Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5

Detalhes bibliográficos
Autor(a) principal: Holz,Carine Lidiane
Data de Publicação: 2010
Outros Autores: Cibulski,Samuel Paulo, Teixeira,Thais Fumaco, Batista,Helena Beatriz C.R., Dezen,Diógenes, Campos,Fabrício Souza, Varela,Ana Paula M., Roehe,Paulo Michel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010000700001
Resumo: The serum neutralization (SN) test is the gold standard method to measure neutralizing antibodies to bovine herpesviruses. However, in view of the further subdivisions of bovine herpesviruses in types/subtypes, defining which virus to use at challenge in SN tests may be difficult. In view of that, this study was carried out to re-evaluate (SN) sensitivity with different types/subtypes of bovine herpesviruses types 1 (BoHV-1) and 5 (BoHV-5) as challenge viruses. Bovine sera (n=810) were collected from two distinct geographic regions and tested by SN with three type 1 viruses (BoHV-1.1 strains "Los Angeles" and "EVI123/98"; BoHV-1.2a strain "SV265/96") and three type 5 viruses (BoHV-5a strain "EVI88/95"; BoHV-5b strain "A663" and BoHV-5c "ISO97/95"). SN tests were performed with a 1 hour incubation of the serum-virus mixtures at 37ºC against 100 TCID50 of each of the viruses. SN sensitivity varied greatly depending on the challenge virus used in the test. The highest sensitivity (327 positive/810 total sera tested; 40.37%) was attained when the positive results to the six viruses were added together. No association could be found between any particular type or subtype of virus and the sensitivity of the test. When positive results to each single strain were considered, SN sensitivity varied from 41.7% to 81.7%, depending on the virus and the geographic region of origin of the sera. Variation was detected even when challenge viruses belonged to the same subtype, where disagreement between positive results reached 41%. These results indicate that one hour incubation SN tests against single viruses, as performed here, may display a significantly low sensitivity (p=0.05); performing SN tests against a number of different viruses may increase considerably SN sensitivity. Furthermore, the choice of virus used for challenge is critical in SN tests. In addition, sera from different geographic regions may give rise to disagreeing results with different strains of BoHV-1 and BoHV-5. This might be particularly relevant for control programs and in international trade, were maximum sensitivity should be targeted.
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spelling Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5Neutralizationcross-neutralizationBoHV-1BoHV-5The serum neutralization (SN) test is the gold standard method to measure neutralizing antibodies to bovine herpesviruses. However, in view of the further subdivisions of bovine herpesviruses in types/subtypes, defining which virus to use at challenge in SN tests may be difficult. In view of that, this study was carried out to re-evaluate (SN) sensitivity with different types/subtypes of bovine herpesviruses types 1 (BoHV-1) and 5 (BoHV-5) as challenge viruses. Bovine sera (n=810) were collected from two distinct geographic regions and tested by SN with three type 1 viruses (BoHV-1.1 strains "Los Angeles" and "EVI123/98"; BoHV-1.2a strain "SV265/96") and three type 5 viruses (BoHV-5a strain "EVI88/95"; BoHV-5b strain "A663" and BoHV-5c "ISO97/95"). SN tests were performed with a 1 hour incubation of the serum-virus mixtures at 37ºC against 100 TCID50 of each of the viruses. SN sensitivity varied greatly depending on the challenge virus used in the test. The highest sensitivity (327 positive/810 total sera tested; 40.37%) was attained when the positive results to the six viruses were added together. No association could be found between any particular type or subtype of virus and the sensitivity of the test. When positive results to each single strain were considered, SN sensitivity varied from 41.7% to 81.7%, depending on the virus and the geographic region of origin of the sera. Variation was detected even when challenge viruses belonged to the same subtype, where disagreement between positive results reached 41%. These results indicate that one hour incubation SN tests against single viruses, as performed here, may display a significantly low sensitivity (p=0.05); performing SN tests against a number of different viruses may increase considerably SN sensitivity. Furthermore, the choice of virus used for challenge is critical in SN tests. In addition, sera from different geographic regions may give rise to disagreeing results with different strains of BoHV-1 and BoHV-5. This might be particularly relevant for control programs and in international trade, were maximum sensitivity should be targeted.Colégio Brasileiro de Patologia Animal - CBPA2010-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010000700001Pesquisa Veterinária Brasileira v.30 n.7 2010reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2010000700001info:eu-repo/semantics/openAccessHolz,Carine LidianeCibulski,Samuel PauloTeixeira,Thais FumacoBatista,Helena Beatriz C.R.Dezen,DiógenesCampos,Fabrício SouzaVarela,Ana Paula M.Roehe,Paulo Micheleng2010-08-30T00:00:00Zoai:scielo:S0100-736X2010000700001Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2010-08-30T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
title Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
spellingShingle Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
Holz,Carine Lidiane
Neutralization
cross-neutralization
BoHV-1
BoHV-5
title_short Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
title_full Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
title_fullStr Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
title_full_unstemmed Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
title_sort Serum neutralization with different types and subtypes of bovine herpesvirus 1 and 5
author Holz,Carine Lidiane
author_facet Holz,Carine Lidiane
Cibulski,Samuel Paulo
Teixeira,Thais Fumaco
Batista,Helena Beatriz C.R.
