Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/953013 |
Resumo: | Ingestion of proteinase inhibitors leads to hyperproduction of digestive proteinases, limiting the bioavailability of essential amino acids for protein synthesis, which affects insect growth and development. However, the effects of proteinase inhibitors on digestive enzymes can lead to an adaptive response by the insect. In here, we assessed the biochemical response of midgut proteinases from the eucalypt defoliator Thyrinteina arnobia (Stoll) to different concentrations of berenil, a bisbenzamidine proteinase inhibitor, on eucalyptus. Eucalyptus leaves were immersed in berenil solutions at different concentrations and fed to larvae of T. arnobia. Mortality was assessed daily. The proteolytic activity in the midgut of T. arnobia was assessed after feeding on plants sprayed with aqueous solutions of berenil, fed to fifth instars of T. arnobia for 48 h before midgut removal for enzymatic assays. Larvae of T. arnobia were able to overcome the effects of the lowest berenil concentrations by increasing their trypsin-like activity, but not as berenil concentration increased, despite the fact that the highest berenil concentration resulted in overproduction of trypsin-like proteinases. Berenil also prevented the increase of the cysteine proteinases activity in response to trypsin inhibition. |
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Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor.Synthetic inhibitorPest controltrypsinIngestion of proteinase inhibitors leads to hyperproduction of digestive proteinases, limiting the bioavailability of essential amino acids for protein synthesis, which affects insect growth and development. However, the effects of proteinase inhibitors on digestive enzymes can lead to an adaptive response by the insect. In here, we assessed the biochemical response of midgut proteinases from the eucalypt defoliator Thyrinteina arnobia (Stoll) to different concentrations of berenil, a bisbenzamidine proteinase inhibitor, on eucalyptus. Eucalyptus leaves were immersed in berenil solutions at different concentrations and fed to larvae of T. arnobia. Mortality was assessed daily. The proteolytic activity in the midgut of T. arnobia was assessed after feeding on plants sprayed with aqueous solutions of berenil, fed to fifth instars of T. arnobia for 48 h before midgut removal for enzymatic assays. Larvae of T. arnobia were able to overcome the effects of the lowest berenil concentrations by increasing their trypsin-like activity, but not as berenil concentration increased, despite the fact that the highest berenil concentration resulted in overproduction of trypsin-like proteinases. Berenil also prevented the increase of the cysteine proteinases activity in response to trypsin inhibition.JEANNE SCARDINI MARINHO PRADO, CNPMA; A. L. LOURENÇÃO, IAC; R. N. C. GUEDES, Departamento de Entomologia - UFV; A. PALLINI, Departamento de Entomologia - UFV; J. A. OLIVEIRA, Departamento de Química - UFV; M. G. A. OLIVEIRA, Departamento de Bioquímica e Biologia Molecular - UFV.PRADO, J. S. M.LOURENÇÃO, A. L.GUEDES, R. N. C.PALLINI, A.OLIVEIRA, J. A.OLIVEIRA, M. G. A.2013-03-14T11:11:11Z2013-03-14T11:11:11Z2013-03-1420122013-03-14T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNeotropical Entomology, v. 41, p. 420-425, 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/953013enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:19:40Zoai:www.alice.cnptia.embrapa.br:doc/953013Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:19:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:19:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. |
title |
Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. |
spellingShingle |
Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. PRADO, J. S. M. Synthetic inhibitor Pest control trypsin |
title_short |
Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. |
title_full |
Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. |
title_fullStr |
Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. |
title_full_unstemmed |
Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. |
title_sort |
Enzymatic response of the eucalypt defoliator Thyrinteina arnobia (Stoll) (Lepidoptera: Geometridae) to a bis-benzamidine proteinase inhibitor. |
author |
PRADO, J. S. M. |
author_facet |
PRADO, J. S. M. LOURENÇÃO, A. L. GUEDES, R. N. C. PALLINI, A. OLIVEIRA, J. A. OLIVEIRA, M. G. A. |
author_role |
author |
author2 |
LOURENÇÃO, A. L. GUEDES, R. N. C. PALLINI, A. OLIVEIRA, J. A. OLIVEIRA, M. G. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
JEANNE SCARDINI MARINHO PRADO, CNPMA; A. L. LOURENÇÃO, IAC; R. N. C. GUEDES, Departamento de Entomologia - UFV; A. PALLINI, Departamento de Entomologia - UFV; J. A. OLIVEIRA, Departamento de Química - UFV; M. G. A. OLIVEIRA, Departamento de Bioquímica e Biologia Molecular - UFV. |
dc.contributor.author.fl_str_mv |
PRADO, J. S. M. LOURENÇÃO, A. L. GUEDES, R. N. C. PALLINI, A. OLIVEIRA, J. A. OLIVEIRA, M. G. A. |
dc.subject.por.fl_str_mv |
Synthetic inhibitor Pest control trypsin |
topic |
Synthetic inhibitor Pest control trypsin |
description |
Ingestion of proteinase inhibitors leads to hyperproduction of digestive proteinases, limiting the bioavailability of essential amino acids for protein synthesis, which affects insect growth and development. However, the effects of proteinase inhibitors on digestive enzymes can lead to an adaptive response by the insect. In here, we assessed the biochemical response of midgut proteinases from the eucalypt defoliator Thyrinteina arnobia (Stoll) to different concentrations of berenil, a bisbenzamidine proteinase inhibitor, on eucalyptus. Eucalyptus leaves were immersed in berenil solutions at different concentrations and fed to larvae of T. arnobia. Mortality was assessed daily. The proteolytic activity in the midgut of T. arnobia was assessed after feeding on plants sprayed with aqueous solutions of berenil, fed to fifth instars of T. arnobia for 48 h before midgut removal for enzymatic assays. Larvae of T. arnobia were able to overcome the effects of the lowest berenil concentrations by increasing their trypsin-like activity, but not as berenil concentration increased, despite the fact that the highest berenil concentration resulted in overproduction of trypsin-like proteinases. Berenil also prevented the increase of the cysteine proteinases activity in response to trypsin inhibition. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2013-03-14T11:11:11Z 2013-03-14T11:11:11Z 2013-03-14 2013-03-14T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Neotropical Entomology, v. 41, p. 420-425, 2012. http://www.alice.cnptia.embrapa.br/alice/handle/doc/953013 |
identifier_str_mv |
Neotropical Entomology, v. 41, p. 420-425, 2012. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/953013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503376366469120 |