SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar

Bibliographic Details
Main Author: Guimarães,Taísa Coelho
Publication Date: 2022
Other Authors: Marques,Barbara Bruno Fagundes, Tinoco,Justine Monteiro Monnerat, Porto,Luís Cristóvão Moraes Sobrino, Tinoco,Eduardo Muniz Barretto, Fischer,Ricardo Guimarães
Format: Article
Language: eng
Source: Brazilian Dental Journal
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200068
Summary: Abstract The World Health Organization has declared the widespread spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency. The standard gold test for detecting the virus is the RT-PCR, performed from nasopharyngeal swab (NPS) samples. However, this test may be uncomfortable for the patient and requires specific training and attire from the health professional responsible for collecting the sample. Therefore, the search for alternative ways to collect samples that may be used in the diagnosis of COVID-19 is relevant. This study aimed to compare the results obtained from NPS and saliva samples. NPS and saliva samples were collected from 189 symptomatic outpatients suspected of COVID-19, who came to Piquet Carneiro Polyclinic. RNA extraction was performed using the Bio-Gene DNA/RNA Viral Extraction kit (Bioclin®). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) reactions used the Molecular SARS-CoV-2 (E / RP) kit (Bio-Manguinhos). The results indicated that 142 showed a non-detectable result (ND), while 47 showed a detectable result (D). Among the 142 "ND", 137 (94.4%) saliva samples obtained the same result, while 5 samples (3.4%) were "D". Among the 47 "D" swab samples, 35 (74.4%) showed the same result in the saliva samples. The sensitivity of the saliva test was 0.74 and the specificity was 0.97. The positive predictive value was 0.88 while the negative predictive value was 0.92. The results showed that detection of Sars-CoV-2 using saliva samples showed high sensitivity and specificity compared to nasopharyngeal swabs.
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spelling SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similarsalivareal-time RT-PCRnasopharyngeal swabCovid-19SARS CoV-2Abstract The World Health Organization has declared the widespread spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency. The standard gold test for detecting the virus is the RT-PCR, performed from nasopharyngeal swab (NPS) samples. However, this test may be uncomfortable for the patient and requires specific training and attire from the health professional responsible for collecting the sample. Therefore, the search for alternative ways to collect samples that may be used in the diagnosis of COVID-19 is relevant. This study aimed to compare the results obtained from NPS and saliva samples. NPS and saliva samples were collected from 189 symptomatic outpatients suspected of COVID-19, who came to Piquet Carneiro Polyclinic. RNA extraction was performed using the Bio-Gene DNA/RNA Viral Extraction kit (Bioclin®). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) reactions used the Molecular SARS-CoV-2 (E / RP) kit (Bio-Manguinhos). The results indicated that 142 showed a non-detectable result (ND), while 47 showed a detectable result (D). Among the 142 "ND", 137 (94.4%) saliva samples obtained the same result, while 5 samples (3.4%) were "D". Among the 47 "D" swab samples, 35 (74.4%) showed the same result in the saliva samples. The sensitivity of the saliva test was 0.74 and the specificity was 0.97. The positive predictive value was 0.88 while the negative predictive value was 0.92. The results showed that detection of Sars-CoV-2 using saliva samples showed high sensitivity and specificity compared to nasopharyngeal swabs.Fundação Odontológica de Ribeirão Preto2022-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200068Brazilian Dental Journal v.33 n.2 2022reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202204591info:eu-repo/semantics/openAccessGuimarães,Taísa CoelhoMarques,Barbara Bruno FagundesTinoco,Justine Monteiro MonneratPorto,Luís Cristóvão Moraes SobrinoTinoco,Eduardo Muniz BarrettoFischer,Ricardo Guimarãeseng2022-04-26T00:00:00Zoai:scielo:S0103-64402022000200068Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2022-04-26T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false
dc.title.none.fl_str_mv SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
title SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
spellingShingle SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
Guimarães,Taísa Coelho
saliva
real-time RT-PCR
nasopharyngeal swab
Covid-19
SARS CoV-2
title_short SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
title_full SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
title_fullStr SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
title_full_unstemmed SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
title_sort SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
author Guimarães,Taísa Coelho
author_facet Guimarães,Taísa Coelho
Marques,Barbara Bruno Fagundes
Tinoco,Justine Monteiro Monnerat
Porto,Luís Cristóvão Moraes Sobrino
Tinoco,Eduardo Muniz Barretto
Fischer,Ricardo Guimarães
author_role author
author2 Marques,Barbara Bruno Fagundes
Tinoco,Justine Monteiro Monnerat
Porto,Luís Cristóvão Moraes Sobrino
Tinoco,Eduardo Muniz Barretto
Fischer,Ricardo Guimarães
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães,Taísa Coelho
Marques,Barbara Bruno Fagundes
Tinoco,Justine Monteiro Monnerat
Porto,Luís Cristóvão Moraes Sobrino
Tinoco,Eduardo Muniz Barretto
Fischer,Ricardo Guimarães
dc.subject.por.fl_str_mv saliva
real-time RT-PCR
nasopharyngeal swab
Covid-19
SARS CoV-2
topic saliva
real-time RT-PCR
nasopharyngeal swab
Covid-19
SARS CoV-2
description Abstract The World Health Organization has declared the widespread spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency. The standard gold test for detecting the virus is the RT-PCR, performed from nasopharyngeal swab (NPS) samples. However, this test may be uncomfortable for the patient and requires specific training and attire from the health professional responsible for collecting the sample. Therefore, the search for alternative ways to collect samples that may be used in the diagnosis of COVID-19 is relevant. This study aimed to compare the results obtained from NPS and saliva samples. NPS and saliva samples were collected from 189 symptomatic outpatients suspected of COVID-19, who came to Piquet Carneiro Polyclinic. RNA extraction was performed using the Bio-Gene DNA/RNA Viral Extraction kit (Bioclin®). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) reactions used the Molecular SARS-CoV-2 (E / RP) kit (Bio-Manguinhos). The results indicated that 142 showed a non-detectable result (ND), while 47 showed a detectable result (D). Among the 142 "ND", 137 (94.4%) saliva samples obtained the same result, while 5 samples (3.4%) were "D". Among the 47 "D" swab samples, 35 (74.4%) showed the same result in the saliva samples. The sensitivity of the saliva test was 0.74 and the specificity was 0.97. The positive predictive value was 0.88 while the negative predictive value was 0.92. The results showed that detection of Sars-CoV-2 using saliva samples showed high sensitivity and specificity compared to nasopharyngeal swabs.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200068
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200068
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-6440202204591
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
publisher.none.fl_str_mv Fundação Odontológica de Ribeirão Preto
dc.source.none.fl_str_mv Brazilian Dental Journal v.33 n.2 2022
reponame:Brazilian Dental Journal
instname:Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron:FUNORP
instname_str Fundação Odontológica de Ribeirão Preto (FUNORP)
instacron_str FUNORP
institution FUNORP
reponame_str Brazilian Dental Journal
collection Brazilian Dental Journal
repository.name.fl_str_mv Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)
repository.mail.fl_str_mv bdj@forp.usp.br||sergio@fosjc.unesp.br
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