Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000500431 |
Resumo: | Abstract: The present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. |
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Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicusFish hematologyblood clottingEDTAHeparinPiaractus mesopotamicus.Abstract: The present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood.Colégio Brasileiro de Patologia Animal - CBPA2016-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000500431Pesquisa Veterinária Brasileira v.36 n.5 2016reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2016000500013info:eu-repo/semantics/openAccessFarias,Thaís Heloisa VazPereira,Nycolas LevyPádua,Santiago Benites deAlves,Lindomar de OliveiraSakabe,RóbersonBelo,Marco Antonio de AndradePilarski,Fabianaeng2016-07-25T00:00:00Zoai:scielo:S0100-736X2016000500431Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2016-07-25T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus |
title |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus |
spellingShingle |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus Farias,Thaís Heloisa Vaz Fish hematology blood clotting EDTA Heparin Piaractus mesopotamicus. |
title_short |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus |
title_full |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus |
title_fullStr |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus |
title_full_unstemmed |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus |
title_sort |
Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus |
author |
Farias,Thaís Heloisa Vaz |
author_facet |
Farias,Thaís Heloisa Vaz Pereira,Nycolas Levy Pádua,Santiago Benites de Alves,Lindomar de Oliveira Sakabe,Róberson Belo,Marco Antonio de Andrade Pilarski,Fabiana |
author_role |
author |
author2 |
Pereira,Nycolas Levy Pádua,Santiago Benites de Alves,Lindomar de Oliveira Sakabe,Róberson Belo,Marco Antonio de Andrade Pilarski,Fabiana |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Farias,Thaís Heloisa Vaz Pereira,Nycolas Levy Pádua,Santiago Benites de Alves,Lindomar de Oliveira Sakabe,Róberson Belo,Marco Antonio de Andrade Pilarski,Fabiana |
dc.subject.por.fl_str_mv |
Fish hematology blood clotting EDTA Heparin Piaractus mesopotamicus. |
topic |
Fish hematology blood clotting EDTA Heparin Piaractus mesopotamicus. |
description |
Abstract: The present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000500431 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000500431 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2016000500013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.36 n.5 2016 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122236013314048 |