Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4421 |
Resumo: | The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain In this study, Spirulina LEB-18 phenolic extract was assessed for its antifungal activity on 12 toxigenic strains of Fusarium graminearum isolated from barley and wheat. The susceptible metabolic pathways were assessed through the determination of structural compounds (glucosamine and ergosterol) and enzyme activity of the microorganisms’ primary metabolism. The results indicate that phenolic extracts reduced the growth rate of the toxigenic species investigated. The IC50 was obtained by applying 3 to 8% (p/p) of phenolic compounds in relation to the culture medium. The use of this natural fungicide proved promising for the inhibition of fungal multiplication, especially in terms of the inactivation of enzymatic systems (amylase and protease) of Fusarium graminearum. |
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Pagnussatt, Fernanda ArnholdKupski, LarineDarley, Francine TorallesFiloda, Paula FreitasDel Ponte, Emerson MedeirosBuffon, Jaqueline GardaFurlong, Eliana Badiale2014-07-22T19:14:31Z2014-07-22T19:14:31Z2013PAGNUSSATT, Fernanda Arnhold. et al. Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp. Food Science And Technology, v. 33, p. 75-80, 2013. Disponível em: <http://www.scielo.br/pdf/cta/v33s1/v33s1a12.pdf>. Acesso em: 27 fev. 2014.0101-2061http://repositorio.furg.br/handle/1/4421The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain In this study, Spirulina LEB-18 phenolic extract was assessed for its antifungal activity on 12 toxigenic strains of Fusarium graminearum isolated from barley and wheat. The susceptible metabolic pathways were assessed through the determination of structural compounds (glucosamine and ergosterol) and enzyme activity of the microorganisms’ primary metabolism. The results indicate that phenolic extracts reduced the growth rate of the toxigenic species investigated. The IC50 was obtained by applying 3 to 8% (p/p) of phenolic compounds in relation to the culture medium. The use of this natural fungicide proved promising for the inhibition of fungal multiplication, especially in terms of the inactivation of enzymatic systems (amylase and protease) of Fusarium graminearum.A aplicação de substâncias naturais com efeito antifúngico é motivada pela necessidade de alternativas aos métodos existentes que nem sempre são aplicáveis, eficientes ou sem risco de danos ao consumidor ou meio ambiente. Além disso, estudos para elucidar o comportamento de espécies toxigênicas mediante fungicidas naturais tornam-se necessárias, contribuindo dessa forma com a segurança alimentar. Neste trabalho, extrato fenólico de Spirulina foi utilizado para avaliar a atividade antifúngica sobre 12 cepas toxigênicas de Fusarium graminearum, isoladas de cevada e trigo. As rotas metabólicas que poderiam ser afetadas foram avaliadas através da determinação de compostos estruturais (glicosamina e ergosterol) e da atividade de enzimas do metabolismo primário dos micro-organismos. Os resultados indicaram que os extratos fenólicos reduziram a velocidade de crescimento das espécies toxigênicas estudadas. O IC50 foi obtido na concentração de 3% a 8% (p/p) de extrato fenólico. A utilização desse fungicida natural foi promissora para a inibição da multiplicação fúngica, em especial pela inativação dos sistemas enzimáticos (amilase e protease) de Fusarium graminearum.engnatural antifungal agentstructural compoundsenzymesantifúngico naturalcompostos estruturaisenzimasFusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina spMecanismo de inibição de Fusarium graminearum por compostos fenólicos extraídos de Spirulina sp.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALFusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp.pdfFusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp.pdfapplication/pdf511359https://repositorio.furg.br/bitstream/1/4421/1/Fusarium%20graminearum%20growth%20inhibition%20mechanism%20using%20phenolic%20compounds%20from%20Spirulina%20sp.pdffaeb7028c9dd2d96c1420448acaa0e0dMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.furg.br/bitstream/1/4421/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52open access1/44212014-07-22 16:14:31.868open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-07-22T19:14:31Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp |
dc.title.alternative.pt_BR.fl_str_mv |
Mecanismo de inibição de Fusarium graminearum por compostos fenólicos extraídos de Spirulina sp. |
title |
Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp |
spellingShingle |
Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp Pagnussatt, Fernanda Arnhold natural antifungal agent structural compounds enzymes antifúngico natural compostos estruturais enzimas |
title_short |
Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp |
title_full |
Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp |
title_fullStr |
Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp |
title_full_unstemmed |
Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp |
title_sort |
Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp |
author |
Pagnussatt, Fernanda Arnhold |
author_facet |
Pagnussatt, Fernanda Arnhold Kupski, Larine Darley, Francine Toralles Filoda, Paula Freitas Del Ponte, Emerson Medeiros Buffon, Jaqueline Garda Furlong, Eliana Badiale |
author_role |
author |
author2 |
Kupski, Larine Darley, Francine Toralles Filoda, Paula Freitas Del Ponte, Emerson Medeiros Buffon, Jaqueline Garda Furlong, Eliana Badiale |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pagnussatt, Fernanda Arnhold Kupski, Larine Darley, Francine Toralles Filoda, Paula Freitas Del Ponte, Emerson Medeiros Buffon, Jaqueline Garda Furlong, Eliana Badiale |
dc.subject.por.fl_str_mv |
natural antifungal agent structural compounds enzymes antifúngico natural compostos estruturais enzimas |
topic |
natural antifungal agent structural compounds enzymes antifúngico natural compostos estruturais enzimas |
description |
The application of natural antifungal substances is motivated by the need for alternatives to existing methods that are not always applicable, efficient, or that do not pose risk to consumers or the environment. Furthermore, studies on the behaviour of toxigenic species in the presence of natural fungicides have enabled their safe application in the food chain In this study, Spirulina LEB-18 phenolic extract was assessed for its antifungal activity on 12 toxigenic strains of Fusarium graminearum isolated from barley and wheat. The susceptible metabolic pathways were assessed through the determination of structural compounds (glucosamine and ergosterol) and enzyme activity of the microorganisms’ primary metabolism. The results indicate that phenolic extracts reduced the growth rate of the toxigenic species investigated. The IC50 was obtained by applying 3 to 8% (p/p) of phenolic compounds in relation to the culture medium. The use of this natural fungicide proved promising for the inhibition of fungal multiplication, especially in terms of the inactivation of enzymatic systems (amylase and protease) of Fusarium graminearum. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2014-07-22T19:14:31Z |
dc.date.available.fl_str_mv |
2014-07-22T19:14:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PAGNUSSATT, Fernanda Arnhold. et al. Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp. Food Science And Technology, v. 33, p. 75-80, 2013. Disponível em: <http://www.scielo.br/pdf/cta/v33s1/v33s1a12.pdf>. Acesso em: 27 fev. 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/4421 |
dc.identifier.issn.none.fl_str_mv |
0101-2061 |
identifier_str_mv |
PAGNUSSATT, Fernanda Arnhold. et al. Fusarium graminearum growth inhibition mechanism using phenolic compounds from Spirulina sp. Food Science And Technology, v. 33, p. 75-80, 2013. Disponível em: <http://www.scielo.br/pdf/cta/v33s1/v33s1a12.pdf>. Acesso em: 27 fev. 2014. 0101-2061 |
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http://repositorio.furg.br/handle/1/4421 |
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FURG |
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Repositório Institucional da FURG (RI FURG) |
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