SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar
Main Author: | |
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Publication Date: | 2022 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Dental Journal |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200068 |
Summary: | Abstract The World Health Organization has declared the widespread spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency. The standard gold test for detecting the virus is the RT-PCR, performed from nasopharyngeal swab (NPS) samples. However, this test may be uncomfortable for the patient and requires specific training and attire from the health professional responsible for collecting the sample. Therefore, the search for alternative ways to collect samples that may be used in the diagnosis of COVID-19 is relevant. This study aimed to compare the results obtained from NPS and saliva samples. NPS and saliva samples were collected from 189 symptomatic outpatients suspected of COVID-19, who came to Piquet Carneiro Polyclinic. RNA extraction was performed using the Bio-Gene DNA/RNA Viral Extraction kit (Bioclin®). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) reactions used the Molecular SARS-CoV-2 (E / RP) kit (Bio-Manguinhos). The results indicated that 142 showed a non-detectable result (ND), while 47 showed a detectable result (D). Among the 142 "ND", 137 (94.4%) saliva samples obtained the same result, while 5 samples (3.4%) were "D". Among the 47 "D" swab samples, 35 (74.4%) showed the same result in the saliva samples. The sensitivity of the saliva test was 0.74 and the specificity was 0.97. The positive predictive value was 0.88 while the negative predictive value was 0.92. The results showed that detection of Sars-CoV-2 using saliva samples showed high sensitivity and specificity compared to nasopharyngeal swabs. |
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SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similarsalivareal-time RT-PCRnasopharyngeal swabCovid-19SARS CoV-2Abstract The World Health Organization has declared the widespread spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency. The standard gold test for detecting the virus is the RT-PCR, performed from nasopharyngeal swab (NPS) samples. However, this test may be uncomfortable for the patient and requires specific training and attire from the health professional responsible for collecting the sample. Therefore, the search for alternative ways to collect samples that may be used in the diagnosis of COVID-19 is relevant. This study aimed to compare the results obtained from NPS and saliva samples. NPS and saliva samples were collected from 189 symptomatic outpatients suspected of COVID-19, who came to Piquet Carneiro Polyclinic. RNA extraction was performed using the Bio-Gene DNA/RNA Viral Extraction kit (Bioclin®). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) reactions used the Molecular SARS-CoV-2 (E / RP) kit (Bio-Manguinhos). The results indicated that 142 showed a non-detectable result (ND), while 47 showed a detectable result (D). Among the 142 "ND", 137 (94.4%) saliva samples obtained the same result, while 5 samples (3.4%) were "D". Among the 47 "D" swab samples, 35 (74.4%) showed the same result in the saliva samples. The sensitivity of the saliva test was 0.74 and the specificity was 0.97. The positive predictive value was 0.88 while the negative predictive value was 0.92. The results showed that detection of Sars-CoV-2 using saliva samples showed high sensitivity and specificity compared to nasopharyngeal swabs.Fundação Odontológica de Ribeirão Preto2022-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200068Brazilian Dental Journal v.33 n.2 2022reponame:Brazilian Dental Journalinstname:Fundação Odontológica de Ribeirão Preto (FUNORP)instacron:FUNORP10.1590/0103-6440202204591info:eu-repo/semantics/openAccessGuimarães,Taísa CoelhoMarques,Barbara Bruno FagundesTinoco,Justine Monteiro MonneratPorto,Luís Cristóvão Moraes SobrinoTinoco,Eduardo Muniz BarrettoFischer,Ricardo Guimarãeseng2022-04-26T00:00:00Zoai:scielo:S0103-64402022000200068Revistahttps://www.scielo.br/j/bdj/https://old.scielo.br/oai/scielo-oai.phpbdj@forp.usp.br||sergio@fosjc.unesp.br1806-47600103-6440opendoar:2022-04-26T00:00Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP)false |
dc.title.none.fl_str_mv |
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar |
title |
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar |
spellingShingle |
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar Guimarães,Taísa Coelho saliva real-time RT-PCR nasopharyngeal swab Covid-19 SARS CoV-2 |
title_short |
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar |
title_full |
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar |
title_fullStr |
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar |
title_full_unstemmed |
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar |
title_sort |
SARS-COV-2 detection in saliva and nasopharyngeal swabs using RT-PCR was similar |
author |
Guimarães,Taísa Coelho |
author_facet |
Guimarães,Taísa Coelho Marques,Barbara Bruno Fagundes Tinoco,Justine Monteiro Monnerat Porto,Luís Cristóvão Moraes Sobrino Tinoco,Eduardo Muniz Barretto Fischer,Ricardo Guimarães |
author_role |
author |
author2 |
Marques,Barbara Bruno Fagundes Tinoco,Justine Monteiro Monnerat Porto,Luís Cristóvão Moraes Sobrino Tinoco,Eduardo Muniz Barretto Fischer,Ricardo Guimarães |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Guimarães,Taísa Coelho Marques,Barbara Bruno Fagundes Tinoco,Justine Monteiro Monnerat Porto,Luís Cristóvão Moraes Sobrino Tinoco,Eduardo Muniz Barretto Fischer,Ricardo Guimarães |
dc.subject.por.fl_str_mv |
saliva real-time RT-PCR nasopharyngeal swab Covid-19 SARS CoV-2 |
topic |
saliva real-time RT-PCR nasopharyngeal swab Covid-19 SARS CoV-2 |
description |
Abstract The World Health Organization has declared the widespread spread of SARS-CoV-2 and its associated disease (COVID-19) a public health emergency. The standard gold test for detecting the virus is the RT-PCR, performed from nasopharyngeal swab (NPS) samples. However, this test may be uncomfortable for the patient and requires specific training and attire from the health professional responsible for collecting the sample. Therefore, the search for alternative ways to collect samples that may be used in the diagnosis of COVID-19 is relevant. This study aimed to compare the results obtained from NPS and saliva samples. NPS and saliva samples were collected from 189 symptomatic outpatients suspected of COVID-19, who came to Piquet Carneiro Polyclinic. RNA extraction was performed using the Bio-Gene DNA/RNA Viral Extraction kit (Bioclin®). Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) reactions used the Molecular SARS-CoV-2 (E / RP) kit (Bio-Manguinhos). The results indicated that 142 showed a non-detectable result (ND), while 47 showed a detectable result (D). Among the 142 "ND", 137 (94.4%) saliva samples obtained the same result, while 5 samples (3.4%) were "D". Among the 47 "D" swab samples, 35 (74.4%) showed the same result in the saliva samples. The sensitivity of the saliva test was 0.74 and the specificity was 0.97. The positive predictive value was 0.88 while the negative predictive value was 0.92. The results showed that detection of Sars-CoV-2 using saliva samples showed high sensitivity and specificity compared to nasopharyngeal swabs. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200068 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402022000200068 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-6440202204591 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
publisher.none.fl_str_mv |
Fundação Odontológica de Ribeirão Preto |
dc.source.none.fl_str_mv |
Brazilian Dental Journal v.33 n.2 2022 reponame:Brazilian Dental Journal instname:Fundação Odontológica de Ribeirão Preto (FUNORP) instacron:FUNORP |
instname_str |
Fundação Odontológica de Ribeirão Preto (FUNORP) |
instacron_str |
FUNORP |
institution |
FUNORP |
reponame_str |
Brazilian Dental Journal |
collection |
Brazilian Dental Journal |
repository.name.fl_str_mv |
Brazilian Dental Journal - Fundação Odontológica de Ribeirão Preto (FUNORP) |
repository.mail.fl_str_mv |
bdj@forp.usp.br||sergio@fosjc.unesp.br |
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1754204096625115136 |