A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays

Detalhes bibliográficos
Autor(a) principal: Cirak,Meltem Yalinay
Data de Publicação: 2005
Outros Autores: Rota,Seyyal, Maral,Isil, Turet,Sevgi, Sindel,Sukru
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300008
Resumo: The clinical value of an in-house cytomegalovirus nested polymerase chain reaction (CMV-PCR) and a commercial molecular assay hybrid capture CMV DNA assay (HCA) was evaluated in monitoring a group of renal transplant patients for six months follow up. In this study, the sensitivity, specificity, positive predictive value, and negative predictive value of nested CMV DNA PCR assay and HCA at the beginning of the study were 70, 42.9, 46.7, 66.7, and 60, 78.6, 66.7, and 73.3% respectively. After six months, they were 80, 66.7, 80, 66.7 for CMV PCR and 73.3, 88.9, 91.7, 66.7% for HCA respectively. These results indicate that in monitoring and predicting CMV infections in renal transplant recipients, not only qualitative but also quantitative assays must be used together in order to decide the preemptive strategies.
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spelling A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assayscytomegaloviruspolymerase chain reactionhybridizationThe clinical value of an in-house cytomegalovirus nested polymerase chain reaction (CMV-PCR) and a commercial molecular assay hybrid capture CMV DNA assay (HCA) was evaluated in monitoring a group of renal transplant patients for six months follow up. In this study, the sensitivity, specificity, positive predictive value, and negative predictive value of nested CMV DNA PCR assay and HCA at the beginning of the study were 70, 42.9, 46.7, 66.7, and 60, 78.6, 66.7, and 73.3% respectively. After six months, they were 80, 66.7, 80, 66.7 for CMV PCR and 73.3, 88.9, 91.7, 66.7% for HCA respectively. These results indicate that in monitoring and predicting CMV infections in renal transplant recipients, not only qualitative but also quantitative assays must be used together in order to decide the preemptive strategies.Instituto Oswaldo Cruz, Ministério da Saúde2005-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300008Memórias do Instituto Oswaldo Cruz v.100 n.3 2005reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762005000300008info:eu-repo/semantics/openAccessCirak,Meltem YalinayRota,SeyyalMaral,IsilTuret,SevgiSindel,Sukrueng2020-04-25T17:49:24Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:13:07.108Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
title A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
spellingShingle A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
Cirak,Meltem Yalinay
cytomegalovirus
polymerase chain reaction
hybridization
title_short A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
title_full A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
title_fullStr A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
title_full_unstemmed A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
title_sort A follow up study of cytomegalovirus infection in a group of Turkish renal transplant recipients using molecular assays
author Cirak,Meltem Yalinay
author_facet Cirak,Meltem Yalinay
Rota,Seyyal
Maral,Isil
Turet,Sevgi
Sindel,Sukru
author_role author
author2 Rota,Seyyal
Maral,Isil
Turet,Sevgi
Sindel,Sukru
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cirak,Meltem Yalinay
Rota,Seyyal
Maral,Isil
Turet,Sevgi
Sindel,Sukru
dc.subject.por.fl_str_mv cytomegalovirus
polymerase chain reaction
hybridization
topic cytomegalovirus
polymerase chain reaction
hybridization
dc.description.none.fl_txt_mv The clinical value of an in-house cytomegalovirus nested polymerase chain reaction (CMV-PCR) and a commercial molecular assay hybrid capture CMV DNA assay (HCA) was evaluated in monitoring a group of renal transplant patients for six months follow up. In this study, the sensitivity, specificity, positive predictive value, and negative predictive value of nested CMV DNA PCR assay and HCA at the beginning of the study were 70, 42.9, 46.7, 66.7, and 60, 78.6, 66.7, and 73.3% respectively. After six months, they were 80, 66.7, 80, 66.7 for CMV PCR and 73.3, 88.9, 91.7, 66.7% for HCA respectively. These results indicate that in monitoring and predicting CMV infections in renal transplant recipients, not only qualitative but also quantitative assays must be used together in order to decide the preemptive strategies.
description The clinical value of an in-house cytomegalovirus nested polymerase chain reaction (CMV-PCR) and a commercial molecular assay hybrid capture CMV DNA assay (HCA) was evaluated in monitoring a group of renal transplant patients for six months follow up. In this study, the sensitivity, specificity, positive predictive value, and negative predictive value of nested CMV DNA PCR assay and HCA at the beginning of the study were 70, 42.9, 46.7, 66.7, and 60, 78.6, 66.7, and 73.3% respectively. After six months, they were 80, 66.7, 80, 66.7 for CMV PCR and 73.3, 88.9, 91.7, 66.7% for HCA respectively. These results indicate that in monitoring and predicting CMV infections in renal transplant recipients, not only qualitative but also quantitative assays must be used together in order to decide the preemptive strategies.
publishDate 2005
dc.date.none.fl_str_mv 2005-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300008
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000300008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762005000300008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.100 n.3 2005
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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