Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos do instituto biológico (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100241 |
Resumo: | ABSTRACT: Bacillus thuringiensis subsp. israelensis has been used to control the Aedes aegypti (Diptera: Culicidae) mosquito larvae, the vector of virus diseases such as dengue, Chikungunya and Zika fever, which have become a major public health problem in Brazil and other tropical countries since the climate favors the proliferation and development of the transmitting vector. Because B. thuringiensis has shown potential for controlling insects of the Diptera order, this work aimed at testing the Bacillus thuringiensis subsp. thuringiensis strain T01-328 and its proteins Cry2Aa and Cry2Ab for control A. aegypti and at comparing the results to the B. thuringiensis subsp. israelensis specific dipteran strain. To this end, bioassays using spore-crystal of both strains, and Cry2Aa and Cry2Ab proteins from the heterologous expression in Escherichia coli, were performed against A. aegypti larvae. The results showed that the B. thuringiensis thuringiensis T01-328 has insecticidal activity against the larvae, but it is less toxic than B. thuringiensis subsp. israelensis. Cry2Aa and Cry2Ab proteins expressed heterologously were effective for controlling A. aegypti larvae. Therefore, the results indicate that the Cry2Aa and Cry2Ab proteins of the B. thuringiensis thuringiensis T01-328 can be used as an alternative to assist in the control of A. aegypti. |
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Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae)Cry proteinsbiological controldengueZikamosquitoABSTRACT: Bacillus thuringiensis subsp. israelensis has been used to control the Aedes aegypti (Diptera: Culicidae) mosquito larvae, the vector of virus diseases such as dengue, Chikungunya and Zika fever, which have become a major public health problem in Brazil and other tropical countries since the climate favors the proliferation and development of the transmitting vector. Because B. thuringiensis has shown potential for controlling insects of the Diptera order, this work aimed at testing the Bacillus thuringiensis subsp. thuringiensis strain T01-328 and its proteins Cry2Aa and Cry2Ab for control A. aegypti and at comparing the results to the B. thuringiensis subsp. israelensis specific dipteran strain. To this end, bioassays using spore-crystal of both strains, and Cry2Aa and Cry2Ab proteins from the heterologous expression in Escherichia coli, were performed against A. aegypti larvae. The results showed that the B. thuringiensis thuringiensis T01-328 has insecticidal activity against the larvae, but it is less toxic than B. thuringiensis subsp. israelensis. Cry2Aa and Cry2Ab proteins expressed heterologously were effective for controlling A. aegypti larvae. Therefore, the results indicate that the Cry2Aa and Cry2Ab proteins of the B. thuringiensis thuringiensis T01-328 can be used as an alternative to assist in the control of A. aegypti.Instituto Biológico2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100241Arquivos do Instituto Biológico v.85 2018reponame:Arquivos do instituto biológico (Online)instname:Instituto Biológico (IB)instacron:IBIO10.1590/1808-1657000132018info:eu-repo/semantics/openAccessRicoldi,Marina CabralFigueiredo,Camila SoaresDesidério,Janete Apparecidaeng2018-11-21T00:00:00Zoai:scielo:S1808-16572018000100241Revistahttp://www.biologico.sp.gov.br/arquivos_bio.phphttps://old.scielo.br/oai/scielo-oai.php||arquivos@biologico.sp.gov.br1808-16570020-3653opendoar:2018-11-21T00:00Arquivos do instituto biológico (Online) - Instituto Biológico (IB)false |
dc.title.none.fl_str_mv |
Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae) |
title |
Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae) |
spellingShingle |
Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae) Ricoldi,Marina Cabral Cry proteins biological control dengue Zika mosquito |
title_short |
Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae) |
title_full |
Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae) |
title_fullStr |
Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae) |
title_full_unstemmed |
Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae) |
title_sort |
Toxicity of Cry2 proteins from Bacillus thuringiensis subsp. thuringiensis strain T01-328 against Aedes aegypti (Diptera: Culicidae) |
author |
Ricoldi,Marina Cabral |
author_facet |
Ricoldi,Marina Cabral Figueiredo,Camila Soares Desidério,Janete Apparecida |
author_role |
author |
author2 |
Figueiredo,Camila Soares Desidério,Janete Apparecida |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ricoldi,Marina Cabral Figueiredo,Camila Soares Desidério,Janete Apparecida |
dc.subject.por.fl_str_mv |
Cry proteins biological control dengue Zika mosquito |
topic |
Cry proteins biological control dengue Zika mosquito |
description |
ABSTRACT: Bacillus thuringiensis subsp. israelensis has been used to control the Aedes aegypti (Diptera: Culicidae) mosquito larvae, the vector of virus diseases such as dengue, Chikungunya and Zika fever, which have become a major public health problem in Brazil and other tropical countries since the climate favors the proliferation and development of the transmitting vector. Because B. thuringiensis has shown potential for controlling insects of the Diptera order, this work aimed at testing the Bacillus thuringiensis subsp. thuringiensis strain T01-328 and its proteins Cry2Aa and Cry2Ab for control A. aegypti and at comparing the results to the B. thuringiensis subsp. israelensis specific dipteran strain. To this end, bioassays using spore-crystal of both strains, and Cry2Aa and Cry2Ab proteins from the heterologous expression in Escherichia coli, were performed against A. aegypti larvae. The results showed that the B. thuringiensis thuringiensis T01-328 has insecticidal activity against the larvae, but it is less toxic than B. thuringiensis subsp. israelensis. Cry2Aa and Cry2Ab proteins expressed heterologously were effective for controlling A. aegypti larvae. Therefore, the results indicate that the Cry2Aa and Cry2Ab proteins of the B. thuringiensis thuringiensis T01-328 can be used as an alternative to assist in the control of A. aegypti. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100241 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100241 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1808-1657000132018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Biológico |
publisher.none.fl_str_mv |
Instituto Biológico |
dc.source.none.fl_str_mv |
Arquivos do Instituto Biológico v.85 2018 reponame:Arquivos do instituto biológico (Online) instname:Instituto Biológico (IB) instacron:IBIO |
instname_str |
Instituto Biológico (IB) |
instacron_str |
IBIO |
institution |
IBIO |
reponame_str |
Arquivos do instituto biológico (Online) |
collection |
Arquivos do instituto biológico (Online) |
repository.name.fl_str_mv |
Arquivos do instituto biológico (Online) - Instituto Biológico (IB) |
repository.mail.fl_str_mv |
||arquivos@biologico.sp.gov.br |
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1754193670328811520 |