Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin

Detalhes bibliográficos
Autor(a) principal: Barth,O. M.
Data de Publicação: 1988
Outros Autores: Majerowicz,S., Menasce,L. P., Schatzmayr,H. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000200010
Resumo: The presence of viral antigen in sections from formalin-fixed and paraffin-embedded human tissues was demonstrated by trypsin digestion followed by direct or indirect immunofluorescence. The specimens may be used for retrospective diagnosis. The immunofluorescence technique has to be adapted to the suspected virus infection on the basis of previous histopathology study. Variations of trypsin concentration time and temperature of incubation, expose different viral antigens and have to be previously tested for each unknown system. For measles virus detection in lung a stronger digestion has to be applied as compared to adenovirus or respiratory disease viruses in the same tisue. Flavivirus in liver tissue needs a weaker digestion. The reproducibility of the method makes it useful as a routine technique in diagnosis of virus infection.
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spelling Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsinvirus diagnosisimmunofluorescenceretrospective diagnosistrypsin digestionThe presence of viral antigen in sections from formalin-fixed and paraffin-embedded human tissues was demonstrated by trypsin digestion followed by direct or indirect immunofluorescence. The specimens may be used for retrospective diagnosis. The immunofluorescence technique has to be adapted to the suspected virus infection on the basis of previous histopathology study. Variations of trypsin concentration time and temperature of incubation, expose different viral antigens and have to be previously tested for each unknown system. For measles virus detection in lung a stronger digestion has to be applied as compared to adenovirus or respiratory disease viruses in the same tisue. Flavivirus in liver tissue needs a weaker digestion. The reproducibility of the method makes it useful as a routine technique in diagnosis of virus infection.Instituto Oswaldo Cruz, Ministério da Saúde1988-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000200010Memórias do Instituto Oswaldo Cruz v.83 n.2 1988reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761988000200010info:eu-repo/semantics/openAccessBarth,O. M.Majerowicz,S.Menasce,L. P.Schatzmayr,H. G.eng2020-04-25T17:46:06Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:23.916Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
title Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
spellingShingle Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
Barth,O. M.
virus diagnosis
immunofluorescence
retrospective diagnosis
trypsin digestion
title_short Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
title_full Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
title_fullStr Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
title_full_unstemmed Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
title_sort Detection of viral infection by immunofluorescence in formalin-fixed tissues, pretreated with trypsin
author Barth,O. M.
author_facet Barth,O. M.
Majerowicz,S.
Menasce,L. P.
Schatzmayr,H. G.
author_role author
author2 Majerowicz,S.
Menasce,L. P.
Schatzmayr,H. G.
author2_role author
author
author
dc.contributor.author.fl_str_mv Barth,O. M.
Majerowicz,S.
Menasce,L. P.
Schatzmayr,H. G.
dc.subject.por.fl_str_mv virus diagnosis
immunofluorescence
retrospective diagnosis
trypsin digestion
topic virus diagnosis
immunofluorescence
retrospective diagnosis
trypsin digestion
dc.description.none.fl_txt_mv The presence of viral antigen in sections from formalin-fixed and paraffin-embedded human tissues was demonstrated by trypsin digestion followed by direct or indirect immunofluorescence. The specimens may be used for retrospective diagnosis. The immunofluorescence technique has to be adapted to the suspected virus infection on the basis of previous histopathology study. Variations of trypsin concentration time and temperature of incubation, expose different viral antigens and have to be previously tested for each unknown system. For measles virus detection in lung a stronger digestion has to be applied as compared to adenovirus or respiratory disease viruses in the same tisue. Flavivirus in liver tissue needs a weaker digestion. The reproducibility of the method makes it useful as a routine technique in diagnosis of virus infection.
description The presence of viral antigen in sections from formalin-fixed and paraffin-embedded human tissues was demonstrated by trypsin digestion followed by direct or indirect immunofluorescence. The specimens may be used for retrospective diagnosis. The immunofluorescence technique has to be adapted to the suspected virus infection on the basis of previous histopathology study. Variations of trypsin concentration time and temperature of incubation, expose different viral antigens and have to be previously tested for each unknown system. For measles virus detection in lung a stronger digestion has to be applied as compared to adenovirus or respiratory disease viruses in the same tisue. Flavivirus in liver tissue needs a weaker digestion. The reproducibility of the method makes it useful as a routine technique in diagnosis of virus infection.
publishDate 1988
dc.date.none.fl_str_mv 1988-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000200010
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000200010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761988000200010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.83 n.2 1988
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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