Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.

Detalhes bibliográficos
Autor(a) principal: BARROS, A. T. M. de
Data de Publicação: 2013
Outros Autores: SCHUMAKER, T. T. S., KOLLER, W. W., KLAFKE, G. M., ALBUQUERQUE, T. A. de, GONZALEZ, R.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/970876
Resumo: Horn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ? 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity.
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spelling Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.Horn flyKdrMetabolic resistanceMosca-dos-chifresResistência a inseticidasResistência metabólicainsecticide resistanceHorn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ? 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity.2013-11-08T11:11:11Z2013-11-08T11:11:11Z2013-11-0820132013-11-08T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Parasitologia Veterinária v. 22, n. 1, p. 136-142, 2013.0103-846Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/970876porBARROS, A. T. M. deSCHUMAKER, T. T. S.KOLLER, W. W.KLAFKE, G. M.ALBUQUERQUE, T. A. deGONZALEZ, R.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T00:35:48Zoai:www.alice.cnptia.embrapa.br:doc/970876Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T00:35:48falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T00:35:48Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.
title Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.
spellingShingle Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.
BARROS, A. T. M. de
Horn fly
Kdr
Metabolic resistance
Mosca-dos-chifres
Resistência a inseticidas
Resistência metabólica
insecticide resistance
title_short Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.
title_full Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.
title_fullStr Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.
title_full_unstemmed Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.
title_sort Mechanisms of pyrethroid resistance in Haematobia irritans (Muscidae) from Mato Grosso do Sul state, Brazil.
author BARROS, A. T. M. de
author_facet BARROS, A. T. M. de
SCHUMAKER, T. T. S.
KOLLER, W. W.
KLAFKE, G. M.
ALBUQUERQUE, T. A. de
GONZALEZ, R.
author_role author
author2 SCHUMAKER, T. T. S.
KOLLER, W. W.
KLAFKE, G. M.
ALBUQUERQUE, T. A. de
GONZALEZ, R.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv BARROS, A. T. M. de
SCHUMAKER, T. T. S.
KOLLER, W. W.
KLAFKE, G. M.
ALBUQUERQUE, T. A. de
GONZALEZ, R.
dc.subject.por.fl_str_mv Horn fly
Kdr
Metabolic resistance
Mosca-dos-chifres
Resistência a inseticidas
Resistência metabólica
insecticide resistance
topic Horn fly
Kdr
Metabolic resistance
Mosca-dos-chifres
Resistência a inseticidas
Resistência metabólica
insecticide resistance
description Horn fly resistance to pyrethroid insecticides occurs throughout Brazil, but knowledge about the involved mechanisms is still in an incipient stage. This survey was aimed to identify the mechanisms of horn fly resistance to cypermethrin in Mato Grosso do Sul state, Brazil. Impregnated filter paper bioassays using cypermethrin, synergized or not with piperonyl butoxide (PBO) and triphenyl phosphate (TPP), were conducted from March 2004 to June 2005 in horn fly populations (n = 33) from all over the state. All populations were highly resistant to cypermethrin, with resistance factors (RF) ranging from 89.4 to 1,020.6. Polymerase chain reaction (PCR) assays to detect the knockdown resistance (kdr) mutation also were performed in 16 samples. The kdr mutation was found in 75% of the tested populations, mostly with relatively low frequencies (<20%), and was absent in some highly resistant populations. Addition of TPP did not significantly reduce the LC50 in any population. However, PBO reduced LC50s above 40-fold in all tested populations, resulting in RFs ? 10 in most cases. Horn fly resistance to cypermethrin is widespread in the state, being primarily caused by an enhanced activity of P450 mono-oxygenases and secondarily by reduced target site sensitivity.
publishDate 2013
dc.date.none.fl_str_mv 2013-11-08T11:11:11Z
2013-11-08T11:11:11Z
2013-11-08
2013
2013-11-08T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
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format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Parasitologia Veterinária v. 22, n. 1, p. 136-142, 2013.
0103-846X
http://www.alice.cnptia.embrapa.br/alice/handle/doc/970876
identifier_str_mv Revista Brasileira de Parasitologia Veterinária v. 22, n. 1, p. 136-142, 2013.
0103-846X
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/970876
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instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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