The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus

Detalhes bibliográficos
Autor(a) principal: Motta, Maria Cristina Machado
Data de Publicação: 2010
Outros Autores: Preta, Carolina Moura Costa Catta, Schenkman, Sergio, Martins, Allan Cézar de Azevedo, Miranda, Kildare Rocha de, Souza, Wanderley de, Elias-Sabbaga, Maria Carolina Quartim Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional do INMETRO
Texto Completo: http://hdl.handle.net/10926/1929
Resumo: In trypanosomatids, cell division involves morphological changes and requires coordinated replication and segregation of the nucleus, kinetoplast and flagellum. In endosymbiont-containing trypanosomatids, like Crithidia deanei, this process is more complex, as each daughter cell contains only a single symbiotic bacterium, indicating that the prokaryote must replicate synchronically with the host protozoan. In this study, we used light and electron microscopy combined with threedimensional reconstruction approaches to observe the endosymbiont shape and division during C. deanei cell cycle. We found that the bacterium replicates before the basal body and kinetoplast segregations and that the nucleus is the last organelle to divide, before cytokinesis. In addition, the endosymbiont is usually found close to the host cell nucleus, presenting different shapes during the protozoan cell cycle. Considering that the endosymbiosis in trypanosomatids is a mutualistic relationship, which resembles organelle acquisition during evolution, these findings establish an excellent model for the understanding of mechanisms related with the establishment of organelles in eukaryotic cells.
id INMT_52196ae3faf992d9238b52cc99f3d7d5
oai_identifier_str oai:xrepo01s.inmetro.gov.br:10926/1929
network_acronym_str INMT
network_name_str Repositório Institucional do INMETRO
repository_id_str
spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus20102013-01-16T12:25:49Z2013-01-16T12:25:49ZIn trypanosomatids, cell division involves morphological changes and requires coordinated replication and segregation of the nucleus, kinetoplast and flagellum. In endosymbiont-containing trypanosomatids, like Crithidia deanei, this process is more complex, as each daughter cell contains only a single symbiotic bacterium, indicating that the prokaryote must replicate synchronically with the host protozoan. In this study, we used light and electron microscopy combined with threedimensional reconstruction approaches to observe the endosymbiont shape and division during C. deanei cell cycle. We found that the bacterium replicates before the basal body and kinetoplast segregations and that the nucleus is the last organelle to divide, before cytokinesis. In addition, the endosymbiont is usually found close to the host cell nucleus, presenting different shapes during the protozoan cell cycle. Considering that the endosymbiosis in trypanosomatids is a mutualistic relationship, which resembles organelle acquisition during evolution, these findings establish an excellent model for the understanding of mechanisms related with the establishment of organelles in eukaryotic cells.9 p. : il., tab.Submitted by Lívia Oliveira (livia.oliveira2@hotmail.com) on 2012-08-06T14:29:32Z No. of bitstreams: 1 Souza4_2010.pdf: 1630664 bytes, checksum: 708f440e4814e4439571b813682b2b96 (MD5)Approved for entry into archive by Regina Mello(mrmello@inmetro.gov.br) on 2013-01-16T12:25:49Z (GMT) No. of bitstreams: 1 Souza4_2010.pdf: 1630664 bytes, checksum: 708f440e4814e4439571b813682b2b96 (MD5)Made available in DSpace on 2013-01-16T12:25:49Z (GMT). No. of bitstreams: 1 Souza4_2010.pdf: 1630664 bytes, checksum: 708f440e4814e4439571b813682b2b96 (MD5) Previous issue date: 2010enghttp://hdl.handle.net/10926/1929DMD_hdl_10926/1929MOTTA, Maria Cristina Machado et al. The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus. Plos One, v. 5, n. 8, 2010.Motta, Maria Cristina MachadoPreta, Carolina Moura Costa CattaSchenkman, SergioMartins, Allan Cézar de AzevedoMiranda, Kildare Rocha deSouza, Wanderley deElias-Sabbaga, Maria Carolina Quartim Barbosainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETROMotta_2010.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1929/1/Motta_2010.pdfapplication/pdf1630664http://xrepo01s.inmetro.gov.br/bitstream/10926/1929/1/Motta_2010.pdf708f440e4814e4439571b813682b2b96MD510926_1929_1Motta_2010.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1929/6/Motta_2010.pdf.txttext/plain43215http://xrepo01s.inmetro.gov.br/bitstream/10926/1929/6/Motta_2010.pdf.txt426d482cc28871e4748983dba43f36deMD510926_1929_62024-06-10T15:24:56Zoai:xrepo01s.inmetro.gov.br:10926/1929Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2013-05-13T17:24:28Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false
