Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/4149 |
Resumo: | Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solvent-free system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M. |
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Aragão, Vitor CardosoAnschau, AndréiaPorciuncula, Barbara Daniele AlmeidaThiesen, CleidiKalil, Susana JulianoBurkert, Carlos André VeigaBurkert, Janaina Fernandes de Medeiros2013-11-12T19:55:24Z2013-11-12T19:55:24Z2009ARAGÃO, Vitor Cardoso "et al". Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais. Química Nova, v. 32, n. 9, p. 2268-2272, 2009. Disponivel em: < http://www.scielo.br/scielo.php?pid=S0100-40422009000900005&script=sci_arttext > Acesso em: 09 nov. 2013.http://repositorio.furg.br/handle/1/4149Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solvent-free system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M.porEnzymatic esterificationOrganic mediaLipaseSíntese enzimática de butirato de isoamila empregando lipases microbianas comerciaisEnzymatic synthesis isoamyl butyrate employing commercial microbial lipasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALSÍNTESE ENZIMÁTICA DE BUTIRATO DE ISOAMILA EMPREGANDO LIPASES MICROBIANAS COMERCIAIS.pdfSÍNTESE ENZIMÁTICA DE BUTIRATO DE ISOAMILA EMPREGANDO LIPASES MICROBIANAS COMERCIAIS.pdfapplication/pdf471248https://repositorio.furg.br/bitstream/1/4149/1/S%c3%8dNTESE%20ENZIM%c3%81TICA%20DE%20BUTIRATO%20DE%20ISOAMILA%20EMPREGANDO%20LIPASES%20MICROBIANAS%20COMERCIAIS.pdfc4d8dd75313a5873b2621c4e6ac152dfMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.furg.br/bitstream/1/4149/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52open access1/41492014-08-29 15:00:34.68open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-08-29T18:00:34Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais |
dc.title.alternative.pt_BR.fl_str_mv |
Enzymatic synthesis isoamyl butyrate employing commercial microbial lipases |
title |
Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais |
spellingShingle |
Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais Aragão, Vitor Cardoso Enzymatic esterification Organic media Lipase |
title_short |
Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais |
title_full |
Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais |
title_fullStr |
Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais |
title_full_unstemmed |
Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais |
title_sort |
Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais |
author |
Aragão, Vitor Cardoso |
author_facet |
Aragão, Vitor Cardoso Anschau, Andréia Porciuncula, Barbara Daniele Almeida Thiesen, Cleidi Kalil, Susana Juliano Burkert, Carlos André Veiga Burkert, Janaina Fernandes de Medeiros |
author_role |
author |
author2 |
Anschau, Andréia Porciuncula, Barbara Daniele Almeida Thiesen, Cleidi Kalil, Susana Juliano Burkert, Carlos André Veiga Burkert, Janaina Fernandes de Medeiros |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Aragão, Vitor Cardoso Anschau, Andréia Porciuncula, Barbara Daniele Almeida Thiesen, Cleidi Kalil, Susana Juliano Burkert, Carlos André Veiga Burkert, Janaina Fernandes de Medeiros |
dc.subject.por.fl_str_mv |
Enzymatic esterification Organic media Lipase |
topic |
Enzymatic esterification Organic media Lipase |
description |
Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solvent-free system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2013-11-12T19:55:24Z |
dc.date.available.fl_str_mv |
2013-11-12T19:55:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAGÃO, Vitor Cardoso "et al". Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais. Química Nova, v. 32, n. 9, p. 2268-2272, 2009. Disponivel em: < http://www.scielo.br/scielo.php?pid=S0100-40422009000900005&script=sci_arttext > Acesso em: 09 nov. 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/4149 |
identifier_str_mv |
ARAGÃO, Vitor Cardoso "et al". Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais. Química Nova, v. 32, n. 9, p. 2268-2272, 2009. Disponivel em: < http://www.scielo.br/scielo.php?pid=S0100-40422009000900005&script=sci_arttext > Acesso em: 09 nov. 2013. |
url |
http://repositorio.furg.br/handle/1/4149 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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FURG |
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