Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)

Detalhes bibliográficos
Autor(a) principal: Souza,Ligia Cristina Kalb
Data de Publicação: 2013
Outros Autores: Pinho,Rosana Elisa Gonçalves Gonçalves, Lima,Carla Vanessa de Paula, Fragoso,Stênio Perdigão, Soares,Maurilio José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500631
Resumo: Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.
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spelling Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)actinconfocal microscopytrypanosomatidsTrypanosoma cruziHeteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.Instituto Oswaldo Cruz, Ministério da Saúde2013-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500631Memórias do Instituto Oswaldo Cruz v.108 n.5 2013reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/0074-0276108052013015info:eu-repo/semantics/openAccessSouza,Ligia Cristina KalbPinho,Rosana Elisa Gonçalves GonçalvesLima,Carla Vanessa de PaulaFragoso,Stênio PerdigãoSoares,Maurilio Joséeng2020-04-25T17:51:34Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:19:07.348Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
spellingShingle Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
Souza,Ligia Cristina Kalb
actin
confocal microscopy
trypanosomatids
Trypanosoma cruzi
title_short Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title_full Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title_fullStr Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title_full_unstemmed Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title_sort Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
author Souza,Ligia Cristina Kalb
author_facet Souza,Ligia Cristina Kalb
Pinho,Rosana Elisa Gonçalves Gonçalves
Lima,Carla Vanessa de Paula
Fragoso,Stênio Perdigão
Soares,Maurilio José
author_role author
author2 Pinho,Rosana Elisa Gonçalves Gonçalves
Lima,Carla Vanessa de Paula
Fragoso,Stênio Perdigão
Soares,Maurilio José
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Souza,Ligia Cristina Kalb
Pinho,Rosana Elisa Gonçalves Gonçalves
Lima,Carla Vanessa de Paula
Fragoso,Stênio Perdigão
Soares,Maurilio José
dc.subject.por.fl_str_mv actin
confocal microscopy
trypanosomatids
Trypanosoma cruzi
topic actin
confocal microscopy
trypanosomatids
Trypanosoma cruzi
dc.description.none.fl_txt_mv Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.
description Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500631
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000500631
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0074-0276108052013015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.108 n.5 2013
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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