Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114229 https://doi.org/10.1155/2019/3562672 |
Resumo: | The immune response against Haemonchus contortus infections is primarily associated with the Th2 profile. However, the exact mechanisms associated with increased sheep resistance against this parasite remains poorly elucidated. The present study is aimed at evaluating mediators from the innate immune response in lambs of the Morada Nova Brazilian breed with contrasting H. contortus resistance phenotypes. Briefly, 287 lambs were characterized through fecal egg counts (FEC) and packed cell volume (PCV) after two independent experimental parasitic challenges with 4,000 H. contortus L3. 20 extreme resistance phenotypes (10 most resistant and 10 most susceptible) were selected, subjected to a third artificial infection with 4,000 L3, and euthanized 7 days later. Tissue samples were collected from abomasal fundic and pyloric mucosa and abomasal lymph nodes. Blood samples were collected at days 0 and 7 of the third parasitic challenge. RNA was extracted from tissue and blood samples for relative quantification of innate immune-related genes by RT-qPCR. For the abomasal fundic mucosa, increased TNFα and IL1β expression levels (P < 0:05) were found in the susceptible animals, while resistant animals had IL33 superiorly expressed (P < 0:05). Higher levels (P < 0:05) of TLR2 and CFI were found in the abomasal pyloric mucosa of resistant animals. TNFα was at higher levels (P < 0:05) in the blood of susceptible lambs, at day 0 of the third artificial infection. The exacerbated proinflammatory response observed in susceptible animals, at both local and systemic levels, may be a consequence of high H. contortus parasitism. This hypothesis is corroborated by the higher blood levels of TNFα before the onset of infection, which probably remained elevated from the previous parasitic challenges. On the other hand, resistant lambs had an enhanced response mediated by TLR recognition and complement activation. Nevertheless, this is the first study to directly associate sheep parasitic resistance with IL33, an innate trigger of the Th2-polarized response. |
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Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep.Sheep resistanceFecal egg countsGastrointestinal nematodeOvinoLambsAnimal parasites and pestsThe immune response against Haemonchus contortus infections is primarily associated with the Th2 profile. However, the exact mechanisms associated with increased sheep resistance against this parasite remains poorly elucidated. The present study is aimed at evaluating mediators from the innate immune response in lambs of the Morada Nova Brazilian breed with contrasting H. contortus resistance phenotypes. Briefly, 287 lambs were characterized through fecal egg counts (FEC) and packed cell volume (PCV) after two independent experimental parasitic challenges with 4,000 H. contortus L3. 20 extreme resistance phenotypes (10 most resistant and 10 most susceptible) were selected, subjected to a third artificial infection with 4,000 L3, and euthanized 7 days later. Tissue samples were collected from abomasal fundic and pyloric mucosa and abomasal lymph nodes. Blood samples were collected at days 0 and 7 of the third parasitic challenge. RNA was extracted from tissue and blood samples for relative quantification of innate immune-related genes by RT-qPCR. For the abomasal fundic mucosa, increased TNFα and IL1β expression levels (P < 0:05) were found in the susceptible animals, while resistant animals had IL33 superiorly expressed (P < 0:05). Higher levels (P < 0:05) of TLR2 and CFI were found in the abomasal pyloric mucosa of resistant animals. TNFα was at higher levels (P < 0:05) in the blood of susceptible lambs, at day 0 of the third artificial infection. The exacerbated proinflammatory response observed in susceptible animals, at both local and systemic levels, may be a consequence of high H. contortus parasitism. This hypothesis is corroborated by the higher blood levels of TNFα before the onset of infection, which probably remained elevated from the previous parasitic challenges. On the other hand, resistant lambs had an enhanced response mediated by TLR recognition and complement activation. Nevertheless, this is the first study to directly associate sheep parasitic resistance with IL33, an innate trigger of the Th2-polarized response.JOÃO HENRIQUE BARBOSA TOSCANO, UNESPCINTIA HIROMI OKINO, CPPSEISABELLA BARBOSA DOS SANTOS, UNESPLUCIANA APARECIDA GIRALDELO, UNICEPMAREI BORSCH VON HAEHLING, UNESPSERGIO NOVITA ESTEVES, CPPSEANA CAROLINA DE SOUZA CHAGAS, CPPSE.TOSCANO, J. H. B.OKINO, C. H.SANTOS, I. B. dosGIRALDELO, L. A.HAEHLING, M. B. VONESTEVES, S. N.CHAGAS, A. C. de S.2019-11-13T00:43:46Z2019-11-13T00:43:46Z2019-11-1220192019-11-21T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Immunology Research, v. 2019, n. 3562672, p. 1-10, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114229https://doi.org/10.1155/2019/3562672enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-11-13T00:43:53Zoai:www.alice.cnptia.embrapa.br:doc/1114229Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-11-13T00:43:53falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-11-13T00:43:53Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. |
