Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex

Detalhes bibliográficos
Autor(a) principal: Soares, Marco Aurélio
Data de Publicação: 2015
Outros Autores: Araújo, Roberta Amália de Carvalho, Marini, Marjorie Mendes, Oliveira, Luciana Márcia de, Lima, Leonardo Gomes de, Alves, Viviane de Souza, Felipe, Maria Sueli Soares, Brigido, Marcelo Macedo, Soares, Celia Maria de Almeida, Silveira, Jose Franco da, Ruiz, Jeronimo Conceição, Cisalpino, Patrícia Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14487
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG Brasil.
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spelling Soares, Marco AurélioAraújo, Roberta Amália de CarvalhoMarini, Marjorie MendesOliveira, Luciana Márcia deLima, Leonardo Gomes deAlves, Viviane de SouzaFelipe, Maria Sueli SoaresBrigido, Marcelo MacedoSoares, Celia Maria de AlmeidaSilveira, Jose Franco daRuiz, Jeronimo ConceiçãoCisalpino, Patrícia Silva2016-06-16T18:44:03Z2016-07-08T02:09:55Z2016-06-16T18:44:03Z2016-07-08T02:09:55Z2015SOARES, Marco Aurélio et al. Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex. BMC Genomics, v. 16, n. 1, p. 1-15, 2015.1471-2164https://www.arca.fiocruz.br/handle/icict/1448710.1186/s12864-015-1564-7engBioMed CentralIdentification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complexinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Programa de Pós-graduação em Bioinformática. Belo Horizonte, MG Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Informática de Biossistema. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Biologia Geral. Belo Horizonte, MG, Brasil.Universidade de Brasília. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Brasília, DF, Brasil.Universidade de Brasília. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Brasília, DF, Brasil.Universidade de Brasília. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Brasília, DF, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Informática de Biossistema. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Programa de Pós-graduação em Bioinformática. Belo Horizonte, MG, Brasil.BACKGROUND: Species from the Paracoccidioides complex are thermally dimorphic fungi and the causative agents of paracoccidioidomycosis, a deep fungal infection that is the most prevalent systemic mycosis in Latin America and represents the most important cause of death in immunocompetent individuals with systemic mycosis in Brazil. We previously described the identification of eight new families of DNA transposons in Paracoccidioides genomes. In this work, we aimed to identify potentially active retrotransposons in Paracoccidioides genomes. RESULTS: We identified five different retrotransposon families (four LTR-like and one LINE-like element) in the genomes of three Paracoccidioides isolates. Retrotransposons were present in all of the genomes analyzed. P. brasiliensis and P. lutzii species harbored the same retrotransposon lineages but differed in their copy numbers. In the Pb01, Pb03 and Pb18 genomes, the number of LTR retrotransposons was higher than the number of LINE-like elements, and the LINE-like element RtPc5 was transcribed in Paracoccidioides lutzii (Pb01) but could not be detected in P. brasiliensis (Pb03 and Pb18) by semi-quantitative RT-PCR. CONCLUSION: Five new potentially active retrotransposons have been identified in the genomic assemblies of the Paracoccidioides species complex using a combined computational and experimental approach. The distribution across the two known species, P. brasiliensis and P. lutzii, and phylogenetics analysis indicate that these elements could have been acquired before speciation occurred. The presence of active retrotransposons in the genome may have implications regarding the evolution and genetic diversification of the Paracoccidioides genus.Expressed retrotransposonsGenomeParacoccidioidesParacoccidioides species complexinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTve_Marco_Soares_etal_Identification_CPqRR_2015.pdf.txtve_Marco_Soares_etal_Identification_CPqRR_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
title Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
spellingShingle Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
Soares, Marco Aurélio
Expressed retrotransposons
Genome
Paracoccidioides
Paracoccidioides species complex
title_short Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
title_full Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
title_fullStr Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
title_full_unstemmed Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
title_sort Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex
author Soares, Marco Aurélio
author_facet Soares, Marco Aurélio
Araújo, Roberta Amália de Carvalho
Marini, Marjorie Mendes
Oliveira, Luciana Márcia de
Lima, Leonardo Gomes de
Alves, Viviane de Souza
Felipe, Maria Sueli Soares
Brigido, Marcelo Macedo
Soares, Celia Maria de Almeida
Silveira, Jose Franco da
Ruiz, Jeronimo Conceição
Cisalpino, Patrícia Silva
author_role author
author2 Araújo, Roberta Amália de Carvalho
Marini, Marjorie Mendes
Oliveira, Luciana Márcia de
Lima, Leonardo Gomes de
Alves, Viviane de Souza
Felipe, Maria Sueli Soares
Brigido, Marcelo Macedo
Soares, Celia Maria de Almeida
Silveira, Jose Franco da
Ruiz, Jeronimo Conceição
Cisalpino, Patrícia Silva
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Soares, Marco Aurélio
Araújo, Roberta Amália de Carvalho
Marini, Marjorie Mendes
Oliveira, Luciana Márcia de
Lima, Leonardo Gomes de
Alves, Viviane de Souza
Felipe, Maria Sueli Soares
Brigido, Marcelo Macedo
Soares, Celia Maria de Almeida
Silveira, Jose Franco da
Ruiz, Jeronimo Conceição
Cisalpino, Patrícia Silva
dc.subject.en.pt_BR.fl_str_mv Expressed retrotransposons
Genome
Paracoccidioides
Paracoccidioides species complex
topic Expressed retrotransposons
Genome
Paracoccidioides
Paracoccidioides species complex
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-06-16T18:44:03Z
2016-07-08T02:09:55Z
dc.date.available.fl_str_mv 2016-06-16T18:44:03Z
2016-07-08T02:09:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, Marco Aurélio et al. Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex. BMC Genomics, v. 16, n. 1, p. 1-15, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14487
dc.identifier.issn.pt_BR.fl_str_mv 1471-2164
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/s12864-015-1564-7
identifier_str_mv SOARES, Marco Aurélio et al. Identification and characterization of expressed retrotransposons in the genome of the Paracoccidioides species complex. BMC Genomics, v. 16, n. 1, p. 1-15, 2015.
1471-2164
10.1186/s12864-015-1564-7
url https://www.arca.fiocruz.br/handle/icict/14487
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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