Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Parasitologia Veterinária (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400555 |
Resumo: | Abstract This study aimed to perform a morphological, molecular and phylogenetic characterization of Borrelia theileri obtained from infected Rhipicephalus microplus in Brazil. Fifty engorged R. microplus females from cattle in the municipality of Seropédica, Rio de Janeiro, were analyzed for spirochetes by hemolymph smear. Macerated eggs and positive ticks, as well as blood from the bovine infested by these ticks, were analyzed the glpQ, flaB and hpt genes by PCR. The PCR products were purified and sequenced for analysis and construction of a phylogenetic tree. Only 2% (1/50) of the ticks generated a positive result by both smear and PCR. The spiral forms (n = 50) had (media ± SD) a mean length of 19.17 ± 4.12 µm, diameter of 0.2935 ± 0.0469 and number of turns 8.44 ± 2.59. Sequence alignments of the three evaluated genes exhibited 98% similarity to B. theileri isolates, occurring in a clade highly related to B. theileri strain KAT. Egg maceration samples were positive for the three evaluated genes, whereas bovine blood was negative by PCR. This is the most detailed characterization of B. theileri in the Americas to-date, presenting morphological, molecular and phylogenetic data, including the transovarial transmission of the spirochete in the host tick. |
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Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplusRelapsing fever-likeBorreliaIxodidaephylogenetic characterizationAbstract This study aimed to perform a morphological, molecular and phylogenetic characterization of Borrelia theileri obtained from infected Rhipicephalus microplus in Brazil. Fifty engorged R. microplus females from cattle in the municipality of Seropédica, Rio de Janeiro, were analyzed for spirochetes by hemolymph smear. Macerated eggs and positive ticks, as well as blood from the bovine infested by these ticks, were analyzed the glpQ, flaB and hpt genes by PCR. The PCR products were purified and sequenced for analysis and construction of a phylogenetic tree. Only 2% (1/50) of the ticks generated a positive result by both smear and PCR. The spiral forms (n = 50) had (media ± SD) a mean length of 19.17 ± 4.12 µm, diameter of 0.2935 ± 0.0469 and number of turns 8.44 ± 2.59. Sequence alignments of the three evaluated genes exhibited 98% similarity to B. theileri isolates, occurring in a clade highly related to B. theileri strain KAT. Egg maceration samples were positive for the three evaluated genes, whereas bovine blood was negative by PCR. This is the most detailed characterization of B. theileri in the Americas to-date, presenting morphological, molecular and phylogenetic data, including the transovarial transmission of the spirochete in the host tick.Colégio Brasileiro de Parasitologia Veterinária2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400555Revista Brasileira de Parasitologia Veterinária v.27 n.4 2018reponame:Revista Brasileira de Parasitologia Veterinária (Online)instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV)instacron:CBPV10.1590/s1984-296120180083info:eu-repo/semantics/openAccessCordeiro,Matheus DiasBahia,MicheleMagalhães-Matos,Paulo CesarCepeda,Marcio BarizonGuterres,AlexandroFonseca,Adivaldo Henriqueeng2018-11-28T00:00:00Zoai:scielo:S1984-29612018000400555Revistahttp://www.scielo.br/scielo.php?script=sci_serial&lng=pt&pid=1984-2961https://old.scielo.br/oai/scielo-oai.php||zacariascbpv@fcav.unesp.br1984-29610103-846Xopendoar:2018-11-28T00:00Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV)false |
dc.title.none.fl_str_mv |
Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus |
title |
Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus |
spellingShingle |
Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus Cordeiro,Matheus Dias Relapsing fever-like Borrelia Ixodidae phylogenetic characterization |
title_short |
Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus |
title_full |
Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus |
title_fullStr |
Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus |
title_full_unstemmed |
Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus |
title_sort |
Morphological, molecular and phylogenetic characterization of Borrelia theileri in Rhipicephalus microplus |
author |
Cordeiro,Matheus Dias |
author_facet |
Cordeiro,Matheus Dias Bahia,Michele Magalhães-Matos,Paulo Cesar Cepeda,Marcio Barizon Guterres,Alexandro Fonseca,Adivaldo Henrique |
author_role |
author |
author2 |
Bahia,Michele Magalhães-Matos,Paulo Cesar Cepeda,Marcio Barizon Guterres,Alexandro Fonseca,Adivaldo Henrique |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cordeiro,Matheus Dias Bahia,Michele Magalhães-Matos,Paulo Cesar Cepeda,Marcio Barizon Guterres,Alexandro Fonseca,Adivaldo Henrique |
dc.subject.por.fl_str_mv |
Relapsing fever-like Borrelia Ixodidae phylogenetic characterization |
topic |
Relapsing fever-like Borrelia Ixodidae phylogenetic characterization |
description |
Abstract This study aimed to perform a morphological, molecular and phylogenetic characterization of Borrelia theileri obtained from infected Rhipicephalus microplus in Brazil. Fifty engorged R. microplus females from cattle in the municipality of Seropédica, Rio de Janeiro, were analyzed for spirochetes by hemolymph smear. Macerated eggs and positive ticks, as well as blood from the bovine infested by these ticks, were analyzed the glpQ, flaB and hpt genes by PCR. The PCR products were purified and sequenced for analysis and construction of a phylogenetic tree. Only 2% (1/50) of the ticks generated a positive result by both smear and PCR. The spiral forms (n = 50) had (media ± SD) a mean length of 19.17 ± 4.12 µm, diameter of 0.2935 ± 0.0469 and number of turns 8.44 ± 2.59. Sequence alignments of the three evaluated genes exhibited 98% similarity to B. theileri isolates, occurring in a clade highly related to B. theileri strain KAT. Egg maceration samples were positive for the three evaluated genes, whereas bovine blood was negative by PCR. This is the most detailed characterization of B. theileri in the Americas to-date, presenting morphological, molecular and phylogenetic data, including the transovarial transmission of the spirochete in the host tick. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400555 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400555 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1984-296120180083 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária v.27 n.4 2018 reponame:Revista Brasileira de Parasitologia Veterinária (Online) instname:Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
instname_str |
Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
instacron_str |
CBPV |
institution |
CBPV |
reponame_str |
Revista Brasileira de Parasitologia Veterinária (Online) |
collection |
Revista Brasileira de Parasitologia Veterinária (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária (Online) - Colégio Brasileiro de Parasitologia Veterinária (CBPV) |
repository.mail.fl_str_mv |
||zacariascbpv@fcav.unesp.br |
_version_ |
1754208917112487936 |