Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.

Detalhes bibliográficos
Autor(a) principal: QUINTERO, F. O. C.
Data de Publicação: 2018
Outros Autores: PINTO, L. G., BARSALOBRES-CAVALLARI, C. F., ARCURI, M. de L. C., PINO, L. E., PERES, L. E. P., MALUF, M. P., MAIA, I. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113247
Resumo: In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.
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spelling Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.TomatoPromoterSeed maturation proteinSeed-specific expressionCoffeeCaféIn this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.Fabíola O. Campo Quintero, Department of Genetics/Institute of Biosciences/Sao Paulo State University - UNESP; Layra G. Pinto, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Carla F. Barsalobres-Cavallari, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Mariana de Lara Campos Arcuri, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Lilian Ellen Pino, Department of Biological Sciences - LCB/Escola Superior de Agricultura 'Luiz de Queiroz' - ESALQ/University of São Paulo - USP; Lázaro Eustáquio Pereira Peres, Department of Biological Sciences - LCB/Escola Superior de Agricultura 'Luiz de Queiroz' - ESALQ/University of São Paulo - USP; MIRIAN PEREZ MALUF, CNPCa; Ivan G. Maia, Department of Genetics/Institute of Biosciences/Sao Paulo State University - UNESP.QUINTERO, F. O. C.PINTO, L. G.BARSALOBRES-CAVALLARI, C. F.ARCURI, M. de L. C.PINO, L. E.PERES, L. E. P.MALUF, M. P.MAIA, I. G.2019-10-21T18:04:28Z2019-10-21T18:04:28Z2019-10-2120182019-10-21T18:04:28Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Cell Reports, v. 37, n.9, p.1257–1268, September 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113247enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-10-21T18:04:36Zoai:www.alice.cnptia.embrapa.br:doc/1113247Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-10-21T18:04:36falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-10-21T18:04:36Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
title Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
spellingShingle Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
QUINTERO, F. O. C.
Tomato
Promoter
Seed maturation protein
Seed-specific expression
Coffee
Café
title_short Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
title_full Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
title_fullStr Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
title_full_unstemmed Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
title_sort Identification of a seed maturation protein gene from Coffea arabica (CaSMP) and analysis of its promoter activity in tomato.
author QUINTERO, F. O. C.
author_facet QUINTERO, F. O. C.
PINTO, L. G.
BARSALOBRES-CAVALLARI, C. F.
ARCURI, M. de L. C.
PINO, L. E.
PERES, L. E. P.
MALUF, M. P.
MAIA, I. G.
author_role author
author2 PINTO, L. G.
BARSALOBRES-CAVALLARI, C. F.
ARCURI, M. de L. C.
PINO, L. E.
PERES, L. E. P.
MALUF, M. P.
MAIA, I. G.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fabíola O. Campo Quintero, Department of Genetics/Institute of Biosciences/Sao Paulo State University - UNESP; Layra G. Pinto, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Carla F. Barsalobres-Cavallari, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Mariana de Lara Campos Arcuri, Department of Genetics/Institute of Biosciences/São Paulo State University - UNESP; Lilian Ellen Pino, Department of Biological Sciences - LCB/Escola Superior de Agricultura 'Luiz de Queiroz' - ESALQ/University of São Paulo - USP; Lázaro Eustáquio Pereira Peres, Department of Biological Sciences - LCB/Escola Superior de Agricultura 'Luiz de Queiroz' - ESALQ/University of São Paulo - USP; MIRIAN PEREZ MALUF, CNPCa; Ivan G. Maia, Department of Genetics/Institute of Biosciences/Sao Paulo State University - UNESP.
dc.contributor.author.fl_str_mv QUINTERO, F. O. C.
PINTO, L. G.
BARSALOBRES-CAVALLARI, C. F.
ARCURI, M. de L. C.
PINO, L. E.
PERES, L. E. P.
MALUF, M. P.
MAIA, I. G.
dc.subject.por.fl_str_mv Tomato
Promoter
Seed maturation protein
Seed-specific expression
Coffee
Café
topic Tomato
Promoter
Seed maturation protein
Seed-specific expression
Coffee
Café
description In this report, an expressed sequence tag-based approach was used to identify a seed-specific candidate gene for promoter isolation in Coffea arabica. The tissue-specific expression of the cognate gene (CaSMP), which encodes a yet uncharacterized coffee seed maturation protein, was validated by RT-qPCR. Additional expression analysis during coffee fruit development revealed higher levels of CaSMP transcript accumulation in the yellow/green phenological stage. Moreover, CaSMP was preferentially expressed in the endosperm and was down-regulated during water imbibition of the seeds. The presence of regulatory cis-elements known to be involved in seed- and endosperm-specific expression was observed in the CaSMP 5?-upstream region amplified by genome walking (GW). Additional histochemical analysis of transgenic tomato (cv. Micro-Tom) lines harboring the GW-amplified fragment (~ 1.4 kb) fused to uidA reporter gene confirmed promoter activity in the ovule of immature tomato fruits, while no activity was observed in the seeds of ripening fruits and in the other organs/tissues examined. These results indicate that the CaSMP promoter can be used to drive transgene expression in coffee beans and tomato seeds, thus representing a promising biotechnological tool.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-10-21T18:04:28Z
2019-10-21T18:04:28Z
2019-10-21
2019-10-21T18:04:28Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plant Cell Reports, v. 37, n.9, p.1257–1268, September 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113247
identifier_str_mv Plant Cell Reports, v. 37, n.9, p.1257–1268, September 2018.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113247
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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