Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/3944 |
Resumo: | In a single-stage process with the simultaneous addition of β -galactosidase and lactic culture, the lactose conversion, the processing time, viscosity and syneresis were evaluated. Fermentation was promoted by lactic culture containing two probiotic microorganisms, Bifidobacterium animalis and Lactobacillus acidophilus, associated with the typical microorganisms of yogurt. An enzymatic preparation containing β -galactosidases from Kluyveromyces lactis and Aspergillus niger was used. A central composite design (23 trials plus three central points) was proposed in order to evaluate the effects of initial lactose concentration, enzyme concentration and the time of addition of the enzyme. The following conditions were established: initial lactose concentration of 91 g L-1, enzyme concentration of 0.5 g L-1 and enzyme addition at the beginning of fermentation. In these conditions, a decrease in processing time (from 4.55 to 3.68 h) and an increase in lactose conversion (from 15.2 to 97.9%) were observed in relation to the fermentation without enzyme addition, and no detrimental changes in some physical properties of yogurt (viscosity, and syneresis). |
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Martins, André RosaMonteiro, Ricardo LemosBurkert, Janaina Fernandes de MedeirosBurkert, Carlos André Veiga2013-09-27T20:21:33Z2013-09-27T20:21:33Z2012Martins, André Rosa "et al". Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content. Ciência e Agrotecnologia (UFLA), v. 36, p. 551-559, 2012. Disponível em:( http://www.scielo.br/pdf/cagro/v36n5/v36n5a08.pdf )Acesso em: 24 set. 2013.http://repositorio.furg.br/handle/1/3944In a single-stage process with the simultaneous addition of β -galactosidase and lactic culture, the lactose conversion, the processing time, viscosity and syneresis were evaluated. Fermentation was promoted by lactic culture containing two probiotic microorganisms, Bifidobacterium animalis and Lactobacillus acidophilus, associated with the typical microorganisms of yogurt. An enzymatic preparation containing β -galactosidases from Kluyveromyces lactis and Aspergillus niger was used. A central composite design (23 trials plus three central points) was proposed in order to evaluate the effects of initial lactose concentration, enzyme concentration and the time of addition of the enzyme. The following conditions were established: initial lactose concentration of 91 g L-1, enzyme concentration of 0.5 g L-1 and enzyme addition at the beginning of fermentation. In these conditions, a decrease in processing time (from 4.55 to 3.68 h) and an increase in lactose conversion (from 15.2 to 97.9%) were observed in relation to the fermentation without enzyme addition, and no detrimental changes in some physical properties of yogurt (viscosity, and syneresis).No processo em um único estágio com a adição simultânea de β -galactosidase e de cultura lática, foram avaliadas a conversão da lactose, o tempo de processamento, a viscosidade e a sinérese. A fermentação foi promovida por cultura láctica, contendo dois micro-organismos probióticos, Bifidobacterium animalis e Lactobacillus acidophilus, associados aos micro-organismos característicos do iogurte. Foi utilizado um preparado enzimático contendo β -galactosidases obtidas de Kluyveromyces lactis e de Aspergillus niger. Os efeitos da concentração inicial de lactose,concentração de enzima e tempo de adição da enzima foram avaliados por meio de um planejamento experimental (23 ensaios mais três pontos centrais). As seguintes condições foram estabelecidas: concentração inicial de lactose de 91 g L-1, concentração de enzima de 0.5 g L-1 e adição da enzima no início da fermentação. Nessas condições, um decréscimo no tempo de processamento (de 4,55 h para 3,68 h) e um incremento na conversão de lactose (de 15,2% para 97,9%) foram observados em relação à fermentação sem a adição da enzima, sem alterações prejudiciais em algumas propriedades físicas do iogurte (viscosidade, e sinérese).engEnzymatic catalysisLactose hydrolysisYogurt physical propertiesβ -galactosidaseSimultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose contentHidrólise enzimática e fermentação lática simultâneas na obtenção de um iogurte com baixo teor de lactoseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALSimultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content.pdfSimultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content.pdfapplication/pdf476579https://repositorio.furg.br/bitstream/1/3944/1/Simultaneous%20enzymatic%20hydrolysis%20and%20lactic%20fermentation%20to%20obtain%20a%20yogurt%20with%20low%20lactose%20content.pdfee3e353e58d9094cee7f15ca7e0229b8MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81678https://repositorio.furg.br/bitstream/1/3944/2/license.txtbd159dc78a9e85799a1e2ce197b491ceMD52open access1/39442014-08-29 14:59:05.358open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-08-29T17:59:05Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content |
dc.title.alternative.pt_BR.fl_str_mv |
Hidrólise enzimática e fermentação lática simultâneas na obtenção de um iogurte com baixo teor de lactose |
title |
Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content |
spellingShingle |
Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content Martins, André Rosa Enzymatic catalysis Lactose hydrolysis Yogurt physical properties β -galactosidase |
title_short |
Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content |
title_full |
Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content |
title_fullStr |
Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content |
title_full_unstemmed |
Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content |
title_sort |
Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content |
author |
Martins, André Rosa |
author_facet |
Martins, André Rosa Monteiro, Ricardo Lemos Burkert, Janaina Fernandes de Medeiros Burkert, Carlos André Veiga |
author_role |
author |
author2 |
Monteiro, Ricardo Lemos Burkert, Janaina Fernandes de Medeiros Burkert, Carlos André Veiga |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Martins, André Rosa Monteiro, Ricardo Lemos Burkert, Janaina Fernandes de Medeiros Burkert, Carlos André Veiga |
dc.subject.por.fl_str_mv |
Enzymatic catalysis Lactose hydrolysis Yogurt physical properties β -galactosidase |
topic |
Enzymatic catalysis Lactose hydrolysis Yogurt physical properties β -galactosidase |
description |
In a single-stage process with the simultaneous addition of β -galactosidase and lactic culture, the lactose conversion, the processing time, viscosity and syneresis were evaluated. Fermentation was promoted by lactic culture containing two probiotic microorganisms, Bifidobacterium animalis and Lactobacillus acidophilus, associated with the typical microorganisms of yogurt. An enzymatic preparation containing β -galactosidases from Kluyveromyces lactis and Aspergillus niger was used. A central composite design (23 trials plus three central points) was proposed in order to evaluate the effects of initial lactose concentration, enzyme concentration and the time of addition of the enzyme. The following conditions were established: initial lactose concentration of 91 g L-1, enzyme concentration of 0.5 g L-1 and enzyme addition at the beginning of fermentation. In these conditions, a decrease in processing time (from 4.55 to 3.68 h) and an increase in lactose conversion (from 15.2 to 97.9%) were observed in relation to the fermentation without enzyme addition, and no detrimental changes in some physical properties of yogurt (viscosity, and syneresis). |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2013-09-27T20:21:33Z |
dc.date.available.fl_str_mv |
2013-09-27T20:21:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Martins, André Rosa "et al". Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content. Ciência e Agrotecnologia (UFLA), v. 36, p. 551-559, 2012. Disponível em:( http://www.scielo.br/pdf/cagro/v36n5/v36n5a08.pdf )Acesso em: 24 set. 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/3944 |
identifier_str_mv |
Martins, André Rosa "et al". Simultaneous enzymatic hydrolysis and lactic fermentation to obtain a yogurt with low lactose content. Ciência e Agrotecnologia (UFLA), v. 36, p. 551-559, 2012. Disponível em:( http://www.scielo.br/pdf/cagro/v36n5/v36n5a08.pdf )Acesso em: 24 set. 2013. |
url |
http://repositorio.furg.br/handle/1/3944 |
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eng |
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eng |
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