Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/10483 |
Resumo: | The objective of this work was to investigate the effect of dietary supplementation with essential fatty acids on the kinetics of macrophage accumulation and giant cell formation in Nile tilapia (Oreochromis niloticus). The supplementation sources were soybean oil (SO, source of omega 6, n‑6) and linseed oil (LO, source of omega 3, n‑3), in the following proportions: 100% SO; 75% SO + 25% LO; 50% SO + 50% LO; 25% SO + 75% LO; and 100% LO (four replicates per treatment). After a feeding period of three months, growth performance was evaluated, and glass coverslips were implanted into the subcutaneous connective tissue of fish, being removed for examination at 2, 4, 6, and 8 days after implantation. Growth performance did not differ between treatments. Fish fed 100% linseed oil diet had the greatest macrophage accumulation and the fastest Langhans cell formation on the sixth day. On the eighth day, Langhans cells were predominant on the coverslips implanted in the fish feed 75 and 100% linseed oil. n‑3 fatty acids may contribute to macrophage recruitment and giant cell formation in fish chronic inflammatory response to foreign body. |
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Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acidsCinética da inflamação crônica em tilápia‑do‑nilo alimentada com ácidos graxos essenciais n‑3 e n‑6Oreochromis niloticus; foreign body; growth performance; inflammatory response; lipid nutritionOreochromis niloticus; corpo estranho; desempenho produtivo; resposta inflamatória; nutrição lipídicaThe objective of this work was to investigate the effect of dietary supplementation with essential fatty acids on the kinetics of macrophage accumulation and giant cell formation in Nile tilapia (Oreochromis niloticus). The supplementation sources were soybean oil (SO, source of omega 6, n‑6) and linseed oil (LO, source of omega 3, n‑3), in the following proportions: 100% SO; 75% SO + 25% LO; 50% SO + 50% LO; 25% SO + 75% LO; and 100% LO (four replicates per treatment). After a feeding period of three months, growth performance was evaluated, and glass coverslips were implanted into the subcutaneous connective tissue of fish, being removed for examination at 2, 4, 6, and 8 days after implantation. Growth performance did not differ between treatments. Fish fed 100% linseed oil diet had the greatest macrophage accumulation and the fastest Langhans cell formation on the sixth day. On the eighth day, Langhans cells were predominant on the coverslips implanted in the fish feed 75 and 100% linseed oil. n‑3 fatty acids may contribute to macrophage recruitment and giant cell formation in fish chronic inflammatory response to foreign body.O objetivo deste trabalho foi investigar o efeito da suplementação alimentar com ácidos graxos essenciais sobre a cinética do acúmulo de macrófagos e a formação de células gigantes em tilápia‑do‑nilo (Oreochromis niloticus). As fontes de suplementação foram óleo de soja (OS, fonte de ômega 6, n‑6) e óleo de linhaça (OL, fonte de ômega 3, n‑3), nas seguintes proporções: 100% OS; 75% OS + 25% OL; 50% OS + 50% OL; 25% OS + 75% OL; e 100% OL (quatro repetições por tratamento). Após período de alimentação de três meses, foi avaliado o desempenho produtivo, e lamínulas de vidro foram implantadas no tecido subcutâneo dos peixes, as quais foram removidas para exame aos 2, 4, 6 e 8 dias após o implante. O desempenho produtivo não diferiu entre os tratamentos. Os peixes alimentados com 100% de óleo de linhaça tiveram maior acúmulo de macrófagos e formação mais rápida de células de Langhans, no sexto dia. No oitavo dia, as células de Langhans foram predominantes nas lamínulas implantadas nos peixes alimentados com 75 e 100% de óleo de linhaça. Os ácidos graxos n‑3 podem contribuir para o recrutamento de macrófagos e a formação de células gigantes, na resposta inflamatória crônica a corpo estranho em peixes.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFapesp, processo: 06/55065-6Sakabe, RóbersonMoraes, Flávio Ruas deBelo, Marco Antonio de AndradePilarski, FabianaMoraes, Julieta Rodini Engrácia de2013-06-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/10483Pesquisa Agropecuaria Brasileira; v.48, n.3, mar. 2013; 313-319Pesquisa Agropecuária Brasileira; v.48, n.3, mar. 2013; 313-3191678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/10483/11130https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10483/5938info:eu-repo/semantics/openAccess2013-06-06T21:18:09Zoai:ojs.seer.sct.embrapa.br:article/10483Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2013-06-06T21:18:09Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids Cinética da inflamação crônica em tilápia‑do‑nilo alimentada com ácidos graxos essenciais n‑3 e n‑6 |
title |
Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids |
spellingShingle |
Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids Sakabe, Róberson Oreochromis niloticus; foreign body; growth performance; inflammatory response; lipid nutrition Oreochromis niloticus; corpo estranho; desempenho produtivo; resposta inflamatória; nutrição lipídica |
title_short |
Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids |
title_full |
Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids |
title_fullStr |
Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids |
title_full_unstemmed |
Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids |
title_sort |
Kinetics of chronic inflammation in Nile tilapia fed n‑3 and n‑6 essential fatty acids |
author |
Sakabe, Róberson |
author_facet |
Sakabe, Róberson Moraes, Flávio Ruas de Belo, Marco Antonio de Andrade Pilarski, Fabiana Moraes, Julieta Rodini Engrácia de |
author_role |
author |
author2 |
Moraes, Flávio Ruas de Belo, Marco Antonio de Andrade Pilarski, Fabiana Moraes, Julieta Rodini Engrácia de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Fapesp, processo: 06/55065-6 |
dc.contributor.author.fl_str_mv |
Sakabe, Róberson Moraes, Flávio Ruas de Belo, Marco Antonio de Andrade Pilarski, Fabiana Moraes, Julieta Rodini Engrácia de |
dc.subject.por.fl_str_mv |
Oreochromis niloticus; foreign body; growth performance; inflammatory response; lipid nutrition Oreochromis niloticus; corpo estranho; desempenho produtivo; resposta inflamatória; nutrição lipídica |
topic |
Oreochromis niloticus; foreign body; growth performance; inflammatory response; lipid nutrition Oreochromis niloticus; corpo estranho; desempenho produtivo; resposta inflamatória; nutrição lipídica |
description |
The objective of this work was to investigate the effect of dietary supplementation with essential fatty acids on the kinetics of macrophage accumulation and giant cell formation in Nile tilapia (Oreochromis niloticus). The supplementation sources were soybean oil (SO, source of omega 6, n‑6) and linseed oil (LO, source of omega 3, n‑3), in the following proportions: 100% SO; 75% SO + 25% LO; 50% SO + 50% LO; 25% SO + 75% LO; and 100% LO (four replicates per treatment). After a feeding period of three months, growth performance was evaluated, and glass coverslips were implanted into the subcutaneous connective tissue of fish, being removed for examination at 2, 4, 6, and 8 days after implantation. Growth performance did not differ between treatments. Fish fed 100% linseed oil diet had the greatest macrophage accumulation and the fastest Langhans cell formation on the sixth day. On the eighth day, Langhans cells were predominant on the coverslips implanted in the fish feed 75 and 100% linseed oil. n‑3 fatty acids may contribute to macrophage recruitment and giant cell formation in fish chronic inflammatory response to foreign body. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-06 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/10483 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/10483 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/10483/11130 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10483/5938 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.48, n.3, mar. 2013; 313-319 Pesquisa Agropecuária Brasileira; v.48, n.3, mar. 2013; 313-319 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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