Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061878 |
Resumo: | The Dof (DNA-binding with one finger) protein family spans a group of plant transcription factors involved in the regulation of several functions, such as plant responses to stress, hormones and light, phytochrome signaling and seed germination. Here we describe the Dof-like gene family in grapevine (Vitis vinifera L.), which consists of 25 genes coding for Dof. An extensive in silico characterization of the VviDofL gene family was performed. Additionally, the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray (cv Corvina) and real-time PCR (cv Pinot Noir) analyses. The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis, tomato, poplar and already described Dof genes in other species allowed us to identify several duplicated genes. The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles. Furthermore, distinct expression patterns were identified between samples analyzed, corroborating such hypothesis. Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower, berry and seed development, highlighting their importance for grapevine growth and production. The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir. |
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Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development.VideiraPinot NoirDesenvolvimento da sementeBerryGrapevineDiversification of grapevineFlowerUvaGenéticaGeneConservaçãoCorvinaSeed developmentThe Dof (DNA-binding with one finger) protein family spans a group of plant transcription factors involved in the regulation of several functions, such as plant responses to stress, hormones and light, phytochrome signaling and seed germination. Here we describe the Dof-like gene family in grapevine (Vitis vinifera L.), which consists of 25 genes coding for Dof. An extensive in silico characterization of the VviDofL gene family was performed. Additionally, the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray (cv Corvina) and real-time PCR (cv Pinot Noir) analyses. The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis, tomato, poplar and already described Dof genes in other species allowed us to identify several duplicated genes. The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles. Furthermore, distinct expression patterns were identified between samples analyzed, corroborating such hypothesis. Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower, berry and seed development, highlighting their importance for grapevine growth and production. The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir.DANIELLE COSTENARO DA SILVA, Universidade Federal do Rio Grande do Sul; VÍTOR DA SILVEIRA FALAVIGNA, Universidade Federal do Rio Grande do Sul; MARIANNA FASOLI, Dipartimento di Biotecnologie, Università degli Studi di Verona; VANESSA BUFFON, CNPUV; DIOGO DENARDI PORTO, CPATSA; GEORGIOS JOANNIS PAPPAS JÚNIOR, UnB; MARIO PEZZOTTI, Dipartimento di Biotecnologie, Università degli Studi di Verona; GIANCARLO PASQUALI, Universidade Federal do Rio Grande do Sul; LUIS FERNANDO REVERS, CNPUV.SILVA, D. C. daFALAVIGNA, V. da S.FASOLI, M.BUFFON, V.PORTO, D. D.PAPPAS JÚNIOR, G. J.PEZZOTTI, M.PASQUALI, G.REVERS, L. F.2017-01-26T11:11:11Z2017-01-26T11:11:11Z2017-01-2620162017-04-25T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHorticulture Research, v. 3, p. 1-10, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/106187810.1038/hortres.2016.42enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:05:54Zoai:www.alice.cnptia.embrapa.br:doc/1061878Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:05:54falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:05:54Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. |
title |
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. |
spellingShingle |
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. SILVA, D. C. da Videira Pinot Noir Desenvolvimento da semente Berry Grapevine Diversification of grapevine Flower Uva Genética Gene Conservação Corvina Seed development |
title_short |
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. |
title_full |
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. |
title_fullStr |
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. |
title_full_unstemmed |
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. |
title_sort |
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development. |
author |
SILVA, D. C. da |
author_facet |
SILVA, D. C. da FALAVIGNA, V. da S. FASOLI, M. BUFFON, V. PORTO, D. D. PAPPAS JÚNIOR, G. J. PEZZOTTI, M. PASQUALI, G. REVERS, L. F. |
author_role |
author |
author2 |
FALAVIGNA, V. da S. FASOLI, M. BUFFON, V. PORTO, D. D. PAPPAS JÚNIOR, G. J. PEZZOTTI, M. PASQUALI, G. REVERS, L. F. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
DANIELLE COSTENARO DA SILVA, Universidade Federal do Rio Grande do Sul; VÍTOR DA SILVEIRA FALAVIGNA, Universidade Federal do Rio Grande do Sul; MARIANNA FASOLI, Dipartimento di Biotecnologie, Università degli Studi di Verona; VANESSA BUFFON, CNPUV; DIOGO DENARDI PORTO, CPATSA; GEORGIOS JOANNIS PAPPAS JÚNIOR, UnB; MARIO PEZZOTTI, Dipartimento di Biotecnologie, Università degli Studi di Verona; GIANCARLO PASQUALI, Universidade Federal do Rio Grande do Sul; LUIS FERNANDO REVERS, CNPUV. |
dc.contributor.author.fl_str_mv |
SILVA, D. C. da FALAVIGNA, V. da S. FASOLI, M. BUFFON, V. PORTO, D. D. PAPPAS JÚNIOR, G. J. PEZZOTTI, M. PASQUALI, G. REVERS, L. F. |
dc.subject.por.fl_str_mv |
Videira Pinot Noir Desenvolvimento da semente Berry Grapevine Diversification of grapevine Flower Uva Genética Gene Conservação Corvina Seed development |
topic |
Videira Pinot Noir Desenvolvimento da semente Berry Grapevine Diversification of grapevine Flower Uva Genética Gene Conservação Corvina Seed development |
description |
The Dof (DNA-binding with one finger) protein family spans a group of plant transcription factors involved in the regulation of several functions, such as plant responses to stress, hormones and light, phytochrome signaling and seed germination. Here we describe the Dof-like gene family in grapevine (Vitis vinifera L.), which consists of 25 genes coding for Dof. An extensive in silico characterization of the VviDofL gene family was performed. Additionally, the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray (cv Corvina) and real-time PCR (cv Pinot Noir) analyses. The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis, tomato, poplar and already described Dof genes in other species allowed us to identify several duplicated genes. The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles. Furthermore, distinct expression patterns were identified between samples analyzed, corroborating such hypothesis. Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower, berry and seed development, highlighting their importance for grapevine growth and production. The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-01-26T11:11:11Z 2017-01-26T11:11:11Z 2017-01-26 2017-04-25T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Horticulture Research, v. 3, p. 1-10, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061878 10.1038/hortres.2016.42 |
identifier_str_mv |
Horticulture Research, v. 3, p. 1-10, 2016. 10.1038/hortres.2016.42 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061878 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503431755399168 |