Inferência filogenética do segmento genômico S completo dos hantavirus identificados no Brasil

• Autor(a) principal: Silva, Alexandro Guterres da
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações do LNCC
• Texto Completo: https://tede.lncc.br/handle/tede/164
• topic: Estrutura molecular; Algoritmos genéticos; Molecular structure; Genetic algorithms; CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR

At the American continent several hantavirus cause hantavirus pulmonary syndrome, considered an emerging and important public health problem. In Brazil, since the first outbreak at the beginning of the 1990s, more than 1440 cases have been documented in 14 of the 27 states, with a mortality rate of 40 to 70%. Interestingly, despite the absence of reported cases at states of the Espírito Santo, Mato Grosso do Sul and Rio de Janeiro which bordering states with high incidence, different studies conducted had identified infected rodents reservoirs with pathogenic hantaviruses at this places, leading to a series of questions that couldn't be answered until this moment. The main objective of this study was to contribute to an understanding to the genetic diversity of hantaviruses identified in Brazil analyzing the complete sequence of genomic S segment. Initially a survey was conducted in online databases (GenBank) in order to obtain the complete nucleotide sequences of the S segment of different hantavirus, with emphasis on Brazilian sequences. From a total of 334 sequences retrieved, only 14 belonged to Brazil, all sequences recovered from human samples; 12 were genotype Araucaria (Juquitiba) and two, genotype Araraquara,. With this result, biological seroreactive animal samples, stored at Laboratório de Hantaviroses e Rickettsioses IOC FIOCRUZ, were selected and submitted to PCR. Primers were designed and developed to amplify the entire genomic S segment. The sequencing of 23 samples of wild rodents in endemic and non-endemic areas from seven Brazilian states made possible to provide (i) the first description and molecular characterization of a hantavirus in the state of the Espírito Santo, (ii) the first description of the genotype Juquitiba in the rodent species Oligoryzomys fornesi in Brazil, (iii) the complete sequence of the first S segment Jabora genotype. The phylogenetic analysis showed, in a unpublished way, the wide genetic diversity of Brazilian hantavirus. It wasn t possible to observe an association pattern between host or geography, confirming results obtained in available recent studies.