New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061159 |
Resumo: | The Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help Taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to perform molecular characterization of ticks in all five regions of Brazil and infer their phylogenetic relationships. Molecular analysis characterized 10 haplotypes of the COX-1 gene. Molecular network analysis revealed that haplotype H-2 was the most dispersed of the studied populations (n = 11). Haplotype H-3 (n = 2) had the greatest genetic differentiation when compared to other Brazilian populations. A Bayesian phylogenetic tree of the COX-1 gene obtained strong support. In addition, it was observed that the population of R. (B.) microplushaplotype H-3 exhibited diverging branches among the other Brazilian populations in the study. The study concludes that the different regions of Brazil have R. (B.) microplus tick populations with distinct haplotypes. |
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New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil.COX-I geneITS-2 geneCarrapato-do-boiRhipicephalus microplusTicksBrazilThe Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help Taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to perform molecular characterization of ticks in all five regions of Brazil and infer their phylogenetic relationships. Molecular analysis characterized 10 haplotypes of the COX-1 gene. Molecular network analysis revealed that haplotype H-2 was the most dispersed of the studied populations (n = 11). Haplotype H-3 (n = 2) had the greatest genetic differentiation when compared to other Brazilian populations. A Bayesian phylogenetic tree of the COX-1 gene obtained strong support. In addition, it was observed that the population of R. (B.) microplushaplotype H-3 exhibited diverging branches among the other Brazilian populations in the study. The study concludes that the different regions of Brazil have R. (B.) microplus tick populations with distinct haplotypes.BÁRBARA GUIMARÃES CSORDAS, UFMS; MARCOS VALÉRIO GARCIA, Bolsista CNPGC; RODRIGO CASQUERO CUNHA, UFPEL; POLIANA FERNANDA GIACHETTO, CNPTIA; ISABELLA MAIUMI ZAIDAN BLECHA, UFMS; RENATO ANDREOTTI E SILVA, CNPGC.CSORDAS, B. G.GARCIA, M. V.CUNHA, R. C.GIACHETTO, P. F.BLECHA, I. M. Z.ANDREOTTI, R.2017-01-18T11:11:11Z2017-01-18T11:11:11Z2017-01-1820162017-03-02T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Parasitologia Veterinaria , Jaboticabal, v. 25, n. 3, p. 317-326, jul./set. 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061159enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:04:08Zoai:www.alice.cnptia.embrapa.br:doc/1061159Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:04:08falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:04:08Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil. |
title |
New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil. |
spellingShingle |
New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil. CSORDAS, B. G. COX-I gene ITS-2 gene Carrapato-do-boi Rhipicephalus microplus Ticks Brazil |
title_short |
New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil. |
title_full |
New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil. |
title_fullStr |
New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil. |
title_full_unstemmed |
New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil. |
title_sort |
New insights from molecular characterization of the tick Rhipicephalus (Bophilus) microplus in Brazil. |
author |
CSORDAS, B. G. |
author_facet |
CSORDAS, B. G. GARCIA, M. V. CUNHA, R. C. GIACHETTO, P. F. BLECHA, I. M. Z. ANDREOTTI, R. |
author_role |
author |
author2 |
GARCIA, M. V. CUNHA, R. C. GIACHETTO, P. F. BLECHA, I. M. Z. ANDREOTTI, R. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
BÁRBARA GUIMARÃES CSORDAS, UFMS; MARCOS VALÉRIO GARCIA, Bolsista CNPGC; RODRIGO CASQUERO CUNHA, UFPEL; POLIANA FERNANDA GIACHETTO, CNPTIA; ISABELLA MAIUMI ZAIDAN BLECHA, UFMS; RENATO ANDREOTTI E SILVA, CNPGC. |
dc.contributor.author.fl_str_mv |
CSORDAS, B. G. GARCIA, M. V. CUNHA, R. C. GIACHETTO, P. F. BLECHA, I. M. Z. ANDREOTTI, R. |
dc.subject.por.fl_str_mv |
COX-I gene ITS-2 gene Carrapato-do-boi Rhipicephalus microplus Ticks Brazil |
topic |
COX-I gene ITS-2 gene Carrapato-do-boi Rhipicephalus microplus Ticks Brazil |
description |
The Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help Taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to perform molecular characterization of ticks in all five regions of Brazil and infer their phylogenetic relationships. Molecular analysis characterized 10 haplotypes of the COX-1 gene. Molecular network analysis revealed that haplotype H-2 was the most dispersed of the studied populations (n = 11). Haplotype H-3 (n = 2) had the greatest genetic differentiation when compared to other Brazilian populations. A Bayesian phylogenetic tree of the COX-1 gene obtained strong support. In addition, it was observed that the population of R. (B.) microplushaplotype H-3 exhibited diverging branches among the other Brazilian populations in the study. The study concludes that the different regions of Brazil have R. (B.) microplus tick populations with distinct haplotypes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-01-18T11:11:11Z 2017-01-18T11:11:11Z 2017-01-18 2017-03-02T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria , Jaboticabal, v. 25, n. 3, p. 317-326, jul./set. 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061159 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria , Jaboticabal, v. 25, n. 3, p. 317-326, jul./set. 2016. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061159 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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