dc.title.none.fl_str_mv The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus
title The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus
spellingShingle The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus
Motta, Maria Cristina Machado
title_short The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus
title_full The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus
title_fullStr The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus
title_full_unstemmed The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus
title_sort The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus
author Motta, Maria Cristina Machado
author_facet Motta, Maria Cristina Machado
Preta, Carolina Moura Costa Catta
Schenkman, Sergio
Martins, Allan Cézar de Azevedo
Miranda, Kildare Rocha de
Souza, Wanderley de
Elias-Sabbaga, Maria Carolina Quartim Barbosa
author_role author
author2 Preta, Carolina Moura Costa Catta
Schenkman, Sergio
Martins, Allan Cézar de Azevedo
Miranda, Kildare Rocha de
Souza, Wanderley de
Elias-Sabbaga, Maria Carolina Quartim Barbosa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Motta, Maria Cristina Machado
Preta, Carolina Moura Costa Catta
Schenkman, Sergio
Martins, Allan Cézar de Azevedo
Miranda, Kildare Rocha de
Souza, Wanderley de
Elias-Sabbaga, Maria Carolina Quartim Barbosa
description In trypanosomatids, cell division involves morphological changes and requires coordinated replication and segregation of the nucleus, kinetoplast and flagellum. In endosymbiont-containing trypanosomatids, like Crithidia deanei, this process is more complex, as each daughter cell contains only a single symbiotic bacterium, indicating that the prokaryote must replicate synchronically with the host protozoan. In this study, we used light and electron microscopy combined with threedimensional reconstruction approaches to observe the endosymbiont shape and division during C. deanei cell cycle. We found that the bacterium replicates before the basal body and kinetoplast segregations and that the nucleus is the last organelle to divide, before cytokinesis. In addition, the endosymbiont is usually found close to the host cell nucleus, presenting different shapes during the protozoan cell cycle. Considering that the endosymbiosis in trypanosomatids is a mutualistic relationship, which resembles organelle acquisition during evolution, these findings establish an excellent model for the understanding of mechanisms related with the establishment of organelles in eukaryotic cells.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.available.fl_str_mv 2013-01-16T12:25:49Z
dc.date.accessioned.fl_str_mv 2013-01-16T12:25:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10926/1929
DMD_hdl_10926/1929
dc.identifier.citation.fl_str_mv MOTTA, Maria Cristina Machado et al. The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus. Plos One, v. 5, n. 8, 2010.
url http://hdl.handle.net/10926/1929
identifier_str_mv DMD_hdl_10926/1929
MOTTA, Maria Cristina Machado et al. The bacterium endosymbiont of crithidia deanei undergoes coordinated division with the host cell nucleus. Plos One, v. 5, n. 8, 2010.
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.bitstream.fl_str_mv application/pdf
text/plain
dc.source.none.fl_str_mv reponame:Repositório Institucional do INMETRO
instname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)
instacron:INMETRO
instname_str Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)
instacron_str INMETRO
institution INMETRO
reponame_str Repositório Institucional do INMETRO
collection Repositório Institucional do INMETRO
repository.name.fl_str_mv Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)
repository.mail.fl_str_mv
_version_ 1801499966155259904