The laboratory as a tool to qualify tuberculosis diagnostic

Detalhes bibliográficos
Autor(a) principal: Honscha, Gunther
Data de Publicação: 2008
Outros Autores: Groll, Andrea Von, Valença, Mariana Soares, Soares, Daniela Fernandes Ramos, Sanchotene, Karine Ortiz, Scaini, Carlos James, Ribeiro, Marta Osório, Silva, Pedro Eduardo Almeida da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/3468
Resumo: OBJECTIVES: To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST). METHODS: A total of 554 samples from 269 patients were tested for smear microscopy using Kinyoun stain. Culture was performed in Ogawa-Kudoh medium and species identification was performed using the IS6110 amplified region. DST for rifampicin, isoniazid (INH) and streptomycin were carried out using the Resazurin assay. RESULTS: Cultures augmented the number of cases diagnosed by 22.1%, IS6110 amplification identified all Mycobacterium tuberculosis strains thus isolated and DST detected three strains resistant to INH and one multidrug-resistant strain. CONCLUSION: Simultaneous use of different techniques enhanced culture yield, species identification and detection of drug resistance even in a laboratory with limited facilities.
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spelling Honscha, GuntherGroll, Andrea VonValença, Mariana SoaresSoares, Daniela Fernandes RamosSanchotene, Karine OrtizScaini, Carlos JamesRibeiro, Marta OsórioSilva, Pedro Eduardo Almeida da2013-06-06T00:49:22Z2013-06-06T00:49:22Z2008HONSCHA, Gunther et al. The laboratory as a tool to qualify tuberculosis diagnostic. The International Journal of Tuberculosis and Lung Disease, v. 12, n. 2, p. 218-220, 2008. Disponível em: <http://tbevidence.org/documents/rescentre/sop/studies/Ogawa-Kudoh%20method%20for%20TB/3.pdf>. Acesso em: 01 set. 2012.http://repositorio.furg.br/handle/1/3468OBJECTIVES: To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST). METHODS: A total of 554 samples from 269 patients were tested for smear microscopy using Kinyoun stain. Culture was performed in Ogawa-Kudoh medium and species identification was performed using the IS6110 amplified region. DST for rifampicin, isoniazid (INH) and streptomycin were carried out using the Resazurin assay. RESULTS: Cultures augmented the number of cases diagnosed by 22.1%, IS6110 amplification identified all Mycobacterium tuberculosis strains thus isolated and DST detected three strains resistant to INH and one multidrug-resistant strain. CONCLUSION: Simultaneous use of different techniques enhanced culture yield, species identification and detection of drug resistance even in a laboratory with limited facilities.OBJECTIFS : Pour évaluer les performances du diagnostic de laboratoire de la tuberculose, des échantillons cliniques ont été soumis à la culture, à l’identification de l’espèce et aux tests de sensibilité (DST). MÉTHODES : 554 échantillons provenant de 269 patients ont été testés par examen microscopique des frottis après coloration de Kinyoun. Les cultures ont été faites sur milieu d’Ogawa-Kudoh et l’identification des espèces a utilisé la région amplifiée IS6110. Les tests de sensibilité à la rifampicine, l’isoniazide (INH) et la streptomycine ont été menés par la technique de Resazurine. RÉSULTATS : Les cultures ont augmenté le nombre de cas diagnostiqués de 22,1%, alors que l’amplification d’IS6110 a identifié chaque souche isolée de Mycobacterium tuberculosis et que les DST ont détecté trois souches résistantes à INH et une souche multirésistante. CONCLUSION : L’utilisation simultanée de différentes techniques a augmenté le rendement des cultures, l’identification des espèces et la détection de la résistance aux médicaments, même dans un laboratoire ayant des ressources limitées.OBJETIVOS : Con el propósito de evaluar la eficacia del diagnóstico de laboratorio de la tuberculosis, se sometió una serie de muestras clínicas a cultivo, identificación de especie y pruebas de sensibilidad a los medicamentos. MÉTODOS : Se analizaron 554 muestras provenientes de 269 pacientes mediante examen microscópico del esputo con coloración de Kinyoun, cultivos en medio OgawaKudoh e identificación de especies por amplificación de la región IS6110. Las pruebas de sensibilidad a rifampicina, isoniazida (INH) y estreptomicina se realizaron con el ensayo de azul de Alamar (Resazurin). RESULTADOS : Los cultivos aumentaron un 22,1% el número de casos diagnosticados y con la amplificación de la región IS6110 se identificaron todas las cepas de Mycobacterium tuberculosis así aisladas ; mediante las pruebas de sensibilidad se detectaron tres cepas resistentes a INH y una cepa multidrogorresistente. CONCLUSIÓN : La aplicación simultánea de diferentes técnicas aumentó la eficacia de los cultivos, la identificación de especies y las pruebas de farmacorresistencia, aún en un laboratorio con medios limitados.engTuberculosisCultureMycobacterium tuberculosisDiagnosisThe laboratory as a tool to qualify tuberculosis diagnosticinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALThe laboratory as a tool to qualify tuberculosis diagnosis.pdfThe laboratory as a tool to qualify tuberculosis diagnosis.pdfapplication/pdf85079https://repositorio.furg.br/bitstream/1/3468/1/The%20laboratory%20as%20a%20tool%20to%20qualify%20tuberculosis%20diagnosis.pdfd26e955df126dc0b81dbc6340167dac9MD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81678https://repositorio.furg.br/bitstream/1/3468/2/license.txtd3be63d3b3eee02729709361dac69efeMD52open access1/34682019-12-03 17:29:26.243open accessoai:repositorio.furg.br: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ório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2019-12-03T20:29:26Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.pt_BR.fl_str_mv The laboratory as a tool to qualify tuberculosis diagnostic
title The laboratory as a tool to qualify tuberculosis diagnostic
spellingShingle The laboratory as a tool to qualify tuberculosis diagnostic
Honscha, Gunther
Tuberculosis
Culture
Mycobacterium tuberculosis
Diagnosis
title_short The laboratory as a tool to qualify tuberculosis diagnostic
title_full The laboratory as a tool to qualify tuberculosis diagnostic
title_fullStr The laboratory as a tool to qualify tuberculosis diagnostic
title_full_unstemmed The laboratory as a tool to qualify tuberculosis diagnostic
title_sort The laboratory as a tool to qualify tuberculosis diagnostic
author Honscha, Gunther
author_facet Honscha, Gunther
Groll, Andrea Von
Valença, Mariana Soares
Soares, Daniela Fernandes Ramos
Sanchotene, Karine Ortiz
Scaini, Carlos James
Ribeiro, Marta Osório
Silva, Pedro Eduardo Almeida da
author_role author
author2 Groll, Andrea Von
Valença, Mariana Soares
Soares, Daniela Fernandes Ramos
Sanchotene, Karine Ortiz
Scaini, Carlos James
Ribeiro, Marta Osório
Silva, Pedro Eduardo Almeida da
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Honscha, Gunther
Groll, Andrea Von
Valença, Mariana Soares
Soares, Daniela Fernandes Ramos
Sanchotene, Karine Ortiz
Scaini, Carlos James
Ribeiro, Marta Osório
Silva, Pedro Eduardo Almeida da
dc.subject.por.fl_str_mv Tuberculosis
Culture
Mycobacterium tuberculosis
Diagnosis
topic Tuberculosis
Culture
Mycobacterium tuberculosis
Diagnosis
description OBJECTIVES: To evaluate the performance of laboratory diagnosis of tuberculosis, clinical samples underwent culture, species identification and drug susceptibility testing (DST). METHODS: A total of 554 samples from 269 patients were tested for smear microscopy using Kinyoun stain. Culture was performed in Ogawa-Kudoh medium and species identification was performed using the IS6110 amplified region. DST for rifampicin, isoniazid (INH) and streptomycin were carried out using the Resazurin assay. RESULTS: Cultures augmented the number of cases diagnosed by 22.1%, IS6110 amplification identified all Mycobacterium tuberculosis strains thus isolated and DST detected three strains resistant to INH and one multidrug-resistant strain. CONCLUSION: Simultaneous use of different techniques enhanced culture yield, species identification and detection of drug resistance even in a laboratory with limited facilities.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2013-06-06T00:49:22Z
dc.date.available.fl_str_mv 2013-06-06T00:49:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HONSCHA, Gunther et al. The laboratory as a tool to qualify tuberculosis diagnostic. The International Journal of Tuberculosis and Lung Disease, v. 12, n. 2, p. 218-220, 2008. Disponível em: <http://tbevidence.org/documents/rescentre/sop/studies/Ogawa-Kudoh%20method%20for%20TB/3.pdf>. Acesso em: 01 set. 2012.
dc.identifier.uri.fl_str_mv http://repositorio.furg.br/handle/1/3468
identifier_str_mv HONSCHA, Gunther et al. The laboratory as a tool to qualify tuberculosis diagnostic. The International Journal of Tuberculosis and Lung Disease, v. 12, n. 2, p. 218-220, 2008. Disponível em: <http://tbevidence.org/documents/rescentre/sop/studies/Ogawa-Kudoh%20method%20for%20TB/3.pdf>. Acesso em: 01 set. 2012.
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