Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis

• Autor(a) principal: Santos, Elisane Odriosolla dos
• Data de Publicação: 2012
• Outros Autores: Michelon, Mariano, Furlong, Eliana Badiale, Burkert, Janaina Fernandes de Medeiros, Kalil, Susana Juliano, Burkert, Carlos André Veiga
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da FURG (RI FURG)
• Texto Completo: http://repositorio.furg.br/handle/1/4506
• topic: Raw Glycerol; Single Cell Protein; Yeast Biomass; Yarrowia Lipolytica

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).