, Alzir Azevedo Bezerra, Daniel Pereira
author_role	author
author2	Santos, Luciano de Souza Araujo Neto, João Honorato de Dias, Rosane Borges Sales, Caroline Brandi Schlaepfer Rocha, Clarissa Araújo Gurgel Soares, Milena Botelho Pereira Batista, Alzir Azevedo Bezerra, Daniel Pereira
author2_role	author author author author author author author author
dc.contributor.author.fl_str_mv	Silva, Suellen Leila Rocha Santos, Luciano de Souza Araujo Neto, João Honorato de Dias, Rosane Borges Sales, Caroline Brandi Schlaepfer Rocha, Clarissa Araújo Gurgel Soares, Milena Botelho Pereira Batista, Alzir Azevedo Bezerra, Daniel Pereira
dc.subject.other.pt_BR.fl_str_mv	Complexos de rutênio Piplartina Piperlongumina Apoptose MAPK p53
topic	Complexos de rutênio Piplartina Piperlongumina Apoptose MAPK p53 Ruthenium complexes Piplartine Piperlongumine Apoptosis MAPK p53
dc.subject.en.pt_BR.fl_str_mv	Ruthenium complexes Piplartine Piperlongumine Apoptosis MAPK p53
description	Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP).
publishDate	2019
dc.date.accessioned.fl_str_mv	2019-08-09T18:02:53Z
dc.date.available.fl_str_mv	2019-08-09T18:02:53Z
dc.date.issued.fl_str_mv	2019
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	BALIZA, Ingrid Rayssa Souza et al. Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model. Frontiers in Oncology, v. 9, p. 1-11, 2019.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/34698
dc.identifier.issn.pt_BR.fl_str_mv	2234-943X
dc.identifier.doi.none.fl_str_mv	10.3389/fonc.2019.00582
identifier_str_mv	BALIZA, Ingrid Rayssa Souza et al. Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model. Frontiers in Oncology, v. 9, p. 1-11, 2019. 2234-943X 10.3389/fonc.2019.00582
url	https://www.arca.fiocruz.br/handle/icict/34698
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Frontiers Media
publisher.none.fl_str_mv	Frontiers Media
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Ruthenium Complexes With Piplartine Cause Apoptosis Through MAPK Signaling by a p53-Dependent Pathway in Human Colon Carcinoma Cells and Inhibit Tumor Development in a Xenograft Model.

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