Discrimination of common bean cultivars using multiplexed microsatellite markers.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085520 |
Resumo: | Analysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not different from zero (F-ST = 0.01). The molecular profile database derived from these analyses may increase the statistical power of genetic estimates and may be incorporated into breeding programs for common bean. Furthermore, the profiles obtained for the different cultivars may be used as molecular descriptors to complement traditional descriptors used in distinctiveness, uniformity and stability tests, thereby improving the traceability of samples and their derivatives and helping to protect the intellectual property rights of breeders. |
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Discrimination of common bean cultivars using multiplexed microsatellite markers.Diversidade genéticaFeijãoPhaseolus vulgarisDNAMarcador molecularAnalysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not different from zero (F-ST = 0.01). The molecular profile database derived from these analyses may increase the statistical power of genetic estimates and may be incorporated into breeding programs for common bean. Furthermore, the profiles obtained for the different cultivars may be used as molecular descriptors to complement traditional descriptors used in distinctiveness, uniformity and stability tests, thereby improving the traceability of samples and their derivatives and helping to protect the intellectual property rights of breeders.P. C. B. CARDOSO; CLAUDIO BRONDANI, CNPAF; I. P. P. MENEZES; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.CARDOSO, P. C. B.BRONDANI, C.MENEZES, I. P. P.VALDISSER, P. A. M. R.BORBA, T. C. O.DEL PELOSO, M. J.VIANELLO, R. P.2018-01-16T23:22:02Z2018-01-16T23:22:02Z2018-01-1620142018-01-16T23:22:02Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 13, n. 1, p. 1964-1978, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/108552010.4238/2014.March.24.1enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-01-16T23:22:09Zoai:www.alice.cnptia.embrapa.br:doc/1085520Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-01-16T23:22:09falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-01-16T23:22:09Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
title |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
spellingShingle |
Discrimination of common bean cultivars using multiplexed microsatellite markers. CARDOSO, P. C. B. Diversidade genética Feijão Phaseolus vulgaris DNA Marcador molecular |
title_short |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
title_full |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
title_fullStr |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
title_full_unstemmed |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
title_sort |
Discrimination of common bean cultivars using multiplexed microsatellite markers. |
author |
CARDOSO, P. C. B. |
author_facet |
CARDOSO, P. C. B. BRONDANI, C. MENEZES, I. P. P. VALDISSER, P. A. M. R. BORBA, T. C. O. DEL PELOSO, M. J. VIANELLO, R. P. |
author_role |
author |
author2 |
BRONDANI, C. MENEZES, I. P. P. VALDISSER, P. A. M. R. BORBA, T. C. O. DEL PELOSO, M. J. VIANELLO, R. P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
P. C. B. CARDOSO; CLAUDIO BRONDANI, CNPAF; I. P. P. MENEZES; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
dc.contributor.author.fl_str_mv |
CARDOSO, P. C. B. BRONDANI, C. MENEZES, I. P. P. VALDISSER, P. A. M. R. BORBA, T. C. O. DEL PELOSO, M. J. VIANELLO, R. P. |
dc.subject.por.fl_str_mv |
Diversidade genética Feijão Phaseolus vulgaris DNA Marcador molecular |
topic |
Diversidade genética Feijão Phaseolus vulgaris DNA Marcador molecular |
description |
Analysis of DNA polymorphisms allows for the genetic identification and precise discrimination of species with a narrow genetic base such as common bean. The primary objectives of the present study were to molecularly characterize commercial common bean varieties developed at various research institutions using microsatellite markers and to determine the degree of genetic diversity among the bean varieties analyzed. Fifty cultivars representing 12 grain classes and 64 genitors, i.e., accessions used to develop these cultivars, were characterized. Based on an analysis of 24 simple sequence repeats, the estimates for the average number of alleles and genetic diversity were 8.29 and 0.646, respectively. The combined probability of identity was estimated at 7.05 x 10(-17), indicating a high individual discriminatory power. Thirty-two percent of the cultivars exhibited heterogeneity for multiple loci that reflected either homozygosity for different alleles of a given locus in different individuals or heterozygosity for the locus. The average genetic diversity for the groups of cultivars and genitors was 0.605 and 0.660, respectively, with no genetic differentiation (F-ST) between these groups. Although similar estimates of expected heterozygosity were observed when the cultivars were grouped by release date, a greater number of private alleles was observed in the most recent cultivars. The genetic differentiation among cultivars originating from different institutions was not different from zero (F-ST = 0.01). The molecular profile database derived from these analyses may increase the statistical power of genetic estimates and may be incorporated into breeding programs for common bean. Furthermore, the profiles obtained for the different cultivars may be used as molecular descriptors to complement traditional descriptors used in distinctiveness, uniformity and stability tests, thereby improving the traceability of samples and their derivatives and helping to protect the intellectual property rights of breeders. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2018-01-16T23:22:02Z 2018-01-16T23:22:02Z 2018-01-16 2018-01-16T23:22:02Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 13, n. 1, p. 1964-1978, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085520 10.4238/2014.March.24.1 |
identifier_str_mv |
Genetics and Molecular Research, v. 13, n. 1, p. 1964-1978, 2014. 10.4238/2014.March.24.1 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085520 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503448211750912 |