Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex

Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG Brasil

Access type:openAccess
Publication Date:2015
Main Author: Soares, Marco Aurélio
Other Authors: Araújo, Roberta Amália de Carvalho, Marini, Marjorie Mendes, Oliveira, Luciana Márcia de, Lima, Leonardo Gomes de, Alves, Viviane de Souza, Felipe, Maria Sueli Soares, Brigido, Marcelo Macedo, Soares, Celia Maria de Almeida, Silveira, Jose Franco da, Ruiz, Jeronimo Conceição, Cisalpino, Patrícia Silva
Document type: Article
Language:eng
Published: BioMed Central
Online Access:https://www.arca.fiocruz.br/handle/icict/14487
Citation:SOARES, Marco Aurélio et al. Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex. BMC Genomics, vol. 16, n. 1, p. 376, 2015.
English abstract:BACKGROUND: Species from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transposons in Paracoccidioides genomes. In this work, we aimed to identify potentially active retrotransposons in Paracoccidioides genomes. RESULTS: We identified five different retrotransposon families (four LTR-like and one LINE-like element) in the genomes of three Paracoccidioides isolates. Retrotransposons were present in all of the genomes analyzed. P. brasiliensis and P. lutzii species harbored the same retrotransposon lineages but differed in their copy numbers. In the Pb01, Pb03 and Pb18 genomes, the number of LTR retrotransposons was higher than the number of LINE-like elements, and the LINE-like element RtPc5 was transcribed in Paracoccidioides lutzii (Pb01) but could not be detected in P. brasiliensis (Pb03 and Pb18) by semi-quantitative RT-PCR. CONCLUSION: Five new potentially active retrotransposons have been identified in the genomic assemblies of the Paracoccidioides species complex using a combined computational and experimental approach. The distribution across the two known species, P. brasiliensis and P. lutzii, and phylogenetics analysis indicate that these elements could have been acquired before speciation occurred. The presence of active retrotransposons in the genome may have implications regarding the evolution and genetic diversification of the Paracoccidioides genus.