Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais

Detalhes bibliográficos
Autor(a) principal: Aragão, Vitor Cardoso
Data de Publicação: 2009
Outros Autores: Anschau, Andréia, Porciuncula, Barbara Daniele Almeida, Thiesen, Cleidi, Kalil, Susana Juliano, Burkert, Carlos André Veiga, Burkert, Janaina Fernandes de Medeiros
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FURG (RI FURG)
Texto Completo: http://repositorio.furg.br/handle/1/4149
Resumo: Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solvent-free system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M.
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spelling Aragão, Vitor CardosoAnschau, AndréiaPorciuncula, Barbara Daniele AlmeidaThiesen, CleidiKalil, Susana JulianoBurkert, Carlos André VeigaBurkert, Janaina Fernandes de Medeiros2013-11-12T19:55:24Z2013-11-12T19:55:24Z2009ARAGÃO, Vitor Cardoso "et al". Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais. Química Nova, v. 32, n. 9, p. 2268-2272, 2009. Disponivel em: < http://www.scielo.br/scielo.php?pid=S0100-40422009000900005&script=sci_arttext > Acesso em: 09 nov. 2013.http://repositorio.furg.br/handle/1/4149Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solvent-free system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M.porEnzymatic esterificationOrganic mediaLipaseSíntese enzimática de butirato de isoamila empregando lipases microbianas comerciaisEnzymatic synthesis isoamyl butyrate employing commercial microbial lipasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALSÍNTESE ENZIMÁTICA DE BUTIRATO DE ISOAMILA EMPREGANDO LIPASES MICROBIANAS COMERCIAIS.pdfSÍNTESE ENZIMÁTICA DE BUTIRATO DE ISOAMILA EMPREGANDO LIPASES MICROBIANAS COMERCIAIS.pdfapplication/pdf471248https://repositorio.furg.br/bitstream/1/4149/1/S%c3%8dNTESE%20ENZIM%c3%81TICA%20DE%20BUTIRATO%20DE%20ISOAMILA%20EMPREGANDO%20LIPASES%20MICROBIANAS%20COMERCIAIS.pdfc4d8dd75313a5873b2621c4e6ac152dfMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.furg.br/bitstream/1/4149/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52open access1/41492014-08-29 15:00:34.68open accessoai:repositorio.furg.br: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Repositório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2014-08-29T18:00:34Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false
dc.title.pt_BR.fl_str_mv Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
dc.title.alternative.pt_BR.fl_str_mv Enzymatic synthesis isoamyl butyrate employing commercial microbial lipases
title Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
spellingShingle Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
Aragão, Vitor Cardoso
Enzymatic esterification
Organic media
Lipase
title_short Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
title_full Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
title_fullStr Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
title_full_unstemmed Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
title_sort Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais
author Aragão, Vitor Cardoso
author_facet Aragão, Vitor Cardoso
Anschau, Andréia
Porciuncula, Barbara Daniele Almeida
Thiesen, Cleidi
Kalil, Susana Juliano
Burkert, Carlos André Veiga
Burkert, Janaina Fernandes de Medeiros
author_role author
author2 Anschau, Andréia
Porciuncula, Barbara Daniele Almeida
Thiesen, Cleidi
Kalil, Susana Juliano
Burkert, Carlos André Veiga
Burkert, Janaina Fernandes de Medeiros
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Aragão, Vitor Cardoso
Anschau, Andréia
Porciuncula, Barbara Daniele Almeida
Thiesen, Cleidi
Kalil, Susana Juliano
Burkert, Carlos André Veiga
Burkert, Janaina Fernandes de Medeiros
dc.subject.por.fl_str_mv Enzymatic esterification
Organic media
Lipase
topic Enzymatic esterification
Organic media
Lipase
description Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solvent-free system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2013-11-12T19:55:24Z
dc.date.available.fl_str_mv 2013-11-12T19:55:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv ARAGÃO, Vitor Cardoso "et al". Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais. Química Nova, v. 32, n. 9, p. 2268-2272, 2009. Disponivel em: < http://www.scielo.br/scielo.php?pid=S0100-40422009000900005&script=sci_arttext > Acesso em: 09 nov. 2013.
dc.identifier.uri.fl_str_mv http://repositorio.furg.br/handle/1/4149
identifier_str_mv ARAGÃO, Vitor Cardoso "et al". Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais. Química Nova, v. 32, n. 9, p. 2268-2272, 2009. Disponivel em: < http://www.scielo.br/scielo.php?pid=S0100-40422009000900005&script=sci_arttext > Acesso em: 09 nov. 2013.
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