Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR

Detalhes bibliográficos
Autor(a) principal: Leonel, João Augusto Franco
Data de Publicação: 2021
Outros Autores: Tannihão, Bruna, Arantes, Julia Assis, Vioti, Geovanna, Benassi, Julia Cristina, Brandi, Roberta Ariboni, Ferreira, Helena Lage, Keid, Lara Borges, Soares, Rodrigo Martins, Oliveira, Trícia Maria Ferreira de Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/184826
Resumo: Visceral leishmaniasis (VL) is a neglected tropical disease caused by the Leishmania infantum parasite. The protozoan is able to infect several domestic and wild mammals. Since the first report on Leishmania spp. infection in horses in South America, leishmaniasis in equids has been highlighted in Brazil. A molecular epidemiological survey was carried out to verify the occurrence of Leishmania spp. DNA in horses and donkeys, in leishmaniases endemic areas in Sao Paulo State, Brazil. To this end, blood samples were obtained from 107 horses and 36 donkeys and subjected to DNA extraction followed by PCR targeting the ITS-1 region. Among the horses and donkeys, 1.87% (2/107) and 8.33% (3/36) were positive by PCR, respectively. The DNA sequencing of the ITS-1 amplification products confirmed L. infantum DNA in these animals. Our results suggest that horses and donkeys from non-VL and VL endemic areas of São Paulo State may be infected by the parasite.
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spelling Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCRAsininesEquinesEquidsLeishmania infantumITS-1PCRVisceral leishmaniasis (VL) is a neglected tropical disease caused by the Leishmania infantum parasite. The protozoan is able to infect several domestic and wild mammals. Since the first report on Leishmania spp. infection in horses in South America, leishmaniasis in equids has been highlighted in Brazil. A molecular epidemiological survey was carried out to verify the occurrence of Leishmania spp. DNA in horses and donkeys, in leishmaniases endemic areas in Sao Paulo State, Brazil. To this end, blood samples were obtained from 107 horses and 36 donkeys and subjected to DNA extraction followed by PCR targeting the ITS-1 region. Among the horses and donkeys, 1.87% (2/107) and 8.33% (3/36) were positive by PCR, respectively. The DNA sequencing of the ITS-1 amplification products confirmed L. infantum DNA in these animals. Our results suggest that horses and donkeys from non-VL and VL endemic areas of São Paulo State may be infected by the parasite.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2021-01-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/18482610.1590/s1678-9946202163012Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e12Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e12Revista do Instituto de Medicina Tropical de São Paulo; v. 63 (2021); e121678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/184826/170939Copyright (c) 2021 João Augusto Franco Leonel, Bruna Tannihão, Julia Assis Arantes, Geovanna Vioti, Julia Cristina Benassi, Roberta Ariboni Brandi, Helena Lage Ferreira, Lara Borges Keid, Rodrigo Martins Soares, Trícia Maria Ferreira de Sousa Oliveirahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLeonel, João Augusto Franco Tannihão, Bruna Arantes, Julia Assis Vioti, Geovanna Benassi, Julia Cristina Brandi, Roberta Ariboni Ferreira, Helena Lage Keid, Lara Borges Soares, Rodrigo Martins Oliveira, Trícia Maria Ferreira de Sousa 2022-05-16T13:44:35Zoai:revistas.usp.br:article/184826Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:56.735974Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR
title Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR
spellingShingle Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR
Leonel, João Augusto Franco
Asinines
Equines
Equids
Leishmania infantum
ITS-1
PCR
title_short Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR
title_full Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR
title_fullStr Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR
title_full_unstemmed Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR
title_sort Detection of Leishmania infantum DNA in blood samples of horses (Equus caballus) and donkeys (Equus asinus) by PCR
author Leonel, João Augusto Franco
author_facet Leonel, João Augusto Franco
Tannihão, Bruna
Arantes, Julia Assis
Vioti, Geovanna
Benassi, Julia Cristina
Brandi, Roberta Ariboni
Ferreira, Helena Lage
Keid, Lara Borges
Soares, Rodrigo Martins
Oliveira, Trícia Maria Ferreira de Sousa
author_role author
author2 Tannihão, Bruna
Arantes, Julia Assis
Vioti, Geovanna
Benassi, Julia Cristina
Brandi, Roberta Ariboni
Ferreira, Helena Lage
Keid, Lara Borges
Soares, Rodrigo Martins
Oliveira, Trícia Maria Ferreira de Sousa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leonel, João Augusto Franco
Tannihão, Bruna
Arantes, Julia Assis
Vioti, Geovanna
Benassi, Julia Cristina
Brandi, Roberta Ariboni
Ferreira, Helena Lage
Keid, Lara Borges
Soares, Rodrigo Martins
Oliveira, Trícia Maria Ferreira de Sousa
dc.subject.por.fl_str_mv Asinines
Equines
Equids
Leishmania infantum
ITS-1
PCR
topic Asinines
Equines
Equids
Leishmania infantum
ITS-1
PCR
description Visceral leishmaniasis (VL) is a neglected tropical disease caused by the Leishmania infantum parasite. The protozoan is able to infect several domestic and wild mammals. Since the first report on Leishmania spp. infection in horses in South America, leishmaniasis in equids has been highlighted in Brazil. A molecular epidemiological survey was carried out to verify the occurrence of Leishmania spp. DNA in horses and donkeys, in leishmaniases endemic areas in Sao Paulo State, Brazil. To this end, blood samples were obtained from 107 horses and 36 donkeys and subjected to DNA extraction followed by PCR targeting the ITS-1 region. Among the horses and donkeys, 1.87% (2/107) and 8.33% (3/36) were positive by PCR, respectively. The DNA sequencing of the ITS-1 amplification products confirmed L. infantum DNA in these animals. Our results suggest that horses and donkeys from non-VL and VL endemic areas of São Paulo State may be infected by the parasite.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/184826
10.1590/s1678-9946202163012
url https://www.revistas.usp.br/rimtsp/article/view/184826
identifier_str_mv 10.1590/s1678-9946202163012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/184826/170939
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e12
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e12
Revista do Instituto de Medicina Tropical de São Paulo; v. 63 (2021); e12
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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