Dezen,Diógenes
Campos,Fabrício Souza
Varela,Ana Paula M.
Roehe,Paulo Michel
author_role author
author2 Cibulski,Samuel Paulo
Teixeira,Thais Fumaco
Batista,Helena Beatriz C.R.
Dezen,Diógenes
Campos,Fabrício Souza
Varela,Ana Paula M.
Roehe,Paulo Michel
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Holz,Carine Lidiane
Cibulski,Samuel Paulo
Teixeira,Thais Fumaco
Batista,Helena Beatriz C.R.
Dezen,Diógenes
Campos,Fabrício Souza
Varela,Ana Paula M.
Roehe,Paulo Michel
dc.subject.por.fl_str_mv Neutralization
cross-neutralization
BoHV-1
BoHV-5
topic Neutralization
cross-neutralization
BoHV-1
BoHV-5
description The serum neutralization (SN) test is the gold standard method to measure neutralizing antibodies to bovine herpesviruses. However, in view of the further subdivisions of bovine herpesviruses in types/subtypes, defining which virus to use at challenge in SN tests may be difficult. In view of that, this study was carried out to re-evaluate (SN) sensitivity with different types/subtypes of bovine herpesviruses types 1 (BoHV-1) and 5 (BoHV-5) as challenge viruses. Bovine sera (n=810) were collected from two distinct geographic regions and tested by SN with three type 1 viruses (BoHV-1.1 strains "Los Angeles" and "EVI123/98"; BoHV-1.2a strain "SV265/96") and three type 5 viruses (BoHV-5a strain "EVI88/95"; BoHV-5b strain "A663" and BoHV-5c "ISO97/95"). SN tests were performed with a 1 hour incubation of the serum-virus mixtures at 37ºC against 100 TCID50 of each of the viruses. SN sensitivity varied greatly depending on the challenge virus used in the test. The highest sensitivity (327 positive/810 total sera tested; 40.37%) was attained when the positive results to the six viruses were added together. No association could be found between any particular type or subtype of virus and the sensitivity of the test. When positive results to each single strain were considered, SN sensitivity varied from 41.7% to 81.7%, depending on the virus and the geographic region of origin of the sera. Variation was detected even when challenge viruses belonged to the same subtype, where disagreement between positive results reached 41%. These results indicate that one hour incubation SN tests against single viruses, as performed here, may display a significantly low sensitivity (p=0.05); performing SN tests against a number of different viruses may increase considerably SN sensitivity. Furthermore, the choice of virus used for challenge is critical in SN tests. In addition, sera from different geographic regions may give rise to disagreeing results with different strains of BoHV-1 and BoHV-5. This might be particularly relevant for control programs and in international trade, were maximum sensitivity should be targeted.
publishDate 2010
dc.date.none.fl_str_mv 2010-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010000700001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010000700001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2010000700001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.30 n.7 2010
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
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institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
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repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
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