Polymorphism C242T in the Cyp19 gene in meat sheep

Bibliographic Details
Main Author: Mora,N. H. A. P.
Publication Date: 2016
Other Authors: Silva,S. C. C., Tanamati,F., Schuroff,G. P., Macedo,F. A. F., Gasparino,E.
Format: Article
Language: eng
Source: Brazilian Journal of Biology
Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842016000100205
Summary: Abstract The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423).
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spelling Polymorphism C242T in the Cyp19 gene in meat sheepCyp19DorperpolymorphismSanta InêsTexelWhite DorperAbstract The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423).Instituto Internacional de Ecologia2016-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842016000100205Brazilian Journal of Biology v.76 n.1 2016reponame:Brazilian Journal of Biologyinstname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/1519-6984.17414info:eu-repo/semantics/openAccessMora,N. H. A. P.Silva,S. C. C.Tanamati,F.Schuroff,G. P.Macedo,F. A. F.Gasparino,E.eng2016-02-19T00:00:00Zoai:scielo:S1519-69842016000100205Revistahttps://www.scielo.br/j/bjb/https://old.scielo.br/oai/scielo-oai.phpbjb@bjb.com.br||bjb@bjb.com.br1678-43751519-6984opendoar:2016-02-19T00:00Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)false
dc.title.none.fl_str_mv Polymorphism C242T in the Cyp19 gene in meat sheep
title Polymorphism C242T in the Cyp19 gene in meat sheep
spellingShingle Polymorphism C242T in the Cyp19 gene in meat sheep
Mora,N. H. A. P.
Cyp19
Dorper
polymorphism
Santa Inês
Texel
White Dorper
title_short Polymorphism C242T in the Cyp19 gene in meat sheep
title_full Polymorphism C242T in the Cyp19 gene in meat sheep
title_fullStr Polymorphism C242T in the Cyp19 gene in meat sheep
title_full_unstemmed Polymorphism C242T in the Cyp19 gene in meat sheep
title_sort Polymorphism C242T in the Cyp19 gene in meat sheep
author Mora,N. H. A. P.
author_facet Mora,N. H. A. P.
Silva,S. C. C.
Tanamati,F.
Schuroff,G. P.
Macedo,F. A. F.
Gasparino,E.
author_role author
author2 Silva,S. C. C.
Tanamati,F.
Schuroff,G. P.
Macedo,F. A. F.
Gasparino,E.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Mora,N. H. A. P.
Silva,S. C. C.
Tanamati,F.
Schuroff,G. P.
Macedo,F. A. F.
Gasparino,E.
dc.subject.por.fl_str_mv Cyp19
Dorper
polymorphism
Santa Inês
Texel
White Dorper
topic Cyp19
Dorper
polymorphism
Santa Inês
Texel
White Dorper
description Abstract The objective of this study was to evaluate the frequency of C242T polymorphism on the aromatase gene and the allelic and genotypic frequency of these variants in sheep belonging to four breed groups. Blood samples were collected from 187 animals of four breed groups: Dorper, Santa Inês, Texel and White Dorper, originated from herds in the region of Maringá/PR, Brazil. The genomic DNA was extracted using alkaline extraction, with subsequent amplification of the fragments via PCR with specific primer. The samples resulting from amplification were subjected to digestion process using the Dpn II restriction enzyme and to polyacrylamide gel electrophoresis 10.0% and stained with silver nitrate. Three distinct genotypes were observed: homozygous (CC), heterozygous (CT) and homozygous for no cut (TT). The resulting data were analyzed using the POPGENE software with 5% significance. Genotypic frequencies among the breed groups were: Texel (CC - 0.426; CT - 0.511; TT - 0.064), Dorper (CC - 0.073; CT - 0.732; TT - 0.439), White Dorper (CC - 0.021; CT - 0.255; TT - 0.723) and Santa Inês (CC - 0.115; CT - 0.462; TT - 0.423).
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842016000100205
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842016000100205
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1519-6984.17414
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Internacional de Ecologia
publisher.none.fl_str_mv Instituto Internacional de Ecologia
dc.source.none.fl_str_mv Brazilian Journal of Biology v.76 n.1 2016
reponame:Brazilian Journal of Biology
instname:Instituto Internacional de Ecologia (IIE)
instacron:IIE
instname_str Instituto Internacional de Ecologia (IIE)
instacron_str IIE
institution IIE
reponame_str Brazilian Journal of Biology
collection Brazilian Journal of Biology
repository.name.fl_str_mv Brazilian Journal of Biology - Instituto Internacional de Ecologia (IIE)
repository.mail.fl_str_mv bjb@bjb.com.br||bjb@bjb.com.br
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