title |
Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. |
spellingShingle |
Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. TOSCANO, J. H. B. Sheep resistance Fecal egg counts Gastrointestinal nematode Ovino Lambs Animal parasites and pests |
title_short |
Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. |
title_full |
Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. |
title_fullStr |
Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. |
title_full_unstemmed |
Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. |
title_sort |
Innate immune responses associated with resistance against Haemonchus Contortus in Morada Nova sheep. |
author |
TOSCANO, J. H. B. |
author_facet |
TOSCANO, J. H. B. OKINO, C. H. SANTOS, I. B. dos GIRALDELO, L. A. HAEHLING, M. B. VON ESTEVES, S. N. CHAGAS, A. C. de S. |
author_role |
author |
author2 |
OKINO, C. H. SANTOS, I. B. dos GIRALDELO, L. A. HAEHLING, M. B. VON ESTEVES, S. N. CHAGAS, A. C. de S. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
JOÃO HENRIQUE BARBOSA TOSCANO, UNESP CINTIA HIROMI OKINO, CPPSE ISABELLA BARBOSA DOS SANTOS, UNESP LUCIANA APARECIDA GIRALDELO, UNICEP MAREI BORSCH VON HAEHLING, UNESP SERGIO NOVITA ESTEVES, CPPSE ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
dc.contributor.author.fl_str_mv |
TOSCANO, J. H. B. OKINO, C. H. SANTOS, I. B. dos GIRALDELO, L. A. HAEHLING, M. B. VON ESTEVES, S. N. CHAGAS, A. C. de S. |
dc.subject.por.fl_str_mv |
Sheep resistance Fecal egg counts Gastrointestinal nematode Ovino Lambs Animal parasites and pests |
topic |
Sheep resistance Fecal egg counts Gastrointestinal nematode Ovino Lambs Animal parasites and pests |
description |
The immune response against Haemonchus contortus infections is primarily associated with the Th2 profile. However, the exact mechanisms associated with increased sheep resistance against this parasite remains poorly elucidated. The present study is aimed at evaluating mediators from the innate immune response in lambs of the Morada Nova Brazilian breed with contrasting H. contortus resistance phenotypes. Briefly, 287 lambs were characterized through fecal egg counts (FEC) and packed cell volume (PCV) after two independent experimental parasitic challenges with 4,000 H. contortus L3. 20 extreme resistance phenotypes (10 most resistant and 10 most susceptible) were selected, subjected to a third artificial infection with 4,000 L3, and euthanized 7 days later. Tissue samples were collected from abomasal fundic and pyloric mucosa and abomasal lymph nodes. Blood samples were collected at days 0 and 7 of the third parasitic challenge. RNA was extracted from tissue and blood samples for relative quantification of innate immune-related genes by RT-qPCR. For the abomasal fundic mucosa, increased TNFα and IL1β expression levels (P < 0:05) were found in the susceptible animals, while resistant animals had IL33 superiorly expressed (P < 0:05). Higher levels (P < 0:05) of TLR2 and CFI were found in the abomasal pyloric mucosa of resistant animals. TNFα was at higher levels (P < 0:05) in the blood of susceptible lambs, at day 0 of the third artificial infection. The exacerbated proinflammatory response observed in susceptible animals, at both local and systemic levels, may be a consequence of high H. contortus parasitism. This hypothesis is corroborated by the higher blood levels of TNFα before the onset of infection, which probably remained elevated from the previous parasitic challenges. On the other hand, resistant lambs had an enhanced response mediated by TLR recognition and complement activation. Nevertheless, this is the first study to directly associate sheep parasitic resistance with IL33, an innate trigger of the Th2-polarized response. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-13T00:43:46Z 2019-11-13T00:43:46Z 2019-11-12 2019 2019-11-21T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Immunology Research, v. 2019, n. 3562672, p. 1-10, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114229 https://doi.org/10.1155/2019/3562672 |
identifier_str_mv |
Journal of Immunology Research, v. 2019, n. 3562672, p. 1-10, 2019. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114229 https://doi.org/10.1155/2019/3562672 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503483841314